Comparative analysis of the microbiota of the cervical canal and fetoplacental complex at full-term pregnancy

Kaganova M.A.; Spiridonova N.V.; Michal’chenko S.V.

doi:https://doi.org/10.17116/rosakush20222205115

Kaganova M.A.

Samara State Medical University

Spiridonova N.V.

Samara State Medical University

Michal’chenko S.V.

Samara State Medical University

Comparative analysis of the microbiota of the cervical canal and fetoplacental complex at full-term pregnancy

Authors:

Kaganova M.A., Spiridonova N.V., Michal’chenko S.V.

More about the authors

Journal: Russian Bulletin of Obstetrician-Gynecologist. 2022;22(5): 15‑21

DOI: 10.17116/rosakush20222205115

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To cite this article:

Kaganova MA, Spiridonova NV, Michal’chenko SV. Comparative analysis of the microbiota of the cervical canal and fetoplacental complex at full-term pregnancy. Russian Bulletin of Obstetrician-Gynecologist. 2022;22(5):15‑21. (In Russ.)
https://doi.org/10.17116/rosakush20222205115

Подписка 2026 Российский вестник акушера-гинеколога (Russian Bulletin of Obstetrician-Gynecologist)

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OBJECTIVE

To analyze the microbial species of the cervical canal, amniotic fluid, fetal membranes and placenta during physiological full-term pregnancy.

MATERIAL AND METHODS

In 19 pregnant women with a physiological term pregnancy at 37—41 weeks’ gestation with intact membranes, the contents of the cervical canal were collected before elective caesarean section, then amniotic fluid, membrane and placental specimens were collected intraoperatively for DNA polymerase chain reaction detection of the following microorganisms: Lactobacillus spp, Staphylococcus spp., Gardnerella vaginalis/Prevotella bivia/Porphyromonas spp., Eubacterium spp., Sneathia spp./Leptotrihia spp./Fuso-bacterium spp., Megasphaera spp./Veillonella spp./Dialister spp, Lachnobacterium spp./Clostridium spp., Mobiluncus spp./Corynebacterium spp., Peptostreptococcus spp., Atopobi-um vaginae, Mycoplasma hominis, Ureaplasma (urealyticum + parvum), Candida spp., Myco-plasma genitalium.

RESULTS

In a physiological term pregnancy, DNA of Lactobacillus spp. was found in the cervical canal at 107.6 Ge per specimen [107.1; 108.2]. Total Laboratory Determined Bacterial Mass (TDBM) in amniotic fluid was detected in 10 of 19 pregnant women at 103.02 Ge per sample [0; 103.4], in fetal membranes at 14 observations at 103.2 Ge per sample [0; 103.3], and in the placenta at 15 observations at 103.3 Ge per sample [103.1; 103.5]. Microbial DNA unidentifiable by the Femoflor-16 panel was detected in the placenta in 15 observations, in fetal membranes in 9, and in amniotic fluid in 11 of the 19 examined. Among the microbes identified, the leading ones in placenta, membranes and amniotic fluid were Enterobacteraceae.

CONCLUSION

The feto-placental microflora has its own unique microbiome, distinct from that of the cervical canal.

Keywords:

pregnancy

femoflora

amniotic fluid

placenta

fetal membranes

cervical canal

polymerase chain reaction

DNA-technologies

microbiota

Authors:

Kaganova M.A.

Samara State Medical University

Spiridonova N.V.

Samara State Medical University

Michal’chenko S.V.

Samara State Medical University

Received:

25.04.2022

Accepted:

20.05.2022

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References:

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