The dynamics of erythrocytes functional indicators in patients with Charcot—Marie—Toota disease during cytoprotective therapy

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Objective. To study an impact of cytoflavin on the functional state of erythrocytes in patients with Charcot — Marie — Toota disease (CMTD). Material and methods. Thirty patients with CMTD were examined. The main group (n=17) received cytoflavin along with basic therapy (intravenously dropwise 10 ml per 200 ml of 5% glucose solution for 10 days). The comparison group (n=13) received only basic therapy. Functional parameters of erythrocytes, including electrophoretic mobility of erythrocytes (EFME), concentration of malonic dialdehyde (MDA), content of ATP and 2.3-DPG in erythrocytes, were evaluated. The level of physiological norm of the studied parameters was determined in 20 healthy people (the control group). To assess clinical symptoms, the MFI-20 scale and a 20 meter walking distance were used. Results and conclusion. There was a decrease in EFME and concentrations of ATP, 2.3 DFG and an increase in MDA concentration in the main group compared to the control group. After treatment with cytoflavin, there was an increase in the content of ATP, 2.3 DFG and EFME and a decrease in the concentration of MDA. The similar but less pronounced changes were observed in the comparison group. At the same time, the content of 2,3 DPG and the concentration of MDA did not reach the level of the physiological norm. The changes in functional indicators of erythrocytes were associated with clinical indicators. A significant decrease in the severity of asthenia according to the MFI-20 scale, an increase in walking speed, a decrease in the average walking time by 20 seconds (by 5 seconds in the comparison group) were observed in the main group. The results make it possible to classify the use of cytoflavin in CMTD as a method of pathogenetic therapy.

Keywords:

nonspecific cytoprotective therapy

cytoflavin

hereditary polyneuropathies

Charcot—Marie—Toota disease

functional parameters of erythrocytes

Authors:

Derugina A.V.

National Research University «Lobachevsky Nizhny Novgorod State University», Nizhny Novgorod

Antipenko E.A.

kafedra nevrologii, neĭrokhirurgii i psikhiatrii TsPK i PPS Nizhegorodskoĭ gosudarstvennoĭ meditsinskoĭ akademii

SPIN RSCI: 7708-9068
Scopus AuthorID: 34871353400
ORCID: 0000-0002-8972-9150

List of references:

Levin OS. Polyneuropathies: a political guide. M.: Medicinskoe informacionnoe agentstvo; 2011. (In Russ.)
Fel’dman E. Atlas of Neuromuscular Diseases: A Practical Guide. M.: Prakticheskaya medicina; 2016. (In Russ.)
Kovalenko AL. The use of cytoflavin in toxic and posthypoxic encephalopathy. Manual for doctors and students. SPb.: Taktik-Studio; 2004. (In Russ.)
Kashuro VA, Glushkov SI, ZHeregelya SN. Correction of side effects of polychemotherapy and antioxidant status by the drug «Cytoflavin» in patients with breast cancer. Vestnik Sankt-Peterburgskoj Gosudarstvennoj Medicinskoj Akademii im. I.I. Mechnikova. 2009;3:124-128. (In Russ.)
Mazin PV, SHeshunov IV, Mazina NK. Meta-analysis of clinical efficacy of cytoflavin in neurological diseases. Zhurnal Nevrologii i Psihiatrii im. S.S Korsakova. 2017;117(3):28-39 (In Russ.) https://doi.org/10.17116/jnevro201711731
Pustozerov VG, Sajkova LA, Ledashcheva TA, ZHulev NM. Modern aspects of diagnostics, treatment and rehabilitation of hereditary neuromuscular diseases (experience of the Department and Clinic of Nervous Diseases SPBMAPO). Vestnik Sankt-Peterburgskoj Medicinskoj Akademii Poslediplomnogo Obrazovaniya. 2009;1(1) 96-100. (In Russ.)
Boyarinov GA, Deryugina AV, Yakovleva EI, Zaitsev RR, Shumilova AV, Bugrova ML, Boyarinova LV, Filippenko ES, Soloveva OD. Pharmacological correction of microcirculation in rats suffered from traumatic brain injury. Cell Tiss Biol. (Tsitologiia). 2017;11(1):65-72. (In Russ.)
Baskurt OK, Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ. Handbook of hemorheology and hemodynamics. IOS Press. 2007.
Krylov VN, Deryugina AV, Pleskova SN. Electrophoretic mobility of erythrocytes and morphometry of rat erythrocytes under stressful influences. Sovremennye Tekhnologii v Medicine. 2010;4:23-26. (In Russ.)
Antipenko EA, Deryugina AV, Gustov AV. The influence of nonspecific cytoprotective therapy on the stress-resistance and compensatory capabilities of patients with chronic cerebral ischemia. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2015;115(12):74-78. (In Russ.) https://doi.org/10.17116/jnevro201511511274-78
Deryugina AV, Boyarinov GA, Simutis IS, Nikol’skij VO, Kuznecov AB, Efimova TS. Correction of the erythrocyte mass of erythrocyte metabolic indicators and myocardial structure after acute blood loss. Citologiya. 2018;60(2):89-95. (In Russ.)
Krylov VN, Deryugina AV, Pleskova SN, Kalinin VA. The apoptotic nature of hemolysis of erythrocytes, inducironnyh small doses of ionizing radiation. Biofizika. 2015;60(1):102-108. (In Russ.)
Sprague R, Ellsworth M, Stephenson A, Kleinhenz M. Deformation-induced ATP release from red blood cells requires CFTR activity. American Physio-logical Society. 2010;26(2):168-174.
Glushkova EG, Glushkov VS, Kalinin EP, Galyan SL. Change in permeability of erythrocyte membranes for ATP under their shear deformation under conditions of activation of free radical oxidation. Medicinskaya Nauka i Obrazovanie Urala. 2016;17(3):40-43. (In Russ.)
Derpak YuYu. The state of energy metabolism in red blood cells of blood donors. Medicinskaya Nauka i Obrazovanie Urala. 2014;4:43-46. (In Russ.)
Mattecci E, Cocci F, Pellegrini L, Gregori G, Navalesi R, Giampietro O. Erythrocyte ATPase enzymes family in normal people. Eur J Clin Jnvest. 1992;22(4):11-18.
Takakuwa Y. Protein 4.1, a multifunctional protein of the erythrocyte membrane skeleton: structure and functions in erythrocytes and nonerythroid cells. Int J Hematol. 2000;72(3):298-309.
Nunomura W, Takakuwa Y. Regulation of protein 4.1R interactions with membrane proteins by Ca2+ and calmodulin. Frontiers in Bioscience. 2006;11:1522-1539.
An X, Mohandas N. Disordes of red cell membrane. Br J Haematol. 2008;14(3):367-375.
Ovchinnikova OA, Tihomirova IA. Rheological properties of blood in conditions of modification of the energy balance of erythrocytes. Yaroslavskij Pedagogicheskij Vestnik. 2012;3:134-139. (In Russ.)
Oliveira S, Silva-Herdade AS and Saldanha C. Modulation of erythrocyte deformability by PKC activity. Clin Hemorheol and Microcirculation. 2008;39:363-373.
Murav’ev AV, Komlev VL, Mihajlov PV, Ahapkina AA, Murav’ev AA. Deformation of erythrocytes: a role in microcirculation. Yaroslavskij Pedagogicheskij Vestnik. 2013;3(2):93-102. (In Russ.)
Bryzgalov NYu, Brazhe NA, Yusipovich AI, Maksimov GV, Rubin AB. The role of cytoplasmic structures of erythrocytes in the change in the affinity of hemoglobin for oxygen. Biofizika. 2009;54(3):442-447. (In Russ.)

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