The role of gene-gene and gene-environmental interactions of polymorphic matrix metalloproteinases loci in the formation of the risk of stroke in men with hypertension

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OBJECTIVE

Analysis of the role of genetic polymorphisms of matrix metalloproteinases (MMPs), their gene-gene and gene-environment interactions in the formation of ischemic stroke in men with arterial hypertension (AI).

MATERIAL AND METHODS

The study included 523 men with arterial hypertension: 201 patients with ischemic stroke and 322 patients without stroke. The association of MMPs loci with stroke with hypertension was determined by logistic regression analysis in dominant, recessive, additive genetic models in PLINK v.2.050. For five SNPs co-located on chromosome 11 (314.3 kb), haplotypic analysis was performed, the relationship of haplotypes with stroke development was determined by the EM algorithm. Gene-gene and gene-environment interactions of MMPs with smoking and alcohol consumption during stroke development were evaluated by GMDR (Generalized Multifactor Dimensionality Reduction) using GMDR v.0.9 software.

RESULTS

Polymorphic locus rs3025058 is associated with stroke in men in dominant and additive genetic models (OR=0.63—0.74, p_perm=0.03). Four haplotypes of MMPs have a protective effect on the development of stroke with hypertension (OR=0.48—0.50, p_perm=0.02—0.03). Four models of gene-gene interactions of polymorphic MMPs loci (OR=2.19—2.55, p_perm<0.001) and three four-factor models of gene-environment interactions of MMPs with alcohol abuse (OR=2.82—3.11, p_perm<0.001) are associated with a high risk of ischemic stroke in men with hypertension. rs3025058, rs1320632, rs11225395 and rs1799750 demonstrate the greatest contribution to gene-gene and gene-environment interactions in the formation of AI.

CONCLUSION

Thus, the results of the study indicate that the interactions of MMPs genes with each other and with modifiable environmental factors play a significant role in the development of stroke with hypertension in men.

Keywords:

ischemic stroke

matrix metalloproteinases

gene-environment interactions

Authors:

Kalinina M.I.

Belgorod National Research University

Ponomarenko I.V.

Belgorod State National Research University

ORCID: 0000-0002-5652-0166

Efremova O.A.

Belgorod State National Research University

ORCID: 0000-0003-4967-2556

Batlutskaya I.V.

Belgorod State National Research University

ORCID: 0000-0003-0068-6586

Churnosov M.I.

Belgorod State National Research University

ORCID: 0000-0003-1254-6134

Received:

02.02.2022

Accepted:

02.02.2022

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