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Kastyro I.V.

People’s Friendship University of Russia

Khamidulin G.V.

FSAEI HE Peoples’ Friendship University of Russia

Dyachenko Yu.E.

Peoples’ Friendship University of Russia

Kostyaeva M.G.

Peoples’ Friendship University of Russia (RUDN University)

Tsymbal A.A.

FSAEI HPE “I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation” (Sechenov University)

Shilin S.S.

Peoples’ Friendship University of Russia (RUDN University)

Popadyuk V.I.

Peoples’ Friendship University of Russian

Mikhalskaya P.V.

FSAEI HE Peoples’ Friendship University of Russia

Ganshin I.B.

FSAEI HE Peoples’ Friendship University of Russia

Analysis of p53 protein expression and formation of dark neurons in the hippocampus of rats during septoplasty modeling

Authors:

Kastyro I.V., Khamidulin G.V., Dyachenko Yu.E., Kostyaeva M.G., Tsymbal A.A., Shilin S.S., Popadyuk V.I., Mikhalskaya P.V., Ganshin I.B.

More about the authors

Journal: Russian Rhinology. 2023;31(1): 27‑36

DOI: 10.17116/rosrino20233101127

Read: 1748 times

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Table of contents

ANALYSIS OF P53 PROTEIN EXPRESSION AND FORMATION OF DARK NEURONS IN THE HIPPOCAMPUS OF RATS DURING SEPTOPLASTY MODELING
ANALYSIS OF P53 PROTEIN EXPRESSION AND FORMATION OF DARK NEURONS IN THE HIPPOCAMPUS OF RATS DURING SEPTOPLASTY MODELING

To cite this article:

Kastyro IV, Khamidulin GV, Dyachenko YuE, et al. . Analysis of p53 protein expression and formation of dark neurons in the hippocampus of rats during septoplasty modeling. Russian Rhinology. 2023;31(1):27‑36. (In Russ.)
https://doi.org/10.17116/rosrino20233101127

Подписка 2026 Российская ринология (Russian Rhinology)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

AIM OF THE STUDY

Determining the role of surgical stress in the formation of p53-positive and dark neurons (DN) in the hippocampus; analyzing their parallel formation in the pyramid hippocampus layer.

MATERIAL AND METHODS

Septoplasty was modeled in 20 mature Wistar male rats. Histological hippocampus slices were analyzed, where the number of DN and p53-positive neurons was determined on Days 2, 4, and 6 after the surgery.

RESULTS

Compared to the control group (5 animals), the number of DN and p53-positive neurons in experimental animals increased in all periods. A direct relation between the increased number of DN and p53-positive neurons was confirmed.

CONCLUSION

Septoplasty modeling in rats leads to the activation of pathological cascades, which in turn changes the multifunctional properties of neurons in the pyramidal hippocampus layer and promotes their neuroplasticity. The activation of neuronal NMDS-receptors during stress possibly initiates two pathways of neuronal life — the onset of p53 protein expression and the formation of DN. Both pathways may finally lead to apoptosis. The formation of DN and the p53 protein expression in them are most likely related and may possibly provide the neuroprotective mechanisms.

Keywords:

septoplasty
hippocampus
dark neurons
p53
stress

Authors:

Kastyro I.V.

People’s Friendship University of Russia

Khamidulin G.V.

FSAEI HE Peoples’ Friendship University of Russia

Dyachenko Yu.E.

Peoples’ Friendship University of Russia

Kostyaeva M.G.

Peoples’ Friendship University of Russia (RUDN University)

Tsymbal A.A.

FSAEI HPE “I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation” (Sechenov University)

Shilin S.S.

Peoples’ Friendship University of Russia (RUDN University)

Popadyuk V.I.

Peoples’ Friendship University of Russian

Mikhalskaya P.V.

FSAEI HE Peoples’ Friendship University of Russia

Ganshin I.B.

FSAEI HE Peoples’ Friendship University of Russia

Received:

15.07.2022

Accepted:

03.11.2022

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