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Fedosova D.V.

Pavlov First Saint Petersburg State Medical University

Aleksandrova M.S.

N.N. Petrov National Medicine Research Center of Oncology

Garanin A.Yu.

N.N. Petrov National Medicine Research Center of Oncology

Zabegina L.M.

N.N. Petrov National Medicine Research Center of Oncology

Kruglov S.Y.

Pavlov First Saint Petersburg State Medical University

Katsuba K.E.

N.N. Petrov National Medicine Research Center of Oncology

Shalaev A.V.

N.N. Petrov National Medicine Research Center of Oncology

Lokteva P.A.

N.N. Petrov National Medicine Research Center of Oncology

Polkovnikova I.A.

City Hospital No. 40

Apalko S.V.

City Hospital No. 40

Scherbak S.G.

City Hospital No. 40

Bezhenar V.F.

Pavlov First Saint Petersburg State Medical University

Malek A.V.

N.N. Petrov National Medicine Research Center of Oncology;
Algimed-Techno LLC

Diagnostic potential of small non-coding RNAs in pipelle biopsy material of eutopic endometrium

Authors:

Fedosova D.V., Aleksandrova M.S., Garanin A.Yu., Zabegina L.M., Kruglov S.Y., Katsuba K.E., Shalaev A.V., Lokteva P.A., Polkovnikova I.A., Apalko S.V., Scherbak S.G., Bezhenar V.F., Malek A.V.

More about the authors

Journal: Russian Journal of Human Reproduction. 2026;31(1): 92‑102

DOI: 10.17116/repro20263201192

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Table of contents

DIAGNOSTIC POTENTIAL OF SMALL NON-CODING RNAS IN PIPELLE BIOPSY MATERIAL OF EUTOPIC ENDOMETRIUM
DIAGNOSTIC POTENTIAL OF SMALL NON-CODING RNAS IN PIPELLE BIOPSY MATERIAL OF EUTOPIC ENDOMETRIUM

To cite this article:

Fedosova DV, Aleksandrova MS, Garanin AYu, et al. Diagnostic potential of small non-coding RNAs in pipelle biopsy material of eutopic endometrium. Russian Journal of Human Reproduction. 2026;31(1):92‑102. (In Russ.)
https://doi.org/10.17116/repro20263201192

 
Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

BACKGRAUND

Despite the high prevalence of endometriosis and the social significance of the endometriosis-associated infertility issue, reliable molecular markers of this disease are not known. Members of heterogeneous group of small non-coding RNA appear to be promising markers of the disease, and analysis of these molecules in eutopic endometrium can be a basis of innovative diagnostic technology.

OBJECTIVE

To conduct a comparative analysis of known classes of small non-coding RNAs expression in the material of the eutopic endometrium of patients and healthy women.

MATERIAL AND METHODS

The study was carried out using «deep» sequencing, the results obtained were verified using microarray PCR — a technology optimal for creating new diagnostic solutions at the next stages of work. Objects of the study — eutopic endometrium samples of patients with external genital endometriosis (n=45) and healthy women (n=24).

RESULTS

Potential marker microRNA (microRNA), piRNA (piwi-RNA), small nucleolar RNA (snoRNA) and small nuclear RNA (snRNA) were identified. The difference in the expression activity of a number of microRNAs (miR-143-3p, -320a-3p, -4488, -let-7b-5p, -451a, -16-5p) in the eutopic endometrium of patients with endometriosis vs. healthy women was verified. The high diagnostic potential of the concentration ratio of a number of so-called «miRNA reciprocal pairs» (for example, miR-12136/-320a, AUC = 0.89) was demonstrated.

CONCLUSIONS

Analysis of short regulatory RNA in eutopic endometrial is a promising PCR-based technology for minimally invasive diagnosis of endometriosis.

Keywords:

endometriosis
microRNA
diagnosis
PCR
eutopic endometrium

Authors:

Fedosova D.V.

Pavlov First Saint Petersburg State Medical University

Aleksandrova M.S.

N.N. Petrov National Medicine Research Center of Oncology

Garanin A.Yu.

N.N. Petrov National Medicine Research Center of Oncology

Zabegina L.M.

N.N. Petrov National Medicine Research Center of Oncology

Kruglov S.Y.

Pavlov First Saint Petersburg State Medical University

Katsuba K.E.

N.N. Petrov National Medicine Research Center of Oncology

Shalaev A.V.

N.N. Petrov National Medicine Research Center of Oncology

Lokteva P.A.

N.N. Petrov National Medicine Research Center of Oncology

Polkovnikova I.A.

City Hospital No. 40

Apalko S.V.

City Hospital No. 40

Scherbak S.G.

City Hospital No. 40

Bezhenar V.F.

Pavlov First Saint Petersburg State Medical University

Malek A.V.

N.N. Petrov National Medicine Research Center of Oncology;
Algimed-Techno LLC

Received:

03.12.2024

Accepted:

18.03.2025

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