Method of photodynamic action in surgical treatment of local limited peritonitis in the experiment

Musayelyan A.G.; Alipov V.V.; Tuchina E.S.; Rykhlov A.S.; Alipov A.I.; Gritsai U.O.; Gadzhieva E.E.; Gasparyan B.T.; Mylnikov A.M.

doi:https://doi.org/10.17116/operhirurg2025902277

Musayelyan A.G.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0002-9849-7304

Alipov V.V.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0002-1859-0825

Tuchina E.S.

Saratov National Research State University named after N.G. Chernyshevsky

ORCID: 0000-0003-4498-2846

Rykhlov A.S.

Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov

ORCID: 0000-0003-1194-9548

Alipov A.I.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0003-4526-0503

Gritsai U.O.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0006-9932-7602

Gadzhieva E.E.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0002-0154-0415

Gasparyan B.T.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0008-1740-2053

Mylnikov A.M.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0000-0003-2433-8228

Method of photodynamic action in surgical treatment of local limited peritonitis in the experiment

Authors:

Musayelyan A.G., Alipov V.V., Tuchina E.S., Rykhlov A.S., Alipov A.I., Gritsai U.O., Gadzhieva E.E., Gasparyan B.T., Mylnikov A.M.

More about the authors

Journal: Russian Journal of Operative Surgery and Clinical Anatomy. 2025;9(2‑2): 77‑86

DOI: 10.17116/operhirurg2025902277

Read: 930 times

Contact the author

Table of contents

To cite this article:

Musayelyan AG, Alipov VV, Tuchina ES, et al. Method of photodynamic action in surgical treatment of local limited peritonitis in the experiment. Russian Journal of Operative Surgery and Clinical Anatomy. 2025;9(2‑2):77‑86. (In Russ.)
https://doi.org/10.17116/operhirurg2025902277

Подписка 2026 Оперативная хирургия и клиническая анатомия (Пироговский научный журнал) (Russian Journal of Operative Surgery and Clinical Anatomy)

Использование инфографики авторами научных работ

BACKGROUND

Despite the use of antibiotics and metal nanoparticles, mortality in surgical treatment of local limited peritonitis (LLP) remains high, therefore, it is advisable to develop a new method of using photodynamic therapy (PDT) in the postoperative period.

OBJECTIVE

To substantiate a new method of using PDT in postoperative treatment of simulated MOP.

MATERIAL AND METHODS

The material for this study was 80 white laboratory rats weighing 200±50 g, which were divided into four groups (20 animals in each). After modeling MOP, the animals of all experimental groups underwent opening, sanitization and drainage of the purulent cavity. In the postoperative two-week period of MOP treatment, laboratory, planimetric, microbiological and instrumental research methods were used. In the control group, standard sanitization of the cavity and antibiotic treatment were performed. In the second group, the cavity was treated with a mixture of intralipid solutions and low-intensity laser radiation (LILI). In the third group, the MOP cavity was treated with metal nanoparticles in an intralipid solution followed by LILI. PDT in the treatment of localized peritonitis in animals of the fourth group included a mixture of intralipid and methylene blue solutions (as a photosensitizer) and irradiation with red LED radiation.

RESULTS

When comparing the results obtained by the 14th day of treatment of localized peritonitis, only in animals of the fourth group normalization of the OAC parameters, complete suppression of microflora growth, obliteration of the cavity due to proliferation, replacement with collagen fibers and connective tissue were achieved.

CONCLUSION

A treatment method that allows complete obliteration of the localized peritonitis cavity and shortening the treatment period has been experimentally substantiated.

Keywords:

localized peritonitis

diagnostics

treatment

photodynamic therapy

Authors:

Musayelyan A.G.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0002-9849-7304

Alipov V.V.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0002-1859-0825

Tuchina E.S.

Saratov National Research State University named after N.G. Chernyshevsky

ORCID: 0000-0003-4498-2846

Rykhlov A.S.

Saratov State University of Genetics, Biotechnology and Engineering named after N.I. Vavilov

ORCID: 0000-0003-1194-9548

Alipov A.I.

V.I. Razumovsky Saratov State Medical University State Medical University

ORCID: 0000-0003-4526-0503

Gritsai U.O.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0006-9932-7602

Gadzhieva E.E.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0002-0154-0415

Gasparyan B.T.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0009-0008-1740-2053

Mylnikov A.M.

Saratov State Medical University named after V.I. Razumovsky

ORCID: 0000-0003-2433-8228

Received:

30.09.2024

Accepted:

14.11.2024

Close metadata

References:

Alipov VV, Avanesyan GA, Musaelyan AG, Alipov AI, Mustafaeva DR. Modern problems of modelling and treatment of soft tissues abscesses. Pirogov Russian Journal of Surgery. 2020;(5):81-86. (In Russ.). https://doi.org/10.17116/hirurgia202005181
Alipov VV, Lebedev MS, Avanesyan GA, Musayelyan AG, Mustafayeva DR, Alipov AI. Experimental model of purulent liver abscess. Operative surgery and clinical anatomy (Pirogov Scientific Journal). 2019; 3(1):3-7. (In Russ.). https://doi.org/10.17116/operhirurg201930113
Alipov VV, Shapoval OG, Polidanov MA, Kondrashkin IE, Blokhin IS, Rasulov ISh, Karsakov VA, Tirbulatov TA, Musaelyan AG, Alipov AI. Antibacterial Activity of Copper, Iron, Zinc Nanoparticles and Low-Intensity Laser Radiation Used Separately and Together. Ovchinnikov Bulletin of Biotechnology and Physicochemical Biology. 2023; 19(1):58-64.
Mustafaev RD, Ibragimov TR, Derbenev VA, Tikhov GV. Antibacterial properties of pdt in experimental peritonitis. Federal State Budgetary Institution “O.K. Skobelkin State Research Center of Laser Medicine of the Federal Medical and Biological Agency of Russia”, Moscow, Research Institute of Surgery named after Academician M.A. Topchibashev, Baku. Eurasian Union of Scientists (ESU). 2019;5(62).
Yang Z, Hu X, Zhou L, He Y, Zhang X, Yang J, Ju Z, Liou YC, Shen HM, Luo G, Hamblin MR, He W, Yin R. Photodynamic therapy accelerates skin wound healing through promoting re-epithelialization. Burns Trauma. 2021; 9:tkab008. https://doi.org/10.1093/burnst/tkab008
Heckenkamp J, Adili F, Kishimoto J, Koch M, Lamuraglia GM. Local photodynamic action of methylene blue favorably modulates the postinterventional vascular wound healing response. J Vasc Surg. 2000;31(6):1168-1177. https://doi.org/10.1067/mva.2000.106491
Zuhairi H, Nikolaev, Knyazkova VV, Lepekhina AI, Krivova TB, Tuchin NA, VV, Kistenev YuV. Quantitative assessment of the in vivo efficacy of local low-dose photodynamic therapy in wound healing using two-photon microscopy. Pharmaceuticals. 2022;14:287. https://doi.org/10.3390/pharmaceutics14020287
Sun Y, Tosa M, Takada H, Ogawa R. Photodynamic Therapy Delays Cutaneous Wound Healing in Mice. J Nippon Med Sch. 2020;87(3):110-117. https://doi.org/10.1272/jnms.JNMS.2020_87-301
Tolstykh PI, Derbenev VA, Kuleshov IIu, Azimshoev AM, Tamrazova OB, Romanova AS, Karakhanov TI, Solov’eva AB. Laser photodynamic therapy of septic wounds. Pirogov Russian Journal of Surgery. 2010;12:17-22. (In Russ.).
Petrishchev NN, Galkin MA, Grishacheva TG, Dementyeva IN, Chefu S.G. Effect of a drug based on chlorin e6 on platelet aggregation activity. Biomedical Photonics. 2019;8(3):4-10. https://doi.org/10.24931/2413-9432-2019-8-3-4-10
Gunaydin G, Gedik Me and Ayan S. Photodynamic therapy for cancer treatment and diagnosis. A review of the current clinical status. Front Chem. 2021;9:686303. https://doi.org/10.3389/fchem.2021.686303
Rynda A.Yu., Olyushin VE, Rostovtsev DM, Zabrodskaya YuM, Tastanbekov MM, Papayan GV. Results of using intraoperative fluorescence control with chlorin E6 in resection of glial brain tumors. Burdenko’s Journal of Neurosurgery. 2021;85(4):20-28. https://doi.org/10.17116/neiro20218504120
Brokinkel B, Holling M, Grauer O, Suero Molina E, Warneke N, Stummer W. Combination of ALAinduced fluorescence-guided resection and intraoperative open photodynamic therapy for recurrent glioblastoma: case series on a promising dual strategy for local tumor control. J Neurosurg. 2021;134(2):426-436.
Dupont C, Vermandel M, Leroy HA, Quidet M, Lecomte F, Delhem N, Mordon S, Reyns N. Intraoperative photodynamic Therapy for Glioblastomas (INDYGO): Study Protocol for a Phase I Clinical Trial. Neurosurgery. 2019;84(6):414-419. https://doi.org/10.1093/neuros/nyy324
Rynda AYu, Olyushin VE, Rostovtsev DM, Zabrodskaya YuM, Ulitin AYu, Papayan GV. Intraoperative photodynamic therapy in complex treatment of malignant gliomas. Burdenko’s Journal of Neurosurgery. 2023;87(1):25-34. (In Russ.). https://doi.org/10.17116/neiro20238701125
Alipov VV, SV Kapralov, MS Lebedev [et al.]. Method of transcutaneous puncture of focal lesions of parenchymatous organs. Patent No. 2393812 C1 Russian Federation, IPC A61B 18/22.: No. 2008149361/14: declared 15.12.2008: published 10.07.2010; applicant State Educational Institution of Higher Professional Education “Saratov State Medical University” of the Federal Agency for Healthcare and Social Development. EDN QPJHGI. (In Russ.). https://yandex.ru/patents/doc/RU2393812C1_20100710
Alipov VV, Rykhlov AS, Musaelyan AG, Takhmezov AE, Polidanov MA, Kondrashkin IE, Volkov KA, Karsakov VA, Alipov AI. Method of modeling and treating abdominal abscess in a surgical experiment. Russian Journal of Operative Surgery and Clinical Anatomy. 2023; 7(2):5-12. https://doi.org/10.17116/operhirurg202370215
Patent No. 2822708 C1 Russian Federation, IPC A61B 17/00, A61N 5/067, G09B 23/28. Method for combined treatment of local limited peritonitis in an experiment: No. 2023127755: declared 10/27/2023: published 07/11/2024. VV Alipov, AG Musaelyan, MA Polidanov [et al.]; applicant Federal State Budgetary Educational Institution of Higher Education “V.I. Razumovsky Saratov State Medical University” of the Ministry of Health of the Russian Federation. — EDN ZYSUHL.