Endometrial expression of leukemia-inhibiting factor in women of reproductive age with different endometrial thickness

Aganezova N.V.; Aganezov S.S.; Gogichashvili K.E.; Artemyeva A.S.; Niuganen A.O.

doi:https://doi.org/10.17116/repro2023290416

Aganezova N.V.

Mechnikov North-Western State Medical University

SPIN RSCI: 2961-5377
Scopus AuthorID: 57191916251
ORCID: 0000-0002-9676-1570

Aganezov S.S.

Mechnikov North-Western State Medical University

SPIN RSCI: 8186-6778
Scopus AuthorID: 55744676600
ORCID: 0000-0002-3523-9922

Gogichashvili K.E.

North-Western State Medical University named after I.I. Mechnikov

ORCID: 0000-0002-5430-7118

Artemyeva A.S.

N.N. Petrov National Medical Research Center for Oncology

ORCID: 0000-0002-2948-397X

Niuganen A.O.

National Medical Research Center of Oncology named after N.N. Petrov

ORCID: 0000-0003-2685-5093

Endometrial expression of leukemia-inhibiting factor in women of reproductive age with different endometrial thickness

Authors:

Aganezova N.V., Aganezov S.S., Gogichashvili K.E., Artemyeva A.S., Niuganen A.O.

More about the authors

Journal: Russian Journal of Human Reproduction. 2023;29(4): 6‑17

DOI: 10.17116/repro2023290416

Read: 1801 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Aganezova NV, Aganezov SS, Gogichashvili KE, Artemyeva AS, Niuganen AO. Endometrial expression of leukemia-inhibiting factor in women of reproductive age with different endometrial thickness. Russian Journal of Human Reproduction. 2023;29(4):6‑17. (In Russ.)
https://doi.org/10.17116/repro2023290416

Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)

Использование инфографики авторами научных работ

Close metadata

THE AIM OF THE STUDY

Was to evaluate the expression of leukemia-inhibiting factor (LIF) in the endometrium during the «implantation window» in women with a history of reproductive dysfunction with different endometrial thickness.

MATERIAL AND METHODS

The prospective cohort comparative study was conducted. The main group included patients with hypoplastic (<7 mm according to ultrasound on preovulatory days) endometrium (n=52); the comparison group included women with normal endometrial thickness (n=62) (women of both groups with reproductive dysfunctions of unknown reason); the control group included 16 healthy fertile women. Aspiration biopsy of the endometrium was performed on the 6th-8th day after ovulation with simultaneous collection of a peripheral blood sample to determine the levels of estradiol (E₂) and progesterone (P). Histological and immunohistochemical examination of endometrial samples was performed to determine the expression of estrogen (ER) and progesterone (PR) receptors and LIF.

RESULTS

All study participants had ovulatory progesterone values (P≥16.1 nmol/l (6—8 days after ovulation)) and normoestrogenemia (E₂, pmol/l). E₂/P was similar in all studied groups (p>0.05). We found, that 20% of women with «thin» endometrium have expression of LIF similar to healthy women. We report these results for women with «thin» endometrium who have normal hormone-receptor characteristics and medium secretory phase transformation of the endometrium.

CONCLUSION

In 20% of women with a history of reproductive dysfunctions with a «thin» endometrium, the expression of sex steroid receptors, proteomic markers (LIF) and phase transformation of the endometrium were the same as in healthy women. «Thin» endometrium is a predictor of a greater percentage of reproductive failures but does not appear to be an absolute predictor of such disorders. There is a group of women with hypoplastic endometrial syndrome who have adequate characteristics of endometrial receptivity and who able to become pregnant in a natural menstrual cycle.

Keywords:

endometrial receptivity

estrogen receptors

progesterone receptors

hypoplastic endometrium

leukemia-inhibiting factor

infertility

miscarriage

Authors:

Aganezova N.V.

Mechnikov North-Western State Medical University

SPIN RSCI: 2961-5377
Scopus AuthorID: 57191916251
ORCID: 0000-0002-9676-1570

Aganezov S.S.

Mechnikov North-Western State Medical University

SPIN RSCI: 8186-6778
Scopus AuthorID: 55744676600
ORCID: 0000-0002-3523-9922

Gogichashvili K.E.

North-Western State Medical University named after I.I. Mechnikov

ORCID: 0000-0002-5430-7118

Artemyeva A.S.

N.N. Petrov National Medical Research Center for Oncology

ORCID: 0000-0002-2948-397X

Niuganen A.O.

National Medical Research Center of Oncology named after N.N. Petrov

ORCID: 0000-0003-2685-5093

Received:

13.02.2023

Accepted:

10.05.2023

Close metadata

References:

Zhenskoe besplodie (sovremennye podkhody k diagnostike i lecheniyu). Klinicheskie rekomendatsii (protokol lecheniya). Ministerstvo zdravookhraneniya Rossijskoj Federatsii. 2021. Accessed June 08, 2023. (In Russ.). https://moniiag.ru/wp-content/uploads/2019/07/Klinicheskie-rekomendatsii.-Zhenskoe-besplodie.pdf
Recurrent Pregnancy Loss. ESHRE early Pregnancy Guideline Development Group Guideline of European Society of Human Reproduction and Embryology. 2023. Accessed June 08, 2023. https://www.eshre.eu/Guidelines-and-Legal/Guidelines/Recurrent-pregnancy-loss
Aganezov SS, Ponomarenko Kyu, Morotskaya AV, Aganezova NV. Expression of steroid receptors in endometrium within women with desorders in reproductive system. East European Scientific Journal. 2016;12(1):90-93. (In Russ.).
Ponomarenko KYu. Kharakteristika gormon-retseptornogo apparata endometriya u zhenshchin s narusheniyami reproduktivnoj funktsii: Diss. ... kand. med. nauk. SPb. 2018. (In Russ.).
Popova MV, Lutsik VV, Rykova DV, Rykov AA, Zaets EV, Glushich SYu. Thin endometrium as a cause of reproductive losses and unsuccessful IVF attempts (literature review). Mediko-sotsial’nye problemy sem’i. 2020;25(1):86-89. (In Russ.).
Orazov MR, Radzinsky VE, Khamoshina MB, Kaigorodova LA, Toktar LR, Pokul LV, Tulupova MS. Secrets of reproductive failures: «thin» endometrium. Reproduktivnaya meditsina. 2018;2(35):7-17.
Kuzmina AV. Kharakteristika proteomnogo urovnya retseptivnosti endometriya u zhenshchin s narusheniyami reproduktivnoj funktsii: Diss. ... kand. med. nauk. SPb. 2020.
Paulson RJ. Introduction: Endometrial receptivity: evaluation, induction and inhibition. Fertility and Sterility. 2019;111(4):609-610. https://doi.org/10.1016/j.fertnstert.2019.02.029
Fox C, Morin S, Jeong JW, Scott RT Jr, Lessey BA. Local and systemic factors and implantation: what is the evidence? Fertility and Sterility. 2016;105(4):873-884. https://doi.org/10.1016/j.fertnstert.2016.02.018
Zhang J, Sun YF, Xu YM, Shi BJ, Han Y, Luo ZY, Zhao ZM, Hao GM, Gao BL. Effect of endometrium thickness on clinical outcomes in luteal phase short-acting GnRH-a long protocol and GnRH-Ant protocol. Frontiers in Endocrinology. 2021;12:578783. https://doi.org/10.3389/fendo.2021.578783
Melkozerova OA, Bashmakova NV, Esareva AV. Problems of embryo/endometrium communication: Markers of dysfunction and mechanisms of action. Rossijskij vestnik akushera-ginekologa. 2016;16(5):29-36. (In Russ.). https://doi.org/10.17116/rosakush201616529-36
Xi J, Cheng J, Jin CC, Liu JY, Shen ZR, Xia LJ, Li Q, Shen J, Xia YB, Xu B.Electroacupuncture improves pregnancy outcomes in rats with thin endometrium by promoting the expression of pinopode related molecules. BioMed BioMed Research International. 2021;2021: 6658321. https://doi.org/10.1155/2021/6658321
Aganezov SS, Ellinidi VN, Ponomarenko KY, Morotskaya AV, Aganezova NV. Features of the hormone-receptor interaction in the endometrium during ovulatory menstrual cycle in women with reproductive failure. Vestnik Rossijskoj voenno-meditsinskoj akademii. 2018;20(2):63-67. (In Russ.).
Aganezov SS, Ellinidi VN, Morotskaya AV, et al. Endometrial expression of leukemia inhibitory factor in women with different estrogen/progesterone receptor status of the endometrium. Akusherstvo, ginekologiya i reproduktsiya. 2019;13(2):85-94. (In Russ.). https://doi.org/10.17749/2313-7347.2019.13.2.085-094
Leviashvili MM, Mishieva NG, Nazarenko TA, Kogan EA. Leukemia is an inhibitory factor and endometrial receptivity. Problemy Reproduktsii. 2012;3:17-21. (In Russ.).
Sharfi YuZh. Cytokines and growth factors as markers of endometrium implantation capacity in IV. Zhurnal akusherstva i zhenskikh boleznej. 2013;62(4):88-93. (In Russ.).
Cheng JG, Chen JR, Hernandez L, Alvord WG, Stewart CL. Dual control of LIF expression and LIF receptor function regulate Stat3 activation at the onset of uterine receptivity and embryo implantation. Proceedings of the National Academy of Sciences of the United States of America. 2001;(98):8680-8685. https://doi.org/10.1073/pnas.151180898
Aganezova NV, Aganezov SS, Gogichashvili KE. Characteristics of endometrial receptivity in women with different endometrial thickness. Akusherstvo, ginekologiya i reproduktsiya. 2022;16(2):108-121. (In Russ.). https://doi.org/10.17749/2313-7347/ob.gyn.rep.2022.303
Mouhayar Y, Franasiak JM, Sharara FI. Obstetrical complications of thin endometrium in assisted reproductive technologies: a systematic review. Journal of Assisted Reproduction and Genetics. 2019;36(4):607-611. https://doi.org/10.1007/s10815-019-01407-y
Karavani G, Alexandroni H, Sheinin D, Dior UP, Simon A, Ben-Meir A, Reubinoff B. Endometrial thickness following early miscarriage in IVF patients — is there a preferred management approach? Reproductive Biology and Endocrinology. 2021;19(1):93. https://doi.org/10.1186/s12958-021-00780-7
Sundström P. Establishment of a successful pregnancy following in-vitro fertilization with an endometrial thickness of no more than 4 mm. Human Reproduction. 1998;13(6):1550-1552. https://doi.org/10.1093/humrep/13.6.1550
Check JH, Dietterich C, Check ML, Katz Y. Successful delivery despite conception with a maximal endometrial thickness of 4 mm. Clinical and Experimental Obstetrics and Gynecology. 2003;30(2-3): 93-94.