Visualization of the mitotic spindle in oocytes of patients with ovarian endometriosis as a prognostic factor for the effectiveness of fertilization and early embryonic development in IVF/ICSI programs

Krasnopolskaya K.V.; Ershova I.Yu.; Konkova A.L.; Isakova K.M.; Bocharova T.V.

doi:https://doi.org/10.17116/repro20253103155

Krasnopolskaya K.V.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology;
Vladimirsky Moscow Regional Clinical Research Institute

ORCID: 0000-0002-1275-9220

Ershova I.Yu.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9327-0656

Konkova A.L.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

Isakova K.M.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-6194-1654

Bocharova T.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-3747-6067

Visualization of the mitotic spindle in oocytes of patients with ovarian endometriosis as a prognostic factor for the effectiveness of fertilization and early embryonic development in IVF/ICSI programs

Authors:

Krasnopolskaya K.V., Ershova I.Yu., Konkova A.L., Isakova K.M., Bocharova T.V.

More about the authors

Journal: Russian Journal of Human Reproduction. 2025;31(3): 55‑62

DOI: 10.17116/repro20253103155

Read: 457 times

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To cite this article:

Krasnopolskaya KV, Ershova IYu, Konkova AL, Isakova KM, Bocharova TV. Visualization of the mitotic spindle in oocytes of patients with ovarian endometriosis as a prognostic factor for the effectiveness of fertilization and early embryonic development in IVF/ICSI programs. Russian Journal of Human Reproduction. 2025;31(3):55‑62. (In Russ.)
https://doi.org/10.17116/repro20253103155

Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)

Использование инфографики авторами научных работ

MATERIAL AND METHODS

The meiotic spindle presence was studied in 345 MII oocytes of women without endometriosis (the control group), 115 MII oocytes retrieved from unaffected gonads of patients with unilateral ovarian endometriosis (the first comparison group), and 104 oocytes retrieved from affected gonads of patients with unilateral or bilateral ovarian endometriosis (the second comparison group). In selected groups, the reproductive potential of MII oocytes was assessed by their ability to normal fertilization and to form high-quality blastocyst. For visual spindle assessment was used an inverted microscope, equipped with polarization optical system OCTAX PolarAID.

RESULTS

The number of MII oocytes obtained from gonads affected by endometrioses was almost three times less compare to control group (p<0.001), however, oocyte quality assessed by meiotic spindle presence remained unchanged. MII oocytes with meiotic spindle presence derived from both endometriosis-affected and unaffected gonads show normal fertilization 15 percent more often, and provide 10 percent increase of good quality blastocyst rate compare to mature oocytes with no visible spindle.

CONCLUSION

In ovarian endometriosis the number of retrieved mature eggs from the affected gonads significantly reduced, but the quality of MII oocytes assessed in terms of spindle visualization remain uninfluenced. Taking into account the possibility of reproductive potential realization of MII oocytes without visible meiotic spindle it seems reasonable to perform their fertilization using ICSI method, especially in stimulated cycles with poor response.

Keywords:

Infertility

ovarian endometriosis

meiotic spindle

oocyte fertilization

blastocysts

IVF/ICSI

Authors:

Krasnopolskaya K.V.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology;
Vladimirsky Moscow Regional Clinical Research Institute

ORCID: 0000-0002-1275-9220

Ershova I.Yu.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9327-0656

Konkova A.L.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

Isakova K.M.

Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-6194-1654

Bocharova T.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-3747-6067

Received:

09.01.2024

Accepted:

29.05.2024

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References:

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