The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.
Shiryaev A.A.
Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Kalyagina N.A.
Prokhorov General Physics Institute;
National Research Nuclear University
Efendiev K.T.
Prokhorov General Physics Institute;
National Research Nuclear University
Reshetov I.V.
I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of Russia
Udeneev A.M.
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Loshchenov V.B.
Prokhorov General Physics Institute;
National Research Nuclear University
Photodynamic inactivation of antibiotic-resistant microflora of gunshot wounds under fluorescence control
Journal: Pirogov Russian Journal of Surgery. 2025;(2): 50‑59
Views: 446
Downloaded: 6
To cite this article:
Shiryaev AA, Ivankov MP, Kalyagina NA, et al. . Photodynamic inactivation of antibiotic-resistant microflora of gunshot wounds under fluorescence control. Pirogov Russian Journal of Surgery.
2025;(2):50‑59. (In Russ.)
https://doi.org/10.17116/hirurgia202502150
Antimicrobial photodynamic therapy or photodynamic inactivation (PDI) of antibiotic-resistant microflora seems to be a very promising alternative, including for the treatment of long-term non-healing wounds, due to its potential and extremely low possibility of resistance.
The authors describe an effective approach to preparing the wounds for delayed surgical treatment (secondary suturing, split-flap autodermoplasty, wound defect closure with local tissues), as well as healing by secondary intention of wounds using PDI of antibiotic-resistant microflora under spectroscopic control of fluorescence intensity in tissues. For this purpose, laser and LED radiation with wavelengths 660—680 nm was used. Various photosensitizers (methylene blue, aluminum phthalocyanine, chlorin e6 and their emulsion forms) were used. The study included 90 patients with various wounds.
Positive treatment outcomes were obtained in most patients. The first PDI procedure decreased concentration of microflora by 3—4 times. There was significant or complete inactivation of bacteria by the end of the treatment. In all patients, concentration of photosensitizers significantly decreased by more than 75% after PDI. Surgical treatment was successful in 100% of cases (n=56; 62%).
PDI is a promising method for inactivating antibiotic-resistant microflora, including patients with long-term non-healing wounds. This method contributes to safe and high-quality surgical treatment. A great advantage of PDI is its multi-purpose mechanism and no resistance of microorganisms.
Keywords:
Authors:
Shiryaev A.A.
Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation (Sechenov University)
Kalyagina N.A.
Prokhorov General Physics Institute;
National Research Nuclear University
Efendiev K.T.
Prokhorov General Physics Institute;
National Research Nuclear University
Reshetov I.V.
I.M. Sechenov First Moscow State Medical University (Sechenov University) of the Ministry of Health of Russia
Udeneev A.M.
National Research Nuclear University MEPhI (Moscow Engineering Physics Institute)
Loshchenov V.B.
Prokhorov General Physics Institute;
National Research Nuclear University
Received:
20.10.2024
Accepted:
07.11.2024
List of references:
Close metadata
Email Confirmation
An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.
Email Confirmation
Log in to the site using your account in one of the services
We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.