Experimental substantiation of medical device design for mechanical processing of adipose tissue

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OBJECTIVE

To substantiate the medical device design for mechanical processing of adipose tissue.

MATERIAL AND METHODS

Enzymatic method using collagenase and mechanical method for obtaining the stromal vascular fraction (SVF) are compared. The authors emphasized the disadvantages of enzymatic method, such as high cost and duration of process. The main criteria for developing the design were cell viability after processing of adipose tissue and available processing in a minimum number of passages manually without automated mechanical stands.

RESULTS

The proposed design allows two unidirectional passages to obtain processed lipoaspirate for SVF harvesting. Experimental studies demonstrate that meshes with a minimum cell size of 100 μm allow high cell viability comparable to enzymatic method. Moreover, this design can be used to develop a disposable medical device.

CONCLUSION

Our results will underlie development of a disposable medical device for mechanical processing of adipose tissue.

Keywords:

stromal vascular fraction

adipose tissue

regenerative medicine

stromal vascular fraction extraction system

SVF

medical device

Authors:

Eremin I.I.

Petrovsky National Research Center of Surgery

ORCID: 0000-0002-7284-5649

Vasiliev V.S.

Institute of Plastic Surgery and Cosmetology

ORCID: 0000-0003-2752-8836

Tikhomirov A.N.

Bauman Moscow State Technical University

ORCID: 0000-0002-5989-9044

Chibizov P.E.

Bauman Moscow State Technical University

ORCID: 0009-0003-9379-0629

Briko A.N.

Petrovsky National Research Center of Surgery

ORCID: 0000-0002-7284-5649

Kotenko K.V.

Petrovsky National Research Center of Surgery

ORCID: 0000-0002-6147-5574

Received:

13.08.2024

Accepted:

21.09.2024

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