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Vlasov A.P.

National Research Mordovia State University

Brodovskaya E.P.

National Research Mordovia State University

Madonov K.S.

National Research Mordovia State University

Abelova A.P.

National Research Mordovia State University

Bezborodova A.P.

National Research Mordovia State University

Pleshkov S.A.

National Research Mordovia State University

Vlasova T.I.

National Research Mordovia State University

Impaired reparative regeneration in patients with acute appendicitis

Authors:

Vlasov A.P., Brodovskaya E.P., Madonov K.S., Abelova A.P., Bezborodova A.P., Pleshkov S.A., Vlasova T.I.

More about the authors

Journal: Pirogov Russian Journal of Surgery. 2026;(2): 73‑84

DOI: 10.17116/hirurgia202602173

Read: 660 times

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Table of contents

IMPAIRED REPARATIVE REGENERATION IN PATIENTS WITH ACUTE APPENDICITIS
IMPAIRED REPARATIVE REGENERATION IN PATIENTS WITH ACUTE APPENDICITIS

To cite this article:

Vlasov AP, Brodovskaya EP, Madonov KS, Abelova AP, Bezborodova AP, Pleshkov SA, Vlasova TI. Impaired reparative regeneration in patients with acute appendicitis. Pirogov Russian Journal of Surgery. 2026;(2):73‑84. (In Russ.)
https://doi.org/10.17116/hirurgia202602173

Подписка 2026 Хирургия. Журнал им. Н.И. Пирогова (Pirogov Russian Journal of Surgery)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

To study reparative potential (cellular effects and biological characteristics) of platelet-rich plasma (PRP) in patients with acute appendicitis (AA).

MATERIAL AND METHODS

PRP was obtained by two-stage centrifugation of blood from patients with AA and healthy donors divided into age groups: group 1 (n=10) — healthy donors aged 30—40 years, group 2 (n=10) — healthy donors aged 60—70 years, group 3 (n=10) — patients with AA aged 30—40 years, group 4 (n=10) — patients with AA aged 60—70 years. The study was performed using hTERT-HDFa (d220) cell line of human dermal fibroblasts (HDF). Activated 10% PRP from donors was added to experimental wells. Samples without PRP were negative control (0 (K)), PRP samples with addition of 10% FBS — positive control. Metabolic activity of cells, viability of samples, ability to generate reactive oxygen species (ROS), migration activity and intensity of cellular aging were assessed. Biological characteristics of PRP were defined by ELISA of IL6, IL10, TGFB1, PAI-1, PDGF, VEGF proteins.

RESULTS

PRP from elderly patients with AA was characterized by elevated IL6 and IL10 relative to young patients with this pathology and increased TGFβ relative to healthy peers. On the first day after HDF stimulation with plasma, the lowest viability was found in samples stimulated with PRP from health elderly donors. There were no significant differences among patients with AA regardless of age. After 3 days, we observed high proliferative and metabolic activity of cells after stimulation with plasma from AA patients regardless of age. Antioxidant reserve of PRP in elderly patients with AA was significantly lower than in young patients with AA. Correlation analysis revealed significant relationships between biological profile of PRP, cellular responses and clinical data of patients with AA of different ages.

CONCLUSION

When comparing cellular responses with clinical data of patients, we found that significant prognostic markers of PRP in AA were IL6, IL10 and TGFβ. Their levels correlated with postoperative hospital-stay. Moreover, high IL6 and IL10 was associated with prolonged hospital-stay in elderly patients.

Keywords:

platelet-rich plasma
regenerative medicine
personalized medicine
patient age
acute appendicitis
human dermal fibroblasts
inflammation

Authors:

Vlasov A.P.

National Research Mordovia State University

Brodovskaya E.P.

National Research Mordovia State University

Madonov K.S.

National Research Mordovia State University

Abelova A.P.

National Research Mordovia State University

Bezborodova A.P.

National Research Mordovia State University

Pleshkov S.A.

National Research Mordovia State University

Vlasova T.I.

National Research Mordovia State University

Received:

13.08.2025

Accepted:

14.09.2025

Close metadata

References:

  1. Yamada S, Behfar A, Terzic A. Regenerative Medicine Clinical Readiness. Regen Med. 2021;16(3):309-322.  https://doi.org/10.2217/rme-2020-0178
  2. Terzic A, Pfenning MA, Gores GJ, Harper CM Jr. Regenerative Medicine Build-Out. Stem Cells Transl Med. 2015;4(12):1373-1379. https://doi.org/10.5966/sctm.2015-0275
  3. Singer AJ. Healing Mechanisms in Cutaneous Wounds: Tipping the Balance. Tissue Eng Part B Rev. 2022;28(5):1151-1167. https://doi.org/10.1089/ten.TEB.2021.0114
  4. Karin M, Clevers H. Reparative inflammation takes charge of tissue regeneration. Nature. 2016;529(7586):307-315.  https://doi.org/10.1038/nature17039
  5. Yushkov B.G. Immune system and regulation of regeneration. Bulletin of Siberian Medicine. 2017;16(4):94-105. (In Russ.). https://doi.org/10.20538/1682-0363-2017-4-94-105
  6. Martin P, Nunan R. Cellular and molecular mechanisms of repair in acute and chronic wound healing. Br J Dermatol. 2015;173(2):370-378.  https://doi.org/10.1111/bjd.13954
  7. Vlasova TI, Brodovskaya EP, Madonov KS, Ageev VP, Abelova AP, Pinyaev SI, Vlasov AP. Regenerative Potential of Platelet-Rich Plasma in Diabets Mellitus. Dokl Biol Sci. 2025;521(1):123-133.  https://doi.org/10.1134/S0012496624600556
  8. Vlasova TI, Brodovskaya EP, Vlasov AP, Madonov KS, Kovalenko EN, Myakushin SS, Polozova AI. Regenerative capabilities of platelet-rich plasma in different age groups. Pirogov Russian Journal of Surgery. 2025;5:36-43. (In Russ.). https://doi.org/10.17116/hirurgia202505136
  9. Barabanshchikov AA, Gorelik SG, Miliutina EV, Ishchenko KA. To identify differences in the postoperative period in patients of different age groups and surgical profile. Innova. 2020;3:14-17. (In Russ.).
  10. Odložilová Š, Paral J, Sirovy M, Zajak J, Fibír A. Platelet-Rich Plasma: Characteristics and Current Review of its Use in Surgery. Pol Przegl Chir. 2024;96(5):66-74.  https://doi.org/10.5604/01.3001.0054.6750
  11. Xu J, Gou L, Zhang P, Li H, Qiu S. Platelet-rich plasma and regenerative dentistry. Aust Dent J. 2020;65(2):131-142.  https://doi.org/10.1111/adj.12754
  12. Phoebe LKW, Lee KWA, Chan LKW, Hung LC, Wu R, Wong S, Wan J, Yi KH. Use of platelet rich plasma for skin rejuvenation. Skin Res Technol. 2024;30(4):13714. https://doi.org/10.1111/srt.13714
  13. Zhu L, Li P, Qin Y, Xiao B, Li J, Xu W, Yu B. Platelet-rich plasma in orthopedics: Bridging innovation and clinical applications for bone repair. J Orthop Surg (Hong Kong). 2024;32(1):10225536231224952. https://doi.org/10.1177/10225536231224952
  14. Dhurat R, Sukesh M. Principles and Methods of Preparation of Platelet-Rich Plasma: A Review and Author’s Perspective. J Cutan Aesthet Surg. 2014;7(4):189-197.  https://doi.org/10.4103/0974-2077.150734
  15. Delgado D, Bilbao AM, Beitia M, Garate A, Sánchez P, González-Burguera I, Isasti A, López De Jesús M, Zuazo-Ibarra J, Montilla A, Domercq M, Capetillo-Zarate E, García Del Caño G, Sallés J, Matute C, Sánchez M. Effects of Platelet-Rich Plasma on Cellular Populations of the Central Nervous System: The Influence of Donor Age. Int J Mol Sci. 2021;22(4):1725. https://doi.org/10.3390/ijms22041725
  16. Niemann M, Ort M, Lauterbach L, Streitz M, Wilhelm A, Grütz G, Fleckenstein FN, Graef F, Blankenstein A, Reinke S, Stöckle U, Perka C, Duda GN, Geißler S, Winkler T, Maleitzke T. Individual immune cell and cytokine profiles determine platelet-rich plasma composition. Arthritis Res Ther. 2023;25(1):6.  https://doi.org/10.1186/s13075-022-02969-6
  17. https://diseases.medelement.com/disease/%D0%BE%D1%81%D1%82%D1%80%D1%8B%D0%B9-%D0%B0%D0%BF%D0%BF%D0%B5%D0%BD%D0%B4%D0%B8%D1%86%D0%B8%D1%82-%D1%83-%D0%B2%D0%B7%D1%80%D0%BE%D1%81%D0%BB%D1%8B%D1%85-%D0%BA%D1%80-%D1%80%D1%84-2023/17774?ysclid=me66104hdz483416606 
  18. Segev L, Keidar A, Schrier I, Rayman S, Wasserberg N, Sadot E. Acute appendicitis in the elderly in the twenty-first century. J Gastrointest Surg. 2015;19(4):730-735.  https://doi.org/10.1007/s11605-014-2716-9
  19. Kotaluoto S, Ukkonen M, Pauniaho SL, Helminen M, Sand J, Rantanen T. Mortality Related to Appendectomy; a Population Based Analysis over Two Decades in Finland. World J Surg. 2017;41(1):64-69.  https://doi.org/10.1007/s00268-016-3688-6
  20. Fugazzola P, Ceresoli M, Agnoletti V, Agresta F, Amato B, Carcoforo P, Catena F, Chiara O, Chiarugi M, Cobianchi L, Coccolini F, De Troia A, Di Saverio S, Fabbri A, Feo C, Gabrielli F, Gurrado A, Guttadauro A, Leone L, Marrelli D, Petruzzelli L, Portolani N, Prete FP, Puzziello A, Sartelli M, Soliani G, Testini M, Tolone S, Tomasoni M, Tugnoli G, Viale P, Zese M, Ishay OB, Kluger Y, Kirkpatrick A, Ansaloni L. The SIFIPAC/WSES/SICG/SIMEU guidelines for diagnosis and treatment of acute appendicitis in the elderly (2019 edition). World J Emerg Surg. 2020;15(1):19.  https://doi.org/10.1186/s13017-020-00298-0
  21. Bhullar JS, Chaudhary S, Cozacov Y, Lopez P, Mittal VK. Acute appendicitis in the elderly: diagnosis and management still a challenge. Am Surg. 2014;80(11):295-297. 
  22. Kon E, Di Matteo B, Delgado D, Cole BJ, Dorotei A, Dragoo JL, Filardo G, Fortier LA, Giuffrida A, Jo CH, Magalon J, Malanga GA, Mishra A, Nakamura N, Rodeo SA, Sampson S, Sánchez M. Platelet-rich plasma for the treatment of knee osteoarthritis: an expert opinion and proposal for a novel classification and coding system. Expert Opin Biol Ther. 2020;20(12):1447-1460. https://doi.org/10.1080/14712598.2020.1798925
  23. Xu S, Deng KQ, Lu C, Fu X, Zhu Q, Wan S, Zhang L, Huang Y, Nie L, Cai H, Wang Q, Zeng H, Zhang Y, Wang F, Ren H, Chen Y, Yan H, Xu K, Zhou L, Lu M, Zhu Y, Liu S, Lu Z. Interleukin-6 classic and trans-signaling utilize glucose metabolism reprogramming to achieve anti- or pro-inflammatory effects. Metabolism. 2024;155:155832. https://doi.org/10.1016/j.metabol.2024.155832
  24. Shaikh SB, Balaya RDA, Dagamajalu S, Bhandary YP, Unwalla H, Prasad TSK, Rahman I. A signaling pathway map of plasminogen activator inhibitor 1 (PAI-1/SERPINE-1): a review of an innovative frontier in molecular aging and cellular senescence. Cell Commun Signal. 2024;22(1):544.  https://doi.org/10.1186/s12964-024-01910-5
  25. Morrow GB, Whyte CS, Mutch NJ. A Serpin With a Finger in Many PAIs: PAI-1’s Central Function in Thromboinflammation and Cardiovascular Disease. Front Cardiovasc Med. 2021;8:653655. https://doi.org/10.3389/fcvm.2021.653655
  26. Qin Y, Zhang J, Babapoor-Farrokhran S, Applewhite B, Deshpande M, Megarity H, Flores-Bellver M, Aparicio-Domingo S, Ma T, Rui Y, Tzeng SY, Green JJ, Canto-Soler MV, Montaner S, Sodhi A. PAI-1 is a vascular cell—specific HIF-2—dependent angiogenic factor that promotes retinal neovascularization in diabetic patients. Sci Adv. 2022;8(9):eabm1896. https://doi.org/10.1126/sciadv.abm1896
  27. Abdullah FE, Ahmed SN. Serum lipoprotein(a), and plasminogen activator inhibitor-1 in uncomplicated type 2 diabetes mellitus: a case-control study. Ann Med Surg (Lond). 2023;85(8):3801-3805. https://doi.org/10.1097/MS9.0000000000000915
  28. Melincovici CS, Boşca AB, Şuşman S, Mărginean M, Mihu C, Istrate M, Moldovan IM, Roman AL, Mihu CM. Vascular endothelial growth factor (VEGF) — key factor in normal and pathological angiogenesis. Rom J Morphol Embryol. 2018;59(2):455-467. 
  29. Morikawa M, Derynck R, Miyazono K. TGF-β and the TGF-β Family: Context-Dependent Roles in Cell and Tissue Physiology. Cold Spring Harb Perspect Biol. 2016;8(5):a021873. https://doi.org/10.1101/cshperspect.a021873
  30. Heldin CH, Westermark B. Mechanism of action and in vivo role of platelet-derived growth factor. Physiol Rev. 1999;79(4):1283-1316. https://doi.org/10.1152/physrev.1999.79.4.1283
  31. Wang X, Wong K, Ouyang W, Rutz S. Targeting IL-10 Family Cytokines for the Treatment of Human Diseases. Cold Spring Harb Perspect Biol. 2019;11(2):a028548. https://doi.org/10.1101/cshperspect.a028548
  32. Hannoodee S, Nasuruddin DN. Acute Inflamm Response. Treasure Island (FL): StatPearls Publishing; 2025.
  33. Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. Inflammatory responses and inflammation-associated diseases in organs. Oncotarget. 2017;9(6):7204-7218. https://doi.org/10.18632/oncotarget.23208
  34. Nailwal NP, Doshi GM. Role of intracellular signaling pathways and their inhibitors in the treatment of inflammation. Inflammopharmacology. 2021;29(3):617-640.  https://doi.org/10.1007/s10787-021-00813-y
  35. Hajam YA, Rani R, Ganie SY, Sheikh TA, Javaid D, Qadri SS, Pramodh S, Alsulimani A, Alkhanani MF, Harakeh S, Hussain A, Haque S, Reshi MS. Oxidative Stress in Human Pathology and Aging: Molecular Mechanisms and Perspectives. Cells. 2022;11(3):552.  https://doi.org/10.3390/cells11030552
  36. Arulselvan P, Fard MT, Tan WS, Gothai S, Fakurazi S, Norhaizan ME, Kumar SS. Role of Antioxidants and Natural Products in Inflammation. Oxid Med Cell Longev. 2016;2016:5276130. https://doi.org/10.1155/2016/5276130
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