Microbiota markers level in the blood and cerebrospinal fluid of patients with different types of multiple sclerosis and radiologically isolated syndrome

Omarova M.A.

Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency;
Pirogov Russian National Research Medical University

ORCID: 0000-0002-6744-2191

Rogovskii V.S.

Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency;
Pirogov Russian National Research Medical University

ORCID: 0000-0002-3682-6571

Sadekov T.Sh.

Gabrichevsky Research Institute for Epidemiology and Microbiology

ORCID: 0000-0001-5337-0054

Sadekova G.I.

Pirogov Russian National Research Medical University

ORCID: 0000-0002-4123-2313

Zhilenkova O.G.

Gabrichevsky Research Institute for Epidemiology and Microbiology

ORCID: 0000-0003-3206-6648

Boyko A.N.

Pirogov Russian National Research Medical University;
Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency

SPIN RSCI: 9921-9109
Scopus AuthorID: 7102247663
ORCID: 0000-0002-2975-4151

Microbiota markers level in the blood and cerebrospinal fluid of patients with different types of multiple sclerosis and radiologically isolated syndrome

Authors:

Omarova M.A., Rogovskii V.S., Sadekov T.Sh., Sadekova G.I., Zhilenkova O.G., Boyko A.N.

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Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2023;123(7‑2): 96‑102

DOI: 10.17116/jnevro202312307296

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To cite this article:

Omarova MA, Rogovskii VS, Sadekov TSh, Sadekova GI, Zhilenkova OG, Boyko AN. Microbiota markers level in the blood and cerebrospinal fluid of patients with different types of multiple sclerosis and radiologically isolated syndrome. S.S. Korsakov Journal of Neurology and Psychiatry. 2023;123(7‑2):96‑102. (In Russ.)
https://doi.org/10.17116/jnevro202312307296

Подписка 2025-2 (S.S. Korsakov Journal of Neurology and Psychiatry (special issues))

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OBJECTIVE

To assess the level of microbiota markers in the blood and cerebrospinal fluid (CSF) of patients with different types of multiple sclerosis (MS), people with radiologically isolated syndrome (RIS) and control subjects.

MATERIAL AND METHODS

We used gas chromatography-mass spectrometry (GC-MS) to evaluate the levels of microbiota markers in 69 patients with different types of MS (27 patients in the acute stage, 35 patients with MS in remission, 7 patients with primary-progressive MS), 10 people with RIS, and 47 control subjects (different diseases of the nervous system of a non-autoimmune or inflammatory nature).

RESULTS

We showed a statistically significant increase in the content of various microbiota markers in the CSF of patients with MS compared with the control group. We found no change in the content of these markers in blood of patients with MS. This suggests a change of markers of microbial load at the level of the central nervous system, but not at the level of the whole organism. The greatest number of statistically significant differences with the control group was found in the content of markers in CSF of patients with MS in remission. In the acute stage, on the contrary, we found no statistically significant differences compared to the control group. In particular, in CSF of patients with MS in remission, a statistically significant increase in the content of bacterial plasmalogen (4.5 times), and increase in the level of microbial markers specific to Peptostreptococcus anaerobius, Pseudomonas aeruginosa, Eubacterium, Bifidobacterium, Butirivibrio, Moraxella, Acinetobacter, Propionibacterium acnes, as well as an increase of markers of the Epstein-Barr virus were found. In addition, there was an increase of campesterol, the likely source of which is campesterol-producing microfungi. In the CSF of subjects with RIS there were a statistically significant increase in the level of markers of the Epstein-Barr virus, Propionibacterium acnes, as well as Pseudomonas, Moraxella, and Acinetobacter.

CONCLUSION

An association of MS with polymicrobial infection is possible. It is also likely that there is a certain pattern of increase of microbiota markers in the CSF of patients with MS, but not in blood.

Keywords:

multiple sclerosis

microbiota

polymicrobial infection

microbiotic markers

cerebrospinal fluid

Authors:

Omarova M.A.

Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency;
Pirogov Russian National Research Medical University

ORCID: 0000-0002-6744-2191

Rogovskii V.S.

Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency;
Pirogov Russian National Research Medical University

ORCID: 0000-0002-3682-6571

Sadekov T.Sh.

Gabrichevsky Research Institute for Epidemiology and Microbiology

ORCID: 0000-0001-5337-0054

Sadekova G.I.

Pirogov Russian National Research Medical University

ORCID: 0000-0002-4123-2313

Zhilenkova O.G.

Gabrichevsky Research Institute for Epidemiology and Microbiology

ORCID: 0000-0003-3206-6648

Boyko A.N.

Pirogov Russian National Research Medical University;
Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency

SPIN RSCI: 9921-9109
Scopus AuthorID: 7102247663
ORCID: 0000-0002-2975-4151

Received:

17.05.2023

Accepted:

26.05.2023

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