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Baulina N.M.

National Medical Research Center of Cardiology;
Pirogov Russian National Research Medical University

Kabaeva A.R.

Pirogov Russian National Research Medical University

Boyko A.N.

Pirogov Russian National Research Medical University;
Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency

Favorova O.O.

Pirogov Russian National Research Medical University

Expression analysis of miRNAs from the DLK1-DIO3 locus in CD4+ and CD14+ cells in patients with relapsing-remitting multiple sclerosis

Authors:

Baulina N.M., Kabaeva A.R., Boyko A.N., Favorova O.O.

More about the authors

Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2022;122(7‑2): 52‑59

DOI: 10.17116/jnevro202212207252

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Table of contents

EXPRESSION ANALYSIS OF MIRNAS FROM THE DLK1-DIO3 LOCUS IN CD4+ AND CD14+ CELLS IN PATIENTS WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS
EXPRESSION ANALYSIS OF MIRNAS FROM THE DLK1-DIO3 LOCUS IN CD4+ AND CD14+ CELLS IN PATIENTS WITH RELAPSING-REMITTING MULTIPLE SCLEROSIS

To cite this article:

Baulina NM, Kabaeva AR, Boyko AN, Favorova OO. Expression analysis of miRNAs from the DLK1-DIO3 locus in CD4+ and CD14+ cells in patients with relapsing-remitting multiple sclerosis. S.S. Korsakov Journal of Neurology and Psychiatry. 2022;122(7‑2):52‑59. (In Russ.)
https://doi.org/10.17116/jnevro202212207252

Подписка 2026 Журнал неврологии и психиатрии им. С.С. Корсакова. Спецвыпуски (S.S. Korsakov Journal of Neurology and Psychiatry (special issues))
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Abstract:

OBJECTIVE

To analyze the expression of several previously unstudied miRNAs from the DLK1-DIO3 locus in peripheral blood mononuclear cells (PBMC) and in CD14+ and CD4+ cell subpopulations in patients with relapsing-remitting multiple sclerosis (RRMS) and healthy individuals.

MATERIAL AND METHODS

MiRNA expression analysis was performed in PBMC, CD14+, and CD4+ cells of 14 patients who did not take immunomodulatory drugs, and 14 individuals without autoimmune and inflammatory diseases by reverse transcription followed by real-time PCR.

RESULTS

Expression levels of 10 miRNAs selected based on different criteria were significantly higher in PBMC in men with RRMS compared to healthy men; in women, their expression did not change in a similar comparison. No mass unidirectional changes in the expression of these miRNAs in RRMS men were observed in CD14+ and CD4+ cells. High consistency in the expression of miRNA pairs was also not found.

CONCLUSIONS

The analysis of PBMC showed the involvement of 10 more miRNAs encoded by genes from the DLK1-DIO3 locus in the development of RRMS. The main observed effect of the increase in the expression levels of these miRNAs in men with RRMS compared with healthy men is not achieved due to the contribution of CD14+ and CD4+ cells. This opens up possibilities for studying the involvement of miRNAs in other PBMC subpopulations in the pathological processes in RRMS.

Keywords:

multiple sclerosis
miRNA
imprinted DLK1-DIO3 locus
CD4+ cells
CD14+ cells

Authors:

Baulina N.M.

National Medical Research Center of Cardiology;
Pirogov Russian National Research Medical University

Kabaeva A.R.

Pirogov Russian National Research Medical University

Boyko A.N.

Pirogov Russian National Research Medical University;
Federal Center of Brain Research and Neurotechnologies of the Federal Medical Biological Agency

Favorova O.O.

Pirogov Russian National Research Medical University

Received:

09.05.2022

Accepted:

18.05.2022

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References:

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  2. GBD 2016 Neurology Collaborators. Global, regional, and national burden of neurological disorders, 1990—2016: a systematic analysis for the Global Burden of Disease Study. The Lancet Neurol. 2019;18(5):459-480.  https://doi.org/10.1016/S1474-4422(18)30499-X
  3. Sadovnick A, Yee I, Ebers G and Canadian Collaborative Study Group. Factors influencing sib risks for multiple sclerosis. Clinical genetics. 2020;58(6):431-435.  https://doi.org/10.1034/j.1399-0004.2000.580602.x
  4. Ortona E, Delunardo F, Baggio G, Malorni W. A sex and gender perspective in medicine: a new mandatory challenge for human health. Ann Ist Super Sanita. 2016;52(2):146-148.  https://doi.org/10.4415/ANN_16_02_02
  5. Baulina N, Osmak G, Kiselev I, et al. MiRNAs from DLK1-DIO3 Imprinted Locus at 14q32 are Associated with Multiple Sclerosis: Gender-Specific Expression and Regulation of Receptor Tyrosine Kinases Signaling. Cells. 2019;8(2):133-140.  https://doi.org/10.3390/cells8020133
  6. Thompson AJ, Banwell BL, Barkhof F, et al. Diagnosis of multiple sclerosis: 2017 revisions of the McDonald criteria. The Lancet. Neurology. 2018;17(2):162-173.  https://doi.org/10.1016/S1474-4422(17)30470-2
  7. Goossens EAC, de Vries MR, Simons KH, et al. miRMap: Profiling 14q32 microRNA Expression and DNA Methylation Throughout the Human Vasculature. Front Cardiovasc Med. 2019;6:113.  https://doi.org/10.3389/fcvm.2019.00113
  8. García-López S, Albo-Castellanos C, Urdinguio RG, et al. Deregulation of the imprinted DLK1-DIO3 locus ncRNAs is associated with replicative senescence of human adipose-derived stem cells. PLoS One. 2018;13(11):e0206534. https://doi.org/10.1371/journal.pone.0206534
  9. Liko D, Rzepiela A, Vukojevic V, et al. Loss of TSC complex enhances gluconeogenesis via upregulation of Dlk1-Dio3 locus miRNAs. Proc Natl Acad Sci U S A. 2020;117(3):1524-1532. https://doi.org/10.1073/pnas.1918931117
  10. Zhang J, Li H, Dong J. Omics-Based Identification of Shared and Gender Disparity Routes in Hras12V-Induced Hepatocarcinogenesis: An Important Role for Dlk1-Dio3 Genomic Imprinting Region. Front Genet. 2021;12:620594. https://doi.org/10.3389/fgene.2021.620594
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