The dependence of the ploidic status of embryos on the state of the spindle of oocyte division, assessed using polarization microscopy in IVF programs in women with ovarian endometriosis

Krasnopol’skaya K.V.; Ershova I.Yu.; Kon’kova A.L.; Samoylova A.A.; Bocharova T.V.

doi:https://doi.org/10.17116/rosakush20252501144

Krasnopol’skaya K.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology;
M.F. Vladimirsky Moscow Regional Scientific Research Clinical Institute

ORCID: 0000-0002-1275-9220

Ershova I.Yu.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9327-0656

Kon’kova A.L.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9910-7542

Samoylova A.A.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-2059-6425

Bocharova T.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-3747-6067

The dependence of the ploidic status of embryos on the state of the spindle of oocyte division, assessed using polarization microscopy in IVF programs in women with ovarian endometriosis

Authors:

Krasnopol’skaya K.V., Ershova I.Yu., Kon’kova A.L., Samoylova A.A., Bocharova T.V.

More about the authors

Journal: Russian Bulletin of Obstetrician-Gynecologist. 2025;25(1): 44‑49

DOI: 10.17116/rosakush20252501144

Read: 870 times

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To cite this article:

Krasnopol’skaya KV, Ershova IYu, Kon’kova AL, Samoylova AA, Bocharova TV. The dependence of the ploidic status of embryos on the state of the spindle of oocyte division, assessed using polarization microscopy in IVF programs in women with ovarian endometriosis. Russian Bulletin of Obstetrician-Gynecologist. 2025;25(1):44‑49. (In Russ.)
https://doi.org/10.17116/rosakush20252501144

Подписка 2026 Российский вестник акушера-гинеколога (Russian Bulletin of Obstetrician-Gynecologist)

Использование инфографики авторами научных работ

OBJECTIVE

In infertile women with and without ovarian endometriosis, according to preimplantation genetic testing for aneuploidy, the ploidic status of embryos generated from mature oocytes (MII oocytes) with and without a visually detectable division spindle should be clarified.

MATERIAL AND METHODS

Using the polarizing optical system OCTAX PolarAID, the state of the division spindle was studied in 345 mature oocytes of women younger than 36 years with healthy gonads (control group) and in 219 mature oocytes of women of the same age suffering from ovarian endometriosis (main group). The influence of the presence/absence of a division spindle in MII oocytes on the frequency of their correct fertilization, blastulation and the ploidic status of the resulting embryos was evaluated. Preimplantation genetic testing for aneuploidy was performed by Next Generation Sequencing (NGS).

RESULTS

In the cohort of blastocysts that were obtained from MII oocytes with an unvisualized fission spindle, the frequency of embryonic aneuploidy was 2—3 times higher than among embryos generated from mature oocytes with a normal fission spindle state. This pattern was reliable and manifested itself to the same extent in women with and without ovarian endometriosis.

CONCLUSION

When performing the embryological stage of IVF programs in women with ovarian endometriosis and the normal state of the gonads, it is advisable to clarify, using polarization microscopy, the presence of a division spindle in all isolated adult oocytes and subsequently check for aneuploidy using pre-implantation genetic testing, all blastocysts generated from eggs with a visually undefined division spindle.

Keywords:

infertility

ovarian endometriosis

IVF

spindle division

preimplantation genetic testing for aneuploidy

Authors:

Krasnopol’skaya K.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology;
M.F. Vladimirsky Moscow Regional Scientific Research Clinical Institute

ORCID: 0000-0002-1275-9220

Ershova I.Yu.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9327-0656

Kon’kova A.L.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0001-9910-7542

Samoylova A.A.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-2059-6425

Bocharova T.V.

V.I. Krasnopolsky Moscow Regional Research Institute of Obstetrics and Gynecology

ORCID: 0000-0002-3747-6067

Received:

21.05.2024

Accepted:

19.06.2024

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References:

Webster A, Schuh M. Mechanism of aneuploidy in human eggs. Trends Cell Biol. 2017;27:1:55-68. https://doi.org/10.1016/j.tcb.2016.09.002
Gardner RJM, Suitherland HR. Chromosome abnormalities and genetic counseling. Oxford Monographs on Medical Genetics. Oxford University Press; 5th edition 2018.
Schmutzler AG. Theory and practice of preimplantation genetic screening (PGS). J Med Genet. 2019;62:8:103670. https://doi.org/10.1016/j.ejmg.2019.103670
Kahraman S, Sahin Y, Yelke H, Kumtepe Y, Tufekci MA, Yapan CC, Yesil M, Cetinkaya M. High rates of aneuploidy, mosaicism and abnormal morphokinetic development in cases with low sperm concentration. J Assist Reprod Genet. 2020;37:3:629-640. https://doi.org/10.1007/s10815-019-01673-w
Sato T, Sugiura-Ogasawara M, Ozawa F, Yamamoto T, Kato T, Kurahashi H, Kuroda T, Aoyama N, Kato K, Kobayashi R, Fukuda A, Utsunomiya T, Kuwahara A, Saito H, Takeshita T, Irahara M. Preimpantation genetic testing for aneuploidy: a comparison of live birth rates in patients with recurrent pregnancy loss due to embryonic aneuploidy of recurrent implantation failure. Hum Reprod. 2019;34:12:12340-2348. https://doi.org/10.1093/humrep/dez229
Marin D, Xu J, Treff NR. Preimpantation genetic testing for aneuploidy: A review of published blastocyst reanalysis concordance data. Prenatal Diagn. 2021;41:5:545-553. https://doi.org/10.1002/pd.5828
Sermon K, Capalbo A, Cohen J, Coonen E, De Rycke M, De Vos A, Delhanty J, Fiorentino F, Gleicher N, Griesinger G, Grifo J, Handyside A, Harper J, Kokkali G, Mastenbroek S, Meldrum D, Meseguer M, Montag M., Munné S, Rienzi L, Rubio C, Scott K, Scott R, Simon C, Swain J, Treff T, Ubaldi F, Vassena R, Vermeesch JR, Verpoest W, Wells D, Geraedts J. The why, the how and the when of PGS 2.0: current practices and expert opinions of fertility specialists, molecular biologists, and embryologists. Mol Hum Reprod. 2016;22:8:845-857. https://doi.org/10.1093/molehr/gaw034
Practice Committees of the American Society for Reproductive Medicine and the Society for Assisted Reproductive Technology. Electronic address: https://doi.org/10.1016/j.fertnstert.2018.01.002
Korsak VS, Smirnova AA, Shurygina OV. ART Register of RAHR, 2020. Russian Journal of Human Reproduction. 2022;28:6:12-27. (In Russ.). https://doi.org/10.17116/repro20222806112
Gardner D, Schoolcraft W, Wagley L, Schlenker T, Stevens J, Hesla J. A prospective randomized trial of blastocyst culture and transfer in IVF. Hum Reprod. 1998;13:12:3434-3440. https://doi.org/10.1093/humrep/13.12.3434
Krasnopol’skaya KV. Treatment of infertility with endometriosis. A reproductive specialist’s view. Moscow: MEDpress-inform. 2019. (In Russ.).
Somigliana E, Vigano P, Benaglia L, Busnelli A, Berlanda N, Vercellini P. Management of endometriosis in the infertile patient. Semin Reprod Med. 2017;35:1:31-37. https://doi.org/10.1055/s-0036-1597125
Tanbo T, Fedorcsak P. Endometriosis-associated infertility: aspects of pathophysiological mechanisms and treatment options. Acta Obstet Gynecol Scand. 2017;96:6:659-667. https://doi.org/10.1111/aogs.13082
Latif S, Saridogan E. Endometriosis, oocyte, and embryo quality. J Clin Med. 2023;12:13:4186. https://doi.org/10.3390/jcm12134186
Sanchez AM, Vanni VS, Bartiromo L, Papaleo E, Zilberberg E, Candiani M, Orvieto R, Vigano P. Is the oocyte quality affected by endometriosis? A review of the literature. J Ovarian Res. 2017;10:1:43. https://doi.org/10.1186/s13048-017-0341-4
Dib LA, Araujo MC, Giorgenon RC, Romao GS, Ferriani9 RA, Navarro P. Noninvasive imaging of the meiotic spindle of in vivo matured oocytes from infertile women with endometriosis. Reproductive Sciences. 2013;20:4:456-462. https://doi.org/10.1177/1933719112459217
Tilia L, Venetis C, Kilani S, Cooke S, Chapman M. Is oocyte meiotic spindle morphology associated with embryo ploidy? A prospective cohort study. Fertil Steril. 2016;105:1085-1092.e7. https://doi.org/10.1016/j.fertnstert.2015.12.018
Tilia L, Chapman M, Kilani S, Cooke S, Venetis C. Oocyte meiotic spindle morphology is a predictive marker of blastocyst ploidy — a prospective cohort study. Fertil Steril. 2020;113:1:105-113. https://doi.org/10.1016/j.fertnstert.2019.08.070
Yan J, Wu K, Tang R, Ding L, Chen ZJ. Effect of maternal age on the outcomes of in vitro fertilization and embryo transfer (IVF-ET). Science China Life Sciences. 2012;55:8:694-698. https://doi.org/10.1007/s11427-012-4357-0
Zhu Z, Xu W, Liu L. Ovarian aging: mechanisms and intervention strategies. Med Rev (Berlin). 2022;2:6:590-610. https://doi.org/10.1515/mr-2022-0031
Wasielak-Politowska M, Kordowitzki P. Chromosome segregation in the oocyte: What goes wrong during aging. Int J Mol Sci. 2022;23:5:2880. https://doi.org/10.3390/ijms23052880