Intestinal microbiota, gastroenterological complaints and quality of life in overweight, obese and type 2 diabetes patients

Bakulina N.V.; Tikhonov S.V.; Ermolenko E.I.; Kotyleva M.P.; Lavrenova N.S.; Topalova Yu.G.; Simanenkov V.I.; Suvorov A.N.

doi:https://doi.org/10.17116/profmed20222506180

Bakulina N.V.

I.I. Mechnikov Northwestern State Medical University

Tikhonov S.V.

Research and Clinical Centre of Pediatric Psychoneurology Moscow Department of Public Health;
Medical Institute of the Peoples' Friendship University of Russia

SPIN RSCI: 2185-1960
Scopus AuthorID: 57220106707
ORCID: 0000-0002-5489-3516

Ermolenko E.I.

North-Western State Medical University named after I.I. Mechnikov;
FSBSI «Institute of Experimental Medicine»

Kotyleva M.P.

FSBSI «Institute of Experimental Medicine»

Lavrenova N.S.

FSBSI «Institute of Experimental Medicine»

Topalova Yu.G.

North-Western State Medical University named after I.I. Mechnikov

Simanenkov V.I.

I.I. Mechnikov Northwestern State Medical University

Suvorov A.N.

FSBSI «Institute of Experimental Medicine»

Intestinal microbiota, gastroenterological complaints and quality of life in overweight, obese and type 2 diabetes patients

Authors:

Bakulina N.V., Tikhonov S.V., Ermolenko E.I., Kotyleva M.P., Lavrenova N.S., Topalova Yu.G., Simanenkov V.I., Suvorov A.N.

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Journal: Russian Journal of Preventive Medicine. 2022;25(6): 80‑88

DOI: 10.17116/profmed20222506180

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To cite this article:

Bakulina NV, Tikhonov SV, Ermolenko EI, et al. . Intestinal microbiota, gastroenterological complaints and quality of life in overweight, obese and type 2 diabetes patients. Russian Journal of Preventive Medicine. 2022;25(6):80‑88. (In Russ.)
https://doi.org/10.17116/profmed20222506180

Подписка 2026 Профилактическая медицина (Russian Journal of Preventive Medicine)

Использование инфографики авторами научных работ

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PURPOSE OF THE STUDY

To study the quantitative and qualitative characteristics of the intestinal microbiota, gastroenterological complaints and quality of life in overweight, obese and type 2 diabetes patients.

MATERIAL AND METHODS

The study involved 51 overweight and obese patients. Patients were determined by weight, height, waist circumference; intestinal microbiota was studied by real-time polymerase chain reaction. Plasma concentrations of alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, gamma-glutamine transpeptidase, bilirubin by fractions, albumin, glucose, insulin, lactate dehydrogenase, triglycerides, total cholesterol, low-density lipoproteins, high-density lipoproteins, C-reactive protein were determined in patients. Patients completed the GSRS, SF-36 questionnaires. The study compared the gut microbiota in overweight and obese patients with and without type 2 diabetes. Clinical, physical, laboratory parameters were determined that correlated with the concentration of intestinal bacteria

RESULTS

The study involved 51 patients: 19 (37%) men and 32 (63%). The median age was 54 (44; 63) years, the median body mass index was 30.99 (28.62; 34.44) kg/m². Abdominal obesity was detected in 46 (90.2%) patients. 28 (54.9%) patients had diabetes mellitus. Differences between patients with and without type 2 diabetes: total bacterial mass — 12.00 [10.00; 12.78] vs 10.78 [9.78; 11.48] (p=0.004); Lactobacillus spp. — 0.00 [0.00; 5.00] vs 5.00 [5.00; 5.70] (p<0.001); Escherichia coli — 8.30 [6.90; 9.00] vs 6.60 [6.30; 7.78] (p<0.001); Bacteroides spp. — 11.92 [10.70; 13.00] vs 10.78 [9.78; 11.30] (p<0.001); Bacteroides thetaiotaomicron — 7.30 [0.00; 9.00] vs. 0.00 [0.00; 6.95] (p=0.010); Akkermansia muciniphila — 0.00 [0.00; 0.00] vs 6.30 [0.00; 8.48] log10 CFU/g (p=0.050). Against the background of increasing of the waist circumference, the concentration of Lactobacillus spp. in the stool was decreasing, while the concentrations of E. coli, Bacteroides spp. and S. aureus was increasing. The increase in the concentration of A. muciniphila and Lactobacillus spp. was accompanied by a decrease in biochemical markers of metabolic disorders.

CONCLUSION

The study showed that the concentration of intestinal bacteria is associated with the presence of diabetes mellitus and other metabolic disorders.

Keywords:

overweight

obesity

waist circumference

diabetes mellitus

metabolic syndrome

microbiota

microbiocenosis

A.muciniphila

Lactobacillus spp

Authors:

Bakulina N.V.

I.I. Mechnikov Northwestern State Medical University

Tikhonov S.V.

Research and Clinical Centre of Pediatric Psychoneurology Moscow Department of Public Health;
Medical Institute of the Peoples' Friendship University of Russia

SPIN RSCI: 2185-1960
Scopus AuthorID: 57220106707
ORCID: 0000-0002-5489-3516

Ermolenko E.I.

North-Western State Medical University named after I.I. Mechnikov;
FSBSI «Institute of Experimental Medicine»

Kotyleva M.P.

FSBSI «Institute of Experimental Medicine»

Lavrenova N.S.

FSBSI «Institute of Experimental Medicine»

Topalova Yu.G.

North-Western State Medical University named after I.I. Mechnikov

Simanenkov V.I.

I.I. Mechnikov Northwestern State Medical University

Suvorov A.N.

FSBSI «Institute of Experimental Medicine»

Received:

25.01.2022

Accepted:

28.04.2022

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