The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Gumenyuk L.N.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Dobrokhotova Yu.E.

Federal State Autonomous Educational Institution of Higher Education «N.I. Pirogov Russian National Research Medical University» of the Ministry of Health of the Russian Federation

Puchkina G.A.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Urezko O.A.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Kunitsyna E.Yu.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Stolyarova S.V.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Koblichenko S.V.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Changes in the intestinal microbiota and their relationship with some indicators of the cytokine profile in patients with external genital endometriosis

Authors:

Gumenyuk L.N., Dobrokhotova Yu.E., Puchkina G.A., Urezko O.A., Kunitsyna E.Yu., Stolyarova S.V., Koblichenko S.V.

More about the authors

Journal: Russian Journal of Human Reproduction. 2023;29(6): 87‑94

DOI: 10.17116/repro20232906187

Views: 1063

Downloaded: 12

Download PDF (RU)
Contact the author
Table of contents

CHANGES IN THE INTESTINAL MICROBIOTA AND THEIR RELATIONSHIP WITH SOME INDICATORS OF THE CYTOKINE PROFILE IN PATIENTS WITH EXTERNAL GENITAL ENDOMETRIOSIS
CHANGES IN THE INTESTINAL MICROBIOTA AND THEIR RELATIONSHIP WITH SOME INDICATORS OF THE CYTOKINE PROFILE IN PATIENTS WITH EXTERNAL GENITAL ENDOMETRIOSIS

To cite this article:

Gumenyuk LN, Dobrokhotova YuE, Puchkina GA, Urezko OA, Kunitsyna EYu, Stolyarova SV, Koblichenko SV. Changes in the intestinal microbiota and their relationship with some indicators of the cytokine profile in patients with external genital endometriosis. Russian Journal of Human Reproduction. 2023;29(6):87‑94. (In Russ.)
https://doi.org/10.17116/repro20232906187

Подписка 2025-2 (Russian Journal of Human Reproduction)
 
МСФ на ЯМ
Close metadata
Recommended articles:
Effi­ciency of using dienogest at the stage of pregravid preparation on the course of pregnancy and the outcome of childbirth in patients with endo­metriosis. Russian Journal of Human Reproduction. 2024;(5):118-125
Inte­rleukin-6 as a marker of infe­ction gene­ralization in neonatal sepsis. Russian Journal of Anesthesiology and Reanimatology. 2024;(3):35-42
Expe­rience of combined treatment of patients with infe­rtility operated for exte­rnal geni­tal endo­metriosis. Russian Bulletin of Obstetrician-Gynecologist. 2024;(3):77-81
Mole­cular markers of M. tube­rculosis viru­lence in lung tissue (experimental study). Russian Journal of Archive of Pathology. 2024;(4):31-37
Mono­cytic profile of patients after pelvic floor reco­nstruction. Russian Bulletin of Obstetrician-Gynecologist. 2024;(4):29-34
Impact of alco­hol consumption on nature of nutrition, meta­bolism and human target organs. Part 1. Meta­bolic dysfunction. Russian Journal of Preventive Medi­cine. 2024;(8):87-93
Age-related features of the gut microbiota composition of Rhesus monkeys kept in captivity. Mole­cular Gene­tics, Microbiology and Viro­logy. 2024;(3):22-28
The effect of ethy­lmethylhydroxypyridine succinate on the para­meters of chro­nic neuroinflammation and plastic processes in the brain of old rats during course of dexa­methasone admi­nistration. S.S. Korsakov Journal of Neurology and Psychiatry. 2024;(9):115-121
Modu­lation of oxidative stress as an early symptom of sarcopenia in type 2 diabetes mellitus. Russian Journal of Preventive Medi­cine. 2024;(10):108-116
Impact of alco­hol consumption on nature of nutrition, meta­bolism and human target organs. Part 2. Basic principles of nutritional inte­rvention. Russian Journal of Preventive Medi­cine. 2024;(10):131-136

Purpose of the study. To study changes in the taxonomic composition of the intestinal microbiota and evaluate at the genus level the nature of their relationship with the levels of IL-6, IL-8 and TNF-α in the blood plasma in patients with external genital endometriosis.

MATERIAL AND METHODS

The composition of the intestinal microbiota in 55 patients with external genital endometriosis (EGE) and 50 healthy women was assessed by 16S ribosomal ribonucleic acid sequencing. The levels of IL-6, IL-8 and TNF-α in blood plasma were studied using enzyme-linked immunosorbent assay. A correlation analysis of the relationship between the number of intestinal bacteria and the levels of IL-6, IL-8 and TNF-α was carried out.

RESULTS

In patients with NGE, changes in the taxonomic composition of the intestinal microbiota were found: a statistically significant decrease in the number of Eubacterium, Coprococcus, Ruminococcus, Bacterioides, Turicibacter, Prevotella and an increase in the number of Shigella. A direct correlation between the abundance of Shigella and the IL-8 index was established, and a reverse correlation between the abundance of Prevotella and Ruminococcus and the TNF-α index.

CONCLUSION

Additional data were obtained on the peculiarities of changes in the intestinal microbiota of patients with external genital endometriosis and the connection of certain genera with plasma levels of IL-8 and TNF-α in this pathology, which may become the basis for further research, as well as for the formation of new approaches to the treatment of external genital endometriosis. endometriosis.

Keywords:

external genital endometriosis
intestinal microbiota
IL-6
IL-8
TNF-α

Authors:

Gumenyuk L.N.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Dobrokhotova Yu.E.

Federal State Autonomous Educational Institution of Higher Education «N.I. Pirogov Russian National Research Medical University» of the Ministry of Health of the Russian Federation

Puchkina G.A.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Urezko O.A.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Kunitsyna E.Yu.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Stolyarova S.V.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Koblichenko S.V.

V.I. Vernadsky Crimean Federal University Ministry of Science and Higher Education of the Russian Federation

Received:

08.12.2022

Accepted:

09.01.2023

List of references:

  1. Zondervan KT, Becker CM, Koga K, et al. Endometriosis. Nature Reviews. Disease Primers. 2018;4:9.  https://doi.org/10.1038/s41572-018-0008-5
  2. Saunders PTK, Horne AW. Endometriosis: Etiology, pathobiology, and therapeutic prospects. Cell. 2021;184(11):2807-2824. https://doi.org/10.1016/j.cell.2021.04.041
  3. Bao C, Wang H, Fang H. Genomic Evidence Supports the Recognition of Endometriosis as an Inflammatory Systemic Disease and Reveals Disease-Specific Therapeutic Potentials of Targeting Neutrophil Degranulation. Frontiers in Immunology. 2022;23(13):758440. https://doi.org/10.3389/fimmu.2022.758440
  4. Greene R, Stratton P, Cleary SD, et al. Diagnostic experience among 4,334 women reporting surgically diagnosed endometriosis. Fertility and Sterility. 2009;91(1):32-39. 
  5. Greenbaum H, Bat-El L, Galper BEL, et al. Endometriosis and autoimmunity: Can autoantibodies be used as a non-invasive early diagnostic tool? Autoimmunity Reviews. 2021;20(5):102795.
  6. Clement PB. The Pathology of Endometriosis: A Survey of the Many Faces of a Common Disease Emphasizing Diagnostic Pitfalls and Unusual and Newly Appreciated Aspects. Advances in Anatomic Pathology. 2007;14(4):241-260.  https://doi.org/10.1097/PAP.0b013e3180ca7d7b
  7. Taylor HS, Kotlyar AM, Flores VA. Endometriosis is a chronic systemic disease: clinical challenges and novel innovations. Lancet. 2021;27:397(10276):839-852.  https://doi.org/10.1016/S0140-6736(21)00389-5
  8. Yarmolinskaya MI. Cytokine profile of peritoneal fluid and peripheral blood of patients with external genital endometriosis. Zhurnal akusherstva i zhenskih bolezneǐ. 2008;57(3):30-34. (In Russ.).
  9. Sikora J, Smycz-Kubańska M, Mielczarek-Palacz A, Kondera-Anasz Z. Abnormal peritoneal regulation of chemokine activation — the role of IL-8 in pathogenesis of endometriosis. American Journal of Reproductive Immunology. 2017;77(4):12622. https://doi.org/10.1111/aji.12622
  10. Cameron MJ, Kelvin DJ. Cytokines and chemokines — their receptors and their genes: an overview. Advances in Experimental Medicine and Biology. 2003;520:8-32.  https://doi.org/10.1007/978-1-4615-0171-82
  11. Somigliana E, Viganò P, Tirelli AS, et al. Use of the concomitant serum dosage of CA 125, CA 19-9 and interleukin-6 to detect the presence of endometriosis. Results from a series of reproductive age women undergoing laparoscopic surgery for benign gynaecological conditions. Human Reproduction. 2004;19(8):1871-1876. https://doi.org/10.1093/humrep/deh312
  12. Dong Hao Lu, Song H, Shi G. Anti-TNF-α treatment for pelvic pain associated with endometriosis. Cochrane Database of Systematic Reviews. 2010;3(3):CD008088. https://doi.org/10.1002/14651858
  13. Li A, Dubey S, Varney ML, et al. IL-8 directly en- hanced endothelial cell survival, proliferation, and matrix metalloproteinases production and regulated angiogenesis. Journal of Immunology. 2003; 170(6):3369-3376.
  14. Malvezzi H, Hernandes C, Piccinato CA, Podgaec S. Interleukin in endometriosis-associated infertility-pelvic pain: systematic review and meta-analysis. Reproduction. 2019;158(1):1-12.  https://doi.org/10.1530/REP-18-0618
  15. Scholl B, Bersinger NA, Kuhn A. Correlation between symptoms of pain and peritoneal fluid inflammatory cytokine concentrations in endometriosis. Gynecological Endocrinology. 2009;25(11):701-706.  https://doi.org/10.3109/09513590903159680
  16. Vinolo MAR, Rodrigues HG, Nachbar RT, Curi R. Regulation of Inflammation by Short Chain Fatty Acids. Nutrients. 2011;3(10): 858-876.  https://doi.org/10.3390/nu3100858
  17. Kamo T, Akazawa H, Suzuki JI, Komuro I. Novel Concept of a Heart-Gut Axis in the Pathophysiology of Heart Failure. Korean Circulation Journal. 2017;47(5):663-669.  https://doi.org/10.4070/kcj.2017.0028
  18. Svensson A, Brunkwall L, Roth B, et al. Associations Between Endometriosis and Gut Microbiota. Reproductive Sciences. 2021;28(8): 2367-2377. https://doi.org/10.1007/s43032-021-00506-5
  19. Chen S, Gu Z, Zhang W, et al. The study of endometriosis and adenomyosis related microbiota in female lower genital tract in Northern Chinese population. Gynecology and Obstetrics Clinical Medicine. 2021;1(3):119-129.  https://doi.org/10.1016/j.gocm.2021.07.007
  20. Vallejo V, Ilagan JG. A Postpartum Death Due to Coronavirus Disease 2019 (COVID-19) in the United States. Obstetrics and Gynecology. 2020;136(1):52-55. 
  21. Caslin B, Maguire C, Karmakar A, et al. Alcohol shifts gut microbial networks and ameliorates a murine model of neuroinflammation in a sex-specific pattern. Proceedings of the National Academy of Sciences of the United States of America. 2019;116(51):25808-25815. https://doi.org/10.1073/pnas.1912359116
  22. Bernstein CN, Forbes JD. Gut Microbiome in Inflammatory Bowel Disease and Other C hronic Immune-Mediated Inflammatory Diseases. Inflammatory Intestinal Diseases. 2017;2(2):116-123.  https://doi.org/10.1159/000481401
  23. Larsen JM. The immune response to Prevotella bacteria in chronic inflammatory disease. Immunology. 2017;151(4):363-374.  https://doi.org/10.1111/imm.12760
  24. Ata B, Yildiz S, Turkgeldi E, et al. The Endobiota Study: Comparison of Vaginal, Cervical and Gut Microbiota Between Women with Stage 3/4 Endometriosis and Healthy Controls. Scientific Reports. 2019;9(1):2204. https://doi.org/10.1038/s41598-019-39700-6
  25. Gumenyuk LN, Golod MV, Silaeva NV, et al. Changes in the intestinal microbiota and their relationship with the severity of the disease and some indicators of the cytokine profile in patients with COVID-19. Vestnik RGMU. 2022;(1):23-30. (In Russ.). https://doi.org/10.24075/vrgmu.2022.006
  26. Tagini F, Greub G. Bacterial genome sequencing in clinical microbiology: a pathogen-oriented review. European Journal of Clinical Microbiology and Infectious Diseases. 2017;36(11):2007-2020. https://doi.org/10.1007/s10096-017-3024-6
  27. Farmer JJ, Farmer MK, Holmes B. The Enterobacteriaceae: General Characteristics. Topley & Wilson’s Microbiology and Microbial Infections. 2010;2:1317-1359. https://doi.org/10.1002/9780470688618.taw0051
  28. Kodati VL. Role of Shigella infection in endometriosis: a novel hypothesis. Medical Hypotheses. 2008;70(2):239-243. 
  29. Chadchan SB, Cheng M, Parnell LA, et al. Antibiotic therapy with metronidazole reduces endometriosis disease progression in mice: a potential role for gut microbiota. Human Reproduction. 2019;34(6): 1106-1116. https://doi.org/10.1093/humrep/dez041
  30. Raqib R, Wretlind B, Andersson J, Lindberg AA. Cytokine secretion in acute shigellosis is correlated to disease activity and directed more to stool than to plasma. Journal of Infectious Diseases. 1995; 171:376-384.  https://doi.org/10.1093/infdis/171.2.376
  31. Larsen JM. The immune response to prevotella bacteria in chronic inflammatory disease. Immunology. 2017;151:363-374.  https://doi.org/10.1111/imm.12760
  32. Khan KN, Fujishita A, Hiraki K, et al. Bacterial contamination hypothesis: a new concept in endometriosis. Reproductive Medicine and Biology. 2018;17(2):125-133.  https://doi.org/10.1002/rmb2.12083
  33. Gargari G. Consumption of a bifidobacterium bifidum strain for 4 weeks modulates dominant intestinal bacterial taxa and fecal butyrate in healthy adults. Applied and Environmental Microbiology. 2016; 82:5850. https://doi.org/10.1128/AEM.01753-16
  34. Gonçalves P, Araújo J, Di Santo J. A cross-talk between microbiota-derived short-chain fatty acids and the host mucosal immune system regulates intestinal homeostasis and inflammatory bowel disease. Inflammatory Bowel Diseases. 2018;24:558-572.  https://doi.org/10.1093/ibd/izx029
  35. Kelly CJ, Zheng L, Campbell EL, et al. Crosstalk between Microbiota-Derived Short-Chain Fatty Acids and Intestinal Epithelial HIF Augments Tissue Barrier Function. Cell Host Microbe. 2015; 17(5):662-671.  https://doi.org/10.1016/j.chom.2015.03.005
  36. Lakshmanan AP, Al Zaidan S, Bangarusamy DK, et al. Increased Relative Abundance of Ruminoccocus Is Associated With Reduced Cardiovascular Risk in an Obese Population. Frontiers in Nutrition. 2022;9:849005. https://doi.org/10.3389/fnut.2022.849005
  37. Chadchan SB, Popli P, Ambati CR, et al. Gut microbiota-derived short-chain fatty acids protect against the progression of endometriosis. Life Science Alliance. 2021;4(12):e202101224. https://doi.org/10.26508/lsa.202101224
  38. Dobreva ZG, Grigorov BG, Stanilova SA. Effect of a Histone Deacetylases Inhibitor of IL-18 and TNF-Alpha Secretion in Vitro. Open Access Macedonian Journal of Medical Sciences. 2018;10:6(2): 269-273.  https://doi.org/10.3889/oamjms.2018.075
  39. Samartzis EP, Noske A, Samartzis N, et al. The expression of histone deacetylase 1, but not other class I histone deacetylases, is significantly increased in endometriosis. Reproductive Sciences. 2013; 20(12):1416-1422. https://doi.org/10.1177/1933719113488450

Close metadata

Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.