Purpose of the study. To study the effect of different methods of embryo culture on the frequency of high-quality blastocysts obtained, the frequency of incomplete cycles in patients of late reproductive age, patients with a poor response to gonadotropins and patients with repeated implantation failures.
MATERIAL AND METHODS
The results of embryo cultivation using: a) traditional technology (TT) in 1563 cycles were assessed; b) TLT incubator in 386 cycles. The frequency of obtained blastocysts and the frequency of incomplete cycles were assessed. The results were compared in selectively selected groups of women: 1) with a good prognosis for the success of in vitro fertilization; 2) over 36 years old; 3) with a poor response to gonadotropins; 4) with repeated implantation failures.
RESULTS
In patients over 36 years of age, with a poor response to gonadotropins and repeated implantation failures, compared with patients without these characteristics (control group), there was a decrease in the frequency of blastocysts obtained from zygotes by 8-15% and an increase in the frequency of incomplete cycles by 12-15%. In all selected groups with the analyzed factors, when using a TLT incubator instead of TT, deviations of the studied parameters of embryogenesis from their values in the control group were less pronounced. In women over 36 years of age, the use of a TLT incubator instead of a TT is both
produced a significant increase in the resulting blastocysts from 12.8 to 19.3% (p=0.034) and reduced the frequency of incomplete cycles from 24.4 to 17.7% (p=0.031).
CONCLUSIONS
Time-lapse embryo culture technology, instead of the traditional technique, significantly improves embryogenesis rates (increasing the frequency of resulting blastocysts and reducing the frequency of incomplete cycles) in a cohort of patients over 36 years of age and has a less pronounced effect in cohorts of women with a poor response and repeated implantation failures.
Key words: infertility, IVF, time-lapse technology of embryo cultivation.
