Features of mRNA expression of transcription factors during physiological full-term pregnancy

Kaganova M.A.; Spiridonova N.V.

doi:https://doi.org/10.17116/repro2022280516

Kaganova M.A.

Samara State Medical University

Spiridonova N.V.

Samara State Medical University

Features of mRNA expression of transcription factors during physiological full-term pregnancy

Authors:

Kaganova M.A., Spiridonova N.V.

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Journal: Russian Journal of Human Reproduction. 2022;28(5): 6‑13

DOI: 10.17116/repro2022280516

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Kaganova MA, Spiridonova NV. Features of mRNA expression of transcription factors during physiological full-term pregnancy. Russian Journal of Human Reproduction. 2022;28(5):6‑13. (In Russ.)
https://doi.org/10.17116/repro2022280516

Подписка 2026 Проблемы репродукции (Russian Journal of Human Reproduction)

Использование инфографики авторами научных работ

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INTRODUCTION

Pregnancy is a special condition that challenges the maternal immune system, and the study of the ratio of immune cells locally in the fetoplacental complex at different stages of physiological pregnancy is an important step in understanding the foundations of the pathogenesis of various obstetric complication.

OBJECTIVE

To assess the level of mRNA expression of transcriptional factors (TF) of T-helpers (GATA3, RORC2, FOXP3) in the cervical canal, placenta and fetal membranes during full-term pregnancy.

MATERIALS AND METHODS

Pregnant women (n=42) were included in this reserch with a physiological pregnancy at gestation period 37—41 weeks. They underwent a planned caesarean section at the Samara state Hospital N1 n.a. N.I. Pirogov. Indications were abnormal position and presentation of the fetus and/or a scar after previous caesarean section. The mRNA expression GATA3, RORC2, FOXP3 in the cervical canal, placenta and fetal membranes was determined by RT-PCR at the laboratory of molecular genetic methods LLC «DNA-technology» using reagents «Proba NK».

RESULTS

MRNA expression GATA3 in the cervical canal, placenta and fetal membranes was observed in 100% and was hundreds of times higher than the mRNA expression RORC2, FOXP3. The frequency of mRNA expression RORC2 in the cervical canal was 69%, fetal membranes — 45%, placenta — 76%; for FOXP3: 40.5%, 54.7% and 88.1%, respectively. mRNA Expression RORC2, FOXP3 had significant positive correlations with each other and did not correlate with the level of GATA3.

CONCLUSIONS

Understanding the features of mRNA expression of TF at various loci of the fetoplacental complex during physiological pregnancy contributes to the awareness of immunological interactions on the eve of childbirth and can serve as an initial stage in the study of changes in the immune system in obstetric pathology.

Keywords:

mRNA

transcription factors

GATA3

RORC2

FOXP3

polymerase chain reaction

pregnancy

placenta

Authors:

Kaganova M.A.

Samara State Medical University

Spiridonova N.V.

Samara State Medical University

Received:

02.12.2021

Accepted:

10.06.2022

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References:

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