OBJECTIVE

To determine the significance of polymorphic variants of the genes of the renin-angiotensin system (rs5186 of the AGTR1 gene; rs11091046 of the AGTR2 gene), the prooxidant-antioxidant system (s1049255 (A640G) of the CYBA gene; rs2333227 (G463A) of the MPO gene ; vs4880 (V16 growth factor) of endothelial vascular endothelial vascular disease (rs3025039 (C936T) and rs699947 of the VEGF-A gene) as genetic predictors of preeclampsia development.

MATERIAL AND METHOD

The study involved 197 pregnant women aged 17—40 years, representing 2 groups: the main (97 pregnant women suffering from moderate to severe PE) and control (100 patients with a physiological pregnancy). Venous blood of pregnant women was used as biological samples. DNA was isolated by the standard phenol-chloroform extraction protocol. The allelic state of single-nucleotide polymorphic markers of genes of the renin-angiotensin system, prooxidant-antioxidant system, and vascular endothelial growth factor was determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis using specific primers and corresponding restriction enzymes produced by SibE NPO produced by SibE.

RESULTS

Reliable genotypic markers of a hereditary predisposition to preeclampsia were determined: homozygous carriage of the C allele rs11091046 in the angiotensin II receptor gene (AGTR2), homozygous carriage of the C allele rs4880 (A16V) in the mitochondrial homozygous igelodigodyloid superoxide «Rs3025039 (C936T) in the gene for vascular endothelial growth factor (VEGF-A).

CONCLUSIONS

Genotypic predictors for the development of PE are: homozygous carriage of the C allele rs11091046 in the angiotensin II receptor gene (AGTR2), homozygous carriage of the C allele rs4880 (A16V) in the mitochondrial superoxide dismutase (SOD2) r50 gene and 30 homozygous homozygous allele C936T) in the vascular endothelial growth factor (VEGF-A) gene.