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Nemchenko U.M.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Belkova N.L.
Scientific Centre «Family Health and Human Reproduction Problems»
Pogodina A.V.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Romanitsa A.I.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Novikova E.A.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Klimenko E.S.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Grigorova E.V.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Kungurtseva E.A.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Butakova K.O.
ISU
Rychkova L.V.
Scientific Centre for Family Health and Human Reproduction Problems
Features of the composition of bifidoflora in the intestinal microbiome of obese adolescents
Journal: Molecular Genetics, Microbiology and Virology. 2021;39(3): 31‑38
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To cite this article:
Nemchenko UM, Nemchenko UM, Belkova NL, et al. . Features of the composition of bifidoflora in the intestinal microbiome of obese adolescents. Molecular Genetics, Microbiology and Virology.
2021;39(3):31‑38. (In Russ.)
https://doi.org/10.17116/molgen20213903131
Was to compare the composition of the bifidoflora of the intestinal microbiota of obese adolescents and adolescents from a healthy control group using molecular genetic methods to identify potential microbial characteristics of obesity.
Samples of biological material were obtained from the clinic of the Scientific Centre for Family Health and Human Reproduction Problems. Adolescents aged 11 to 16 were divided into two groups: obese adolescents and a control group. Metasequencing of the V3—V4 fragment of the 16S rRNA gene and PCR detection of bifidoflora with species-specific primers (Bifidobacterium longum, B. bifidum, B. infantis, B. adolescentis, B. catenulatum) were performed.
Metasequoiale showed that on average the proportion of bifidobacteria in total accounted for 1.2% of the intestinal microbiome. 9 phylotypes of bifidobacteria were identified, of which the most represented were d7ab (B. adolescentis), 43e3 (B. catenulatum) and 1bc4 (B. longum). It was shown that a decrease in the representation of bifidobacteria phylotypes in the structure of the microbiota was associated only with obesity, especially for the species B. longum (p<0.05). PCR also confirmed this dependence for the B. longum, revealed a general decrease in the frequency of all species of bifidobacteria in the group of obese adolescents.
The decrease in the total share of bifidobacteria structure and the low representation of B. longum in the gut microbiota of adolescents is directly related with the disease and might be microbial markers of dysbiotic disorders in obesity.
Keywords:
Authors:
Nemchenko U.M.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Belkova N.L.
Scientific Centre «Family Health and Human Reproduction Problems»
Pogodina A.V.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Romanitsa A.I.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Novikova E.A.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Klimenko E.S.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Grigorova E.V.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Kungurtseva E.A.
Federal State Public Scientific Institution «Scientific Centre for Family Health and Human Reproduction Problems»
Butakova K.O.
ISU
Rychkova L.V.
Scientific Centre for Family Health and Human Reproduction Problems
Received:
12.02.2020
Accepted:
29.05.2020
List of references:
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