Objective: to determine the effectiveness of sequential therapy of atopic dermatitis in children using 0.1% methylprednisolone aceponate in combination with ceramides and topical calcineurin inhibitor, tacrolimus ointment. Material and Methods. A total of 35 patients aged 2–16 years were examined. To assess the effectiveness of the therapy, the dermatological status was described in detail using the SCORAD index (1994), Dermatology Specific Quality of Life (DSQL) was determined before and after the therapy. Results. Therapy using 0.1% methylprednisolone aceponate in combination with ceramides and tacrolimus ointment showed excellent clinical efficacy in 32 (91.4%) patients, good, in 3 patients (8.6%), with no cases of satisfactory and unsatisfactory outcomes. The first clinical effect was noted already on the Day 2 after the start of the treatment. The therapy with 0.1% methylprednisolone aceponate cream in combination with ceramides and tacrolimus ointment was excellently tolerated by 34 (97.1%) patients and well tolerated by 1 patient (2.9%). No serious adverse events were reported during the study. Conclusions. The use of sequential therapy with 0.1% methylprednisolone aceponate cream in combination with ceramides and tacrolimus ointment allows for highly effective and safe local treatment in patients with atopic dermatitis.