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Kvan O.K.

Burdenko Neurosurgical Center

Teryaeva N.B.

Burdenko Neurosurgical Center

Sukhorukova M.V.

Burdenko Neurosurgical Center

Kozlov A.V.

Burdenko Neurosurgical Center

Lubnin A.Yu.

Burdenko Neurosurgical Center

Decolonization of autologous blood with cefuroxime during hardware reinfusion in neurosurgical patients

Authors:

Kvan O.K., Teryaeva N.B., Sukhorukova M.V., Kozlov A.V., Lubnin A.Yu.

More about the authors

Journal: Burdenko's Journal of Neurosurgery. 2024;88(6): 47‑53

DOI: 10.17116/neiro20248806147

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Table of contents

DECOLONIZATION OF AUTOLOGOUS BLOOD WITH CEFUROXIME DURING HARDWARE REINFUSION IN NEUROSURGICAL PATIENTS
DECOLONIZATION OF AUTOLOGOUS BLOOD WITH CEFUROXIME DURING HARDWARE REINFUSION IN NEUROSURGICAL PATIENTS

To cite this article:

Kvan OK, Teryaeva NB, Sukhorukova MV, Kozlov AV, Lubnin AYu. Decolonization of autologous blood with cefuroxime during hardware reinfusion in neurosurgical patients. Burdenko's Journal of Neurosurgery. 2024;88(6):47‑53. (In Russ., In Engl.)
https://doi.org/10.17116/neiro20248806147

Подписка 2025 Журнал «Вопросы нейрохирургии» имени Н.Н. Бурденко (Burdenko's Journal of Neurosurgery)
 
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

Autologous blood obtained during hardware reinfusion can be bacterially contaminated with skin microflora in most cases (up to 90%) regardless of the area of surgical intervention. Increased volume of washing solution for red blood cell management, white blood cell filtration and X-ray irradiation do not always provide complete decontamination.

OBJECTIVE

To analyze the effectiveness of adding cefuroxime to extracorporeal circuit during hardware blood reinfusion.

MATERIAL AND METHODS

The study included 50 patients with intracranial tumors. Hardware blood reinfusion was used due to baseline disease, blood supply to the neoplasm and high risk of intraoperative blood loss. All patients received perioperative antibiotic prophylaxis. We assessed the effectiveness of decontamination using microbiological control at all stages of the procedure. We used the XTRA Liva Nova complex autologous blood recovery system (Popt protocol), PALL Purecell RC2VAE leukocyte filters, BacT/ALERT 3D automatic microbiological analyzer and vials with FA Plus and FN Plus medium and adsorbent. X-ray irradiation of autologous erythrocytes was performed at a dose of 25 Gy (ARDOK-1 device).

RESULTS

Addition of cefuroxime 1.5 g to blood collection reservoir excluded bacterial growth in autologous blood samples from this reservoir in 100% of cases. At subsequent stages of blood processing, we isolated S. epidermidis, S. aureus, S. hominis, S. capitis, as well as Acinetobacter ursingii, Morganella morganii and Klebsiella aerogenes from some samples. Maximum bacterial contamination was observed at the stage of erythrocyte washing (34% of samples). After leukocyte filtration and blood irradiation, this value was 8%.

CONCLUSION

Combination of all decontamination methods (erythrocyte washing in large volume of solution, leukocyte filtration and irradiation) together with adding cefuroxime into extracorporeal circuit excluded bacterial growth in 92% of samples of autologous erythrocytes.

Keywords:

autologous blood
hardware blood reinfusion
bacterial contamination
cefuroxime
decontamination
leukocyte filtration
blood irradiation

Authors:

Kvan O.K.

Burdenko Neurosurgical Center

Teryaeva N.B.

Burdenko Neurosurgical Center

Sukhorukova M.V.

Burdenko Neurosurgical Center

Kozlov A.V.

Burdenko Neurosurgical Center

Lubnin A.Yu.

Burdenko Neurosurgical Center

Received:

22.08.2024

Accepted:

20.09.2024

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