The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 101-59-87
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2024
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Kogan E.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Shchelokova E.E.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Demura T.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Zharkov N.V.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Kichigina O.N.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Kovyazina N.V.

Russian University of Medicine

Mordovina A.I.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Zelenchenkova P.I.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Meerovich G.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University);
A.M. Prokhorov General Physics Institute Russian Academy of Sciences (GPI RAS)

Reshetov I.V.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

ALDH1, CD133, CD34-positive cancer stem cells in lung adenocarcinoma in patients who had a new coronavirus infection and retained the persistence of viral proteins in the lung tissue

Authors:

Kogan E.A., Shchelokova E.E., Demura T.A., Zharkov N.V., Kichigina O.N., Kovyazina N.V., Mordovina A.I., Zelenchenkova P.I., Meerovich G.A., Reshetov I.V.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2024;86(5): 5‑14

DOI: 10.17116/patol2024860515

Views: 565

Downloaded: 48

Download PDF (RU)
Contact the author
Table of contents

To cite this article:

Kogan EA, Shchelokova EE, Demura TA, et al. . ALDH1, CD133, CD34-positive cancer stem cells in lung adenocarcinoma in patients who had a new coronavirus infection and retained the persistence of viral proteins in the lung tissue. Russian Journal of Archive of Pathology. 2024;86(5):5‑14. (In Russ.)
https://doi.org/10.17116/patol2024860515

Подписка 2025 (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Close metadata
Recommended articles:
Alopecia and COVID-19: possible etiopathogenetic variants and therapeutic approach. Problems of Balneology, Physiotherapy and Exercise Therapy. 2023;(5):56-63
Medi­cal reha­bilitation features of patients suffered from new coro­navirus infe­ction. Problems of Balneology, Physiotherapy and Exercise Therapy. 2023;(5):64-69
New coro­navirus infe­ction in the II trimester of pregnancy: peri­natal and mate­rnal consequences. Russian Bulletin of Obstetrician-Gynecologist. 2023;(6):34-41
Type 2 diabetes mellitus and COVID-19: lethality predictors. Russian Journal of Preventive Medi­cine. 2023;(11):53-56
Symptoms in the SARS-CoV-2 survivors with critical lung damage (CT-4). Russian Journal of Preventive Medi­cine. 2023;(11):69-77
Left ventricular thro­mbosis and acute heart failure in patients after SARS-CoV-2 infe­ction. Russian Journal of Preventive Medi­cine. 2023;(11):78-81
Clinical, instrumental and labo­ratory data on consequences of COVID-19 in delayed period after admi­ssion. Russian Cardiology Bulletin. 2023;(4):56-66
Asse­ssment of labo­ratory para­meters at various COVID-19 phases in patients with gastrointestinal symptoms. Russian Journal of Evidence-Based Gastroenterology. 2023;(4):89-94
Prospects for predicting preterm labor. Russian Bulletin of Obstetrician-Gynecologist. 2023;(6-2):127-134
Complications and gestational outcomes in women with new SARS-CoV-2 coro­navirus infe­ction during pregnancy. Russian Bulletin of Obstetrician-Gynecologist. 2023;(6-2):167-172

Lung cancer occupies a leading position in the structure of global cancer morbidity and mortality, due to the biological properties of the key pool of tumor cells — cancer stem cells (CSCs). The effects of SARS-CoV2 on tumor CSCs and its niche have not been studied.

OBJECTIVE

To study CSCs in lung adenocarcinomas after COVID19.

MATERIAL AND METHODS

Surgical material from lung adenocarcinoma from 12 patients who had a new coronavirus infection and from 12 patients who did not have COVID19 was examined. Analysis of clinical and anamnestic data, macroscopic and microscopic examination of tumor samples and adjacent intact tissue, immunohistochemical reactions using antibodies to virus proteins and CSC markers ALDH1, CD133 and CD34 were performed.

RESULTS

Adenocarcinoma samples from patients in the main group showed a significant increase in the number of cells expressing the CSCs markers ALDH1, CD133 and CD34 compared to adenocarcinoma samples from control group patients without SARS-CoV2 infection. We found an increase in the number of CSCs in patients with adenocarcinoma metastasis in lymph nodes in both the main and control groups. CSCs of lung adenocarcinomas from SARS-CoV2 survivors contain virus proteins Nucleocapside and Spike protein.

CONCLUSIONS

We found an increase in the number of CSCs with expression of ALDH1, CD133 and CD34 in lung adenocarcinoma in patients with new coronavirus infection. Increased number of ALDH1+, CD133+ CD34+ CSCs in tumor tissue enhance the metastatic potential of lung adenocarcinoma. The Nucleocapsid and Spike proteins of SARS-CoV2 virus are detectable in lung tissue from patients with new coronavirus infection, both in adenocarcinoma cells, CSCs, and in type II pneumocytes, macrophages, and endothelial cells, suggesting prolonged persistence of the virus proteins and probably the virus.

Keywords:

cancer stem cells
lung adenocarcinoma
carcinogenesis
COVID19
ALDH1
CD133
CD34
persistence of Nucleocapside and Spike proteins of SARS-CoV2 in lung tissue
persistence of SARS-CoV2 virus in lung tissue
cancer progression
PDT

Authors:

Kogan E.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Shchelokova E.E.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Demura T.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Zharkov N.V.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Kichigina O.N.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Kovyazina N.V.

Russian University of Medicine

Mordovina A.I.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Zelenchenkova P.I.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Meerovich G.A.

I.M. Sechenov First Moscow State Medical University (Sechenov University);
A.M. Prokhorov General Physics Institute Russian Academy of Sciences (GPI RAS)

Reshetov I.V.

I.M. Sechenov First Moscow State Medical University (Sechenov University)

Received:

12.06.2024

Accepted:

26.06.2024

List of references:

  1. Bray F, Laversanne M, Sung H, Ferlay J, Siegel RL, Soerjomataram I, Jemal A. Global cancer statistics 2022: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2024;74(3):229‐263.  https://doi.org/10.3322/caac.21834
  2. Prabavathy D, Swarnalatha Y, Ramadoss N. Lung cancer stem cells-origin, characteristics and therapy. Stem Cell Investig. 2018;5:6.  https://doi.org/10.21037/sci.2018.02.01
  3. Hardavella G, George R, Sethi T. Lung cancer stem cells-characteristics, phenotype. Transl Lung Cancer Res. 2016;5(3):272-279.  https://doi.org/10.21037/tlcr.2016.02.01
  4. Kogan EA, Meerovich GA, Karshieva SS, Makarova EA, Romanishkin ID, Akhlyustina EV, Meerovich IG, Zharkov NV, Koudan EV, Demura TA, et al. Photodynamic therapy of lung cancer with photosensitizers based on polycationic derivatives of synthetic bacteriochlorin (experimental study). Photodiagnosis Photodyn Ther. 2023;42:103647. https://doi.org/10.1016/j.pdpdt.2023.103647
  5. Park SY, Nam JS. The force awakens: metastatic dormant cancer cells. Exp Mol Med. 2020;52(4):569-581.  https://doi.org/10.1038/s12276-020-0423-z
  6. Behl T, Kaur I, Aleya L, Sehgal A, Singh S, Sharma N, Bhatia S, Al-Harrasi A, Bungau S. CD147-spike protein interaction in COVID-19: get the ball rolling with a novel receptor and therapeutic target. Sci Total Environ. 2022;808:152072. https://doi.org/10.1016/j.scitotenv.2021.152072
  7. Zhang X, Tian T, Zhang X, Liu C, Fang X. Elevated CD147 expression is associated with shorter overall survival in non-small cell lung cancer. Oncotarget. 2017;8(23):37673-37680. https://doi.org/10.18632/oncotarget.16948
  8. Tang Y, Nakada MT, Kesavan P, McCabe F, Millar H, Rafferty P, Bugelski P, Yan L. Extracellular matrix metalloproteinase inducer stimulates tumor angiogenesis by elevating vascular endothelial cell growth factor and matrix metalloproteinases. Cancer Res. 2005;65(8):3193-3199. https://doi.org/10.1158/0008-5472.CAN-04-3605
  9. Muramatsu T. Basigin (CD147), a multifunctional transmembrane glycoprotein with various binding partners. Biochem J. 2016;159(5): 481-490.  https://doi.org/10.1093/jb/mvv127
  10. Kang MJ, Kim HP, Lee KS, Yoo YD, Kwon YT, Kim KM, Kim TY, Yi EC. Proteomic analysis reveals that CD147/EMMPRIN confers chemoresistance in cancer stem cell-like cells. Proteomics. 2013;13(10-11):1714-1725. https://doi.org/10.1002/pmic.201200511
  11. Huang Y, Zhou H, Wang Y, Xiao L, Qin W, Li L. A comprehensive investigation on the receptor BSG expression reveals the potential risk of healthy individuals and cancer patients to 2019-nCoV infection. Aging (Albany NY). 2024;16(6):5412-5434. https://doi.org/10.18632/aging.205655
  12. Gosain R, Abdou Y, Singh A, Rana N, Puzanov I, Ernstoff MS. COVID-19 and cancer: a comprehensive review. Curr Oncol Rep. 2020;22(5):53.  https://doi.org/10.1007/s11912-020-00934-7
  13. Aramini B, Masciale V, Samarelli AV, Tonelli R, Cerri S, Clini E, Stella F, Dominici M. Biological effects of COVID-19 on lung cancer: can we drive our decisions. Front Oncol. 2022;12:1029830. https://doi.org/10.3389/fonc.2022.1029830
  14. Finn OJ. Immuno-oncology: understanding the function and dysfunction of the immune system in cancer. Ann Oncol. 2012;23 Suppl. 8(Suppl. 8):viii6-9.  https://doi.org/10.1093/annonc/mds256
  15. Zarubin EA, Kogan EA. Pathogenesis and morphological changes in the lung in COVID-19. Russian Journal of Archive of Pathology. 2021;83(6):54-59. (In Russ.). https://doi.org/10.17116/patol20218306154
  16. Lei HM, Zhang KR, Wang CH, Wang Y, Zhuang GL, Lu LM, Zhang J, Shen Y, Chen HZ, Zhu L. Aldehyde dehydrogenase 1a1 confers erlotinib resistance via facilitating the reactive oxygen species-reactive carbonyl species metabolic pathway in lung adenocarcinomas. Theranostics. 2019;9(24):7122-7139. https://doi.org/10.7150/thno.35729
  17. Barzegar Behrooz B, Syahir A, Ahmad S. CD133: beyond a cancer stem cell biomarker. J Drug Target. 2019;27(3):257-269.  https://doi.org/10.1080/1061186X.2018.1479756
  18. Radu P, Zurzu M, Paic V, Bratucu M, Garofil D, Tigora A, Georgescu V, Prunoiu V, Pasnicu C, Popa F, et al. CD34-structure, functions and relationship with cancer stem cells. Medicina (Kaunas). 2023;59(5):938.  https://doi.org/10.3390/medicina59050938
  19. Kapoor S, Shenoy S, Bose B. CD34 cells in somatic, regenerative and cancer stem cells: developmental biology, cell therapy, and omics big data perspective. J Cell Biochem. 2020;121(5-6):3058-3069. https://doi.org/10.1002/jcb.29571
  20. WHO Classification of Tumours. Thoracic tumours. WHO classification of tumours editorial board. 5th ed. Vol. 5. France, Lyon: IARC; 2021. Available at: https://tumourclassification.iarc.who.int
  21. Korzhevskii DE, Gilyarov AV. Osnovy gistologicheskoi tekhniki. Prakticheskoe rukovodstvo. Moscow: Meditsina; 2010. (In Russ.).
  22. Dabbs DJ. Diagnostic immunohistochemistry. New York: Churchill Livingstone; 2002. 673p.
  23. Blagova OV, Kogan EA, Lutokhina YuA, Savina PO, Ainetdinova DKh, Pavlenko EV, Sedov AV, Zaitsev AYu, Aleksandrova SA, Zaklyaz’minskaya EV. COVID-19 in patients with primary cardiomyopathies: clinical course and outcomes, role of verified post-COVID myocarditis. Clinical and Experimental Surgery. Petrovsky Journal. 2024;12(2):71-80. (In Russ.). https://doi.org/10.33029/2308-1198-2024-12-2-71-80
  24. Normandin E, Rudy M, Barkas N, Schaffner SF, Levine Z, Padera RF Jr, Babadi M, Mukerji SS, Park DJ, MacInnis BL, et al. High-depth sequencing characterization of viral dynamics across tissues in fatal COVID-19 reveals compartmentalized infection. Nat Commun. 2023;14(1):574.  https://doi.org/10.1038/s41467-022-34256-y
  25. Mfouo-Tynga IS, Mouinga-Ondeme AG. Photodynamic therapy: a prospective therapeutic approach for viral infections and induced neoplasia. Pharmaceuticals (Basel). 2022;15(10):1273. https://doi.org/10.3390/ph15101273
  26. Sokolov VV, Filonenko EV. Photodynamic therapy in patients with early central lung cancer. Photodynamic Therapy and Photodiagnosis. 2013;2(4):3-6. (In Russ.).
  27. Shafirstein G, Battoo A, Harris K, Baumann H, Gollnick SO, Lindenmann J, Nwogu CE. Photodynamic therapy of non-small cell lung cancer. Narrative review and future directions. Ann Am Thorac Soc. 2016;13(2):265-275.  https://doi.org/10.1513/AnnalsATS.201509-650FR
  28. Kogan E, Meerovich G, Karshieva S, Makarova E, Romanishkin I, Akhlyustina E, Meerovich I, Zharkov N, Kharnas S, Levkin V, et al. Polycationic photosensitizers as effective anticancer agents that destroy cancer stem cells, cancer vascularization and induce protective desmoplastic reaction around lung cancers. Photonics. 2024;11(6),485.  https://doi.org/10.3390/photonics11060485
  29. Svyatchenko VA, Nikonov SD, Mayorov AP, Gelfond ML, Loktev VB. Antiviral photodynamic therapy: inactivation and inhibition of SARS-CoV-2 in vitro using methylene blue and Radachlorin. Photodiagnosis Photodyn Ther. 2021;33:102112. https://doi.org/10.1016/j.pdpdt.2020.102112
  30. Emadi E, Hamidi Alamdari D, Attaran D, Attaran S. Application of methylene blue for the prevention and treatment of COVID-19: a narrative review. Iran J Basic Med Sci. 2024;27(7):780-792.  https://doi.org/10.22038/IJBMS.2024.71871.15617
  31. Zhukhovitsky V, Shevlyagina N, Zubasheva M, Russu L, Gushchin V, Meerovich G, Strakhovskaya M. Infectivity and morphology of bovine coronavirus inactivated in vitro by cationic photosensitizers. Viruses. 2022;14(5):1053. https://doi.org/10.3390/v14051053
  32. Sharshov K, Solomatina M, Kurskaya O, Kovalenko I, Kholina E, Fedorov V, Meerovich G, Rubin A, Strakhovskaya M. The photosensitizer octakis(cholinyl)zinc phthalocyanine with ability to bind to a model spike protein leads to a loss of SARS-CoV-2 infectivity in vitro when exposed to far-red LED. Viruses. 2021;13(4):643.  https://doi.org/10.3390/v13040643
  33. COVID-19 treatment using methylene blue and photodynamic therapy. ClinicalTrials.gov. ID NCT04933864. 2021. Available at: https://clinicaltrials.gov/study/NCT04933864

Close metadata

Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register


We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.