The Specific Features of the Inflammatory Process Accompanying Ligation of the Femoral Vein in Experiment Under the Conditions of Stem Cell Therapy

Maĭborodin I.V.; Elovskiy A.A.; Mikheeva T.V.; Figurenko N.F.; Maslov R.V.; Maiborodina V.I.; Shevela A.I.; Anishchenko V.V.; Kuzkin S.A.

doi:https://doi.org/10.17116/flebo201812041270

Maĭborodin I.V.

NII klinicheskoĭ i éksperimental'noĭ limfologii SO RAMN, Novosibirsk

Elovskiy A.A.

JSC «Medical Center «Avicenna», Novosibirsk, Russia

Mikheeva T.V.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Figurenko N.F.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Maslov R.V.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Maiborodina V.I.

Laboratory of ultrastructural bases of pathology, Institute of Molecular Pathology and Pathomorphology, Federal State Budget Scientific Institution «Federal Research Center of Fundamental and Translational Medicine», Novosibirsk, Russia

Shevela A.I.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

ORCID: 0000-0002-3164-9377

Anishchenko V.V.

Novosibirskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Kuzkin S.A.

Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia

The Specific Features of the Inflammatory Process Accompanying Ligation of the Femoral Vein in Experiment Under the Conditions of Stem Cell Therapy

Authors:

Maĭborodin I.V., Elovskiy A.A., Mikheeva T.V., Figurenko N.F., Maslov R.V., Maiborodina V.I., Shevela A.I., Anishchenko V.V., Kuzkin S.A.

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Journal: Journal of Venous Disorders. 2018;12(4): 270‑279

DOI: 10.17116/flebo201812041270

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To cite this article:

Maĭborodin IV, Elovskiy AA, Mikheeva TV, et al. . The Specific Features of the Inflammatory Process Accompanying Ligation of the Femoral Vein in Experiment Under the Conditions of Stem Cell Therapy. Journal of Venous Disorders. 2018;12(4):270‑279. (In Russ.)
https://doi.org/10.17116/flebo201812041270

Подписка 2026 Флебология (Journal of Venous Disorders)

Использование инфографики авторами научных работ

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Abstract:

Aim — the objective of the present work was to study the inflammatory process in the rat tissues after ligation of the femoral vein and the administration of autologic multi-potent mesenchymal stromal cells of the bone marrow origin (MMSC). Material and methods. The inflammatory process developing after ligation of the femoral vein in 224 male rats of the Wag line was studied by light microscopy following an autologic MMSC injection. Results. Diffuse leukocyte infiltration of the tissues with the predominance of lymphocytes and macrophages in the animal model of acute local blockade of the main vein, is most possibly caused by surgical intervention. Nonetheless, we found a significant amount of absorbable suture material used for ligation in the femoral vein even 5 weeks after the intervention. The multifilamentous sutural fragments have been encapsulated. Smaller parts of the fibers have been also surrounded by the connective tissue with a large number of macrophages including gigantic multi-nuclear forms with fused cytoplasm. Phagocytes together with fibroblasts encloed separate filaments of the thread, alienating them from each other, thus dividing the multi-filamentous thread. The presence of the slowly absorbed sutural material in the tissues after the interventions on the vessels seems to be the main reason for the development of long-standing aseptic granulomatous inflammation. On the 3rd week after the administration of MMSC, the number of leukocytes in the tissues under conditions of a femoral venous blockade normalized, while in the absence of MMSC the amount of leukocytes in the tissue returned to the normalized level on only by the fourth week. Moreover, the application of MMSC led to the 3.6-fold reduction of tissue infiltration by leukocytes in 2 weeks and the 4.8-fold reduction in numerical density of neutrophils. Conclusion. The attenuation of the inflammatory process after vein ligation is attributable to the immuomodulatory action of the injected autologic multi-potent mesenchymal stromal cells of the bone marrow origin.

Keywords:

multi-potent mesenchymal stromal cells

inflammation

leukocytes

neutrophils

gigantic cells of foreign bodies

Authors:

Maĭborodin I.V.

NII klinicheskoĭ i éksperimental'noĭ limfologii SO RAMN, Novosibirsk

Elovskiy A.A.

JSC «Medical Center «Avicenna», Novosibirsk, Russia

Mikheeva T.V.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Figurenko N.F.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Maslov R.V.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

Maiborodina V.I.

Shevela A.I.

The Center of New Medical Technologies Institute of Chemical Biology and Fundamental Medicine, The Russian Academy of Sciences, Siberian Branch Novosibirsk, Russia

ORCID: 0000-0002-3164-9377

Anishchenko V.V.

Novosibirskiĭ gosudarstvennyĭ meditsinskiĭ universitet

Kuzkin S.A.

Laboratory of Ultrastructural Bases of Pathology, Institute of Molecular Pathology and Pathomorphology, Novosibirsk, Russia

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References:

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