ABSTRACT Goal: to assess the development of a systemic inflammatory response, as well as to determine intraoperative circulating DNA levels in plasma of patients with intracranial meningiomas during surgery under sevoflurane anesthesia combined with fentanyl. Methods. The study included fourteen patients with intracranial meningioma. Control group contained five healthy adults. Patients with meningioma were operated under sevoflurane anesthesia combined with fentanyl. Samples of plasma were collected intraoperatively at three time points: (1) 30 minutes after an induction of anesthesia before the surgical incision, (2) after opening the dura mater before removal of the tumor, (3) after removal of the tumor and suturing the wound. The concentrations of protein markers were determined by the immunoenzyme assay. Analysis of nucleic acid levels was performed using real-time polymerase chain reaction. Results. During the surgery, no statistically significant changes in the concentrations of proinflammatory cytokines IL-6 and IL-8 were detected in the plasma of patients with meningiomas. Before the onset of general anesthesia and surgery, the levels of mitochondrial DNA and nuclear DNA in healthy adults and patients with meningioma did not differ. The level of mitochondrial DNA did not change during the intervention. At the stage of tumor removal, an increase in the level of nuclear DNA was observed (9.2-fold; p=0.043). Conclusion. Patients with intracranial meningioma, operated under general anesthesia with sevoflurane combined with fentanyl showed no biochemical markers characteristic of a pronounced systemic inflammatory response. However, the stage of removal of meningioma was associated with an increase in plasma levels of nuclear, but not mitochondrial DNA.