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Gromova O.A.

Rossiĭskiĭ satellitnyĭ tsentr instituta mikroélementov IuNESKO;
Laboratoriia vychislitel'noĭ i sistemnoĭ biologii VTs RAN im. A.A. Dorodnitsyna;
kafedra farmakologii i klinicheskoĭ farmakologii Ivanovskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Torshin I.Iu.

Rossiĭskiĭ satellitnyĭ tsentr instituta mikroélementov IuNESKO;
Laboratoriia vychislitel'noĭ i sistemnoĭ biologii VTs RAN im. A.A. Dorodnitsyna

Zgoda V.G.

Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

Tikhonova O.V.

Endocrinology Research Centre, Moscow, Russia

An analysis of the peptide composition of a 'light' peptide fraction of cerebrolysin

Authors:

Gromova O.A., Torshin I.Iu., Zgoda V.G., Tikhonova O.V.

More about the authors

Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2019;119(8): 75‑83

DOI: 10.17116/jnevro201911908175

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Table of contents

AN ANALYSIS OF THE PEPTIDE COMPOSITION OF A 'LIGHT' PEPTIDE FRACTION OF CEREBROLYSIN
AN ANALYSIS OF THE PEPTIDE COMPOSITION OF A 'LIGHT' PEPTIDE FRACTION OF CEREBROLYSIN

To cite this article:

Gromova OA, Torshin IIu, Zgoda VG, Tikhonova OV. An analysis of the peptide composition of a 'light' peptide fraction of cerebrolysin. S.S. Korsakov Journal of Neurology and Psychiatry. 2019;119(8):75‑83. (In Russ., In Engl.)
https://doi.org/10.17116/jnevro201911908175

Подписка 2026 Журнал неврологии и психиатрии им. С.С. Корсакова (S.S. Korsakov Journal of Neurology and Psychiatry)
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Abstract:

Objective. To analyze the peptide composition of a light peptide fraction of cerebrolysin. Material and methods. Mass spectrometry (MS) with orbital ion traps and modern de novo MS-sequencing algorithms was performed. Results. The amino acid sequences of 14 635 peptides corresponding to the 1643 porcine proteome neuronal proteins are identified. An analysis of the human proteome annotation shows that these peptides can mimic the corresponding human peptides. In particular, 405 peptide fragments correspond to 300 known biologically active peptides, including fragments of antibacterial peptides (defensins, histatins), immunomodulatory (granulin, manserin) and vasoactive (endothelin, VIP) peptides. At the same time, 8953 of 14 635 peptides can modulate the activity of 275 human signaling proteins, including kinases CDK1, CDK2, TGFBR2, GSK3, MTOR, pro-apoptotic caspases CASP1, CASP3 and CASP6 etc. The results confirm the presence of Leu- and Met-enkephalins, fragments of neuropeptide orexin, neuropeptide VF, galanin and nerve growth factor that have a neurotrophic effect. Conclusion. The results of a proteomic study of the peptide composition of cerebrolysin indicate the widest range of molecular mechanisms responsible for the clinical efficacy of this drug.

Keywords:

cerebrolysin
proteomics
mass-spectrometry
de novo sequencing
algorithms for big data analysis

Authors:

Gromova O.A.

Rossiĭskiĭ satellitnyĭ tsentr instituta mikroélementov IuNESKO;
Laboratoriia vychislitel'noĭ i sistemnoĭ biologii VTs RAN im. A.A. Dorodnitsyna;
kafedra farmakologii i klinicheskoĭ farmakologii Ivanovskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Torshin I.Iu.

Rossiĭskiĭ satellitnyĭ tsentr instituta mikroélementov IuNESKO;
Laboratoriia vychislitel'noĭ i sistemnoĭ biologii VTs RAN im. A.A. Dorodnitsyna

Zgoda V.G.

Orekhovich Research Institute of Biomedical Chemistry, Moscow, Russia

Tikhonova O.V.

Endocrinology Research Centre, Moscow, Russia

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References:

  1. Selezneva ND, Roshchina IF, Korovaitseva GI, Gavrilova SI. Prevention of progression of cognitive decline in the first-degree relatives of patients with Alzheimer’s disease. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2018;118(10):30-36. (In Russ.) https://doi.org/10.17116/jnevro201811810130
  2. Dong HY, Jiang XM, Niu CB, Du L, Feng JY, Jia FY. Cerebrolysin improves sciatic nerve dysfunction in a mouse model of diabetic peripheral neuropathy. Neural Regen Res. 2016;11(1):156-162. https://doi.org/10.4103/1673-5374.175063
  3. Zhang L, Chopp M, Lu M, Zhang T, Li C, Winter S, Brandstaetter H, Doppler E, Meier D, Pabla P, Zhang ZG. Demonstration of therapeutic window of Cerebrolysin in embolic stroke: A prospective, randomized, blinded, and placebo-controlled study. Int J Stroke. 2017;12(6):628-635. https://doi.org/10.1177/1747493017702665
  4. Alzoubi KH, Al-Ibbini AM, Nuseir KQ. Prevention of memory impairment induced by post-traumatic stress disorder by cerebrolysin. Psychiatry Res. 2018;270:430-437. https://doi.org/10.1016/j.psychres.2018.10.008
  5. Ghavimi H, Darvishi S, Ghanbarzadeh S. Attenuation of Morphine-Induced Tolerance and Dependence by Pretreatment with Cerebrolysin in Male rats. Drug Res (Stuttg). 2018;68(1):33-37. https://doi.org/10.1055/s-0043-116948
  6. Mahmoudi J, Mohaddes G, Erfani M, Sadigh-Eteghad S, Karimi P, Rajabi M, Reyhani-Rad S, Farajdokht F. Cerebrolysin attenuates hyperalgesia, photophobia, and neuroinflammation in a nitroglycerin-induced migraine model in rats. Brain Res Bull. 2018;140:197-204. https://doi.org/10.1016/j.brainresbull.2018.05.008
  7. Gromova OA, Kalacheva AG, Grishina TR, Bogacheva TE, Demidov VI, Torshin IYu. Neurotrophic peptides of small es, Cyrillicerebrolysin as a basis for anticonvulsant effect of the drug. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2016;116(3):55-62. (In Russ.) https://doi.org/10.17116/jnevro20161163155-62
  8. Gromova OA, Pronin AV, Torshin IYu, Kalacheva AG, Filimonova MV, Demidov VI, Gogoleva IV, Grishina TR. Evaluation of the antitumor potential of cerebrolysin. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2016;116(11):69-77. (In Russ.) https://doi.org/10.17116/jnevro201611611169-77
  9. Stan A, Birle C, Blesneag A, Iancu M. Cerebrolysin and early neurorehabilitation in patients with acute ischemic stroke: a prospective, randomized, placebo-controlled clinical study. J Med Life. 2017;10(4):216-222.
  10. Park YK, Yi HJ, Choi KS, Lee YJ, Kim DW, Kwon SM. Cerebrolysin for the Treatment of Aneurysmal Subarachnoid Hemorrhage in Adults: A Retrospective Chart Review. Adv Ther. 2018;35(12):2224-2235. https://doi.org/10.1007/s12325-018-0832-8
  11. Malashenkova IK, Krynskiy SA, Hailov NA, Ogurtsov DP, Selezneva ND, Fedorova YaB, Ponomareva EV, Kolyhalov IV, Gavrilova SI, Didkovsky NA. Anti-inflammatory effects of neurotrophic therapy (a pilot study). Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2018;118(5):39-44. (In Russ.) https://doi.org/10.17116/jnevro20181185139
  12. Chutko LS, Yakovenko EA, Surushkina SYu, Kryukova EM, Palaieva SV. The efficacy of cerebrolysin in the treatment of autism spectrum disorders. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2017;117(9):71-75. (In Russ.) https://doi.org/10.17116/jnevro20171179171-75
  13. Serkina EV, Gromova OA, Torshin IYu, Sotnikova NYu, Nikonov AA. Cerebrolysin alleviates perinatal CNS disorders through the autoimmune modulation and antioxidant protection. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2008;108(11):62-66. (In Russ.)
  14. Bornstein NM, Guekht A, Vester J, Heiss WD, Gusev E, Homberg V, Rahlfs VW, Bajenaru O, Popescu BO, Muresanu D. Safety and efficacy of Cerebrolysin in early post-stroke recovery: a meta-analysis of nine randomized clinical trials. Neurol Sci. 2018;39(4):629-640. https://doi.org/10.1007/s10072-017-3214-0
  15. Gauthier S, Proano JV, Jia J, Froelich L, Vester JC, Doppler E. Cerebrolysin in mild-to-moderate Alzheimer’s disease: a meta-analysis of randomized controlled clinical trials. Dement Geriatr Cogn Disord. 2015;39(5-6):332-347. https://doi.org/10.1159/000377672
  16. Ghaffarpasand F, Torabi S, Rasti A, Niakan MH, Aghabaklou S, Pakzad F, Beheshtian MS, Tabrizi R. Effects of cerebrolysin on functional outcome of patients with traumatic brain injury: a systematic review and meta-analysis. Neuropsychiatr Dis Treat. 2018;15:127-135. https://doi.org/10.2147/NDT.S186865
  17. Zhang L, Chopp M, Wang C, Zhang Y, Lu M, Zhang T, Zhang ZG. Prospective, double blinded, comparative assessment of the pharmacological activity of Cerebrolysin and distinct peptide preparations for the treatment of embolic stroke. J Neurol Sci. 2019;398:22-26. https://doi.org/10.1016/j.jns.2019.01.017
  18. Stepanichev M, Onufriev M, Aniol V, Freiman S, Brandstaetter H, Winter S, Lazareva N, Guekht A, Gulyaeva N. Effects of cerebrolysin on nerve growth factor system in the aging rat brain. Restor Neurol Neurosci. 2017;35(6):571-581. https://doi.org/10.3233/RNN-170724. PMID:29172008
  19. Gromova OA, Torshin IYu, Gogoleva IV, Pronin AV, Stelmashuk EV, Isaev NK, Genrikhs EE, Demidov VI, Volkov AYu, Khaspekov GL, Alexandrova OP. Pharmacokinetic and pharmacodynamic synergism between neuropeptides and lithium in the neurotrophic and neuroprotective action of cerebrolysin. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2015;115(3):65-72. (In Russ.) https://doi.org/10.17116/jnevro20151153165-72
  20. Gromova OA, Tret’iakov VE, Moshkovskii SA, Gusev EI, Nikonov AA, Val’kova LA, Glibin AS, Kataev AS. An oligopeptide membrane fraction of cerebrolysin. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2006;106(7):68-70. (In Russ.)
  21. Gromova OA, Torshin IYu, Gogoleva IV. Mechanisms of neurotrophic and neuroprotective effects of cerebrolysin in cerebral ischemia. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2014;114(3 Pt 2):43-50. (In Russ.)
  22. Gevaert B, D’Hondt M, Bracke N, Yao H, Wynendaele E, Vissers JP, De Cecco M, Claereboudt J, De Spiegeleer B. Peptide profiling of Internet-obtained Cerebrolysin using high performance liquid chromatography — electrospray ionization ion trap and ultra high performance liquid chromatography — ion mobility — quadrupole time of flight mass spectrometry. Drug Test Anal. 2015;7(9):835-842. https://doi.org/10.1002/dta.1817
  23. Keits M. Tekhnika lipidologii. M.: Mir; 1975. (In Russ.)
  24. Darbre A. Prakticheskaya khimiya belka. M.: Mir; 1989. (In Russ.)
  25. Wells JM, McLuckey SA. Collision-induced dissociation (CID) of peptides and proteins. Meth Enzymol Methods in Enzymology. 2005;402:148-185. https://doi.org/10.1016/S0076-6879(05)02005-7
  26. Frank AM. Predicting intensity ranks of peptide fragment ions. J Proteome Res. 2009;8(5):2226-2240. https://doi.org/10.1021/pr800677f
  27. Torshin IYu, Rudakov KV. On the theoretical basis of metric analysis of poorly formalized problems of recognition and classification. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2015;25(4):577-587.
  28. Torshin IYu, Rudakov KV. On metric spaces arising during formalization of problems of recognition and classification. part 1: properties of compactness. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2016;26(2):274-284.
  29. Torshin IYu, Rudakov KV. On metric spaces arising during formalization of problems of recognition and classification. part 2: density properties. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2016;26(3):483-496.
  30. Torshin IYu. The study of the solvability of the genome annotation problem on sets of elementary motifs. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2011;21:4:652-662.
  31. Torshin IYu, Rudakov KV. Combinatorial analysis of the solvability properties of the problems of recognition and completeness of algorithmic models. part 1: factorization approach. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2017;27(1):16-28.
  32. Torshin IYu, Rudakov KV. Combinatorial analysis of the solvability properties of the problems of recognition and completeness of algorithmic models. part 2: metric approach within the framework of the theory of classification of feature values. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2017;27(2):184-199.
  33. Torshin IYu, Rudakov KV. On the application of the combinatorial theory of solvability to the analysis of chemographs. part 1: fundamentals of modern chemical bonding theory and the concept of the chemograph. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2014;24:1:11-23.
  34. Torshin IYu, Rudakov KV. On the application of the combinatorial theory of solvability to the analysis of chemographs: part 2. local completeness of invariants of chemographs in view of the combinatorial theory of solvability. Pattern Recognition and Image Analysis (Advances in Mathematical Theory and Applications). 2014;24:2:196-208.
  35. Torshin IYu, Gromova OA, Sardaryan IS, Fedotova LE. A comparative chemoreactome analysis of mexidol. Zhurnal Nevrologii i Psihiatrii im. S.S. Korsakova. 2017;117(1. Vyp. 2):75-83. (In Russ.) https://doi.org/10.17116/jnevro20171171275-84.
  36. UniProt Consortium. UniProt: a worldwide hub of protein knowledge. Nucleic Acids Res. 2019;8:47(D1):506-515. PMID:30395287. https://doi.org/10.1093/nar/gky1049
  37. Colangelo AM, Bianco MR, Vitagliano L, Cavaliere C, Cirillo G, De Gioia L, Diana D, Colombo D, Redaelli C, Zaccaro L, Morelli G, Papa M, Sarmientos P, Alberghina L, Martegani E. A new nerve growth factor-mimetic peptide active on neuropathic pain in rats. J Neurosci. 2008;28(11):2698-2709. https://doi.org/10.1523/JNEUROSCI.5201-07.2008
  38. Yamada N, Katsuura G, Tatsuno I, Kawahara S, Ebihara K, Saito Y, Nakao K. Orexins increase mRNA expressions of neurotrophin-3 in rat primary cortical neuron cultures. Neurosci Lett. 2009;450(2):132-135. https://doi.org/10.1016/j.neulet.2008.11.028
  39. Vrontakis ME. Galanin: a biologically active peptide. Curr Drug Targets CNS Neurol Disord. 2002;1(6):531-541.
  40. Suarez V, Guntinas-Lichius O, Streppel M, Ingorokva S, Grosheva M, Neiss WF, Angelov DN, Klimaschewski L. The axotomy-induced neuropeptides galanin and pituitary adenylate cyclase-activating peptide promote axonal sprouting of primary afferent and cranial motor neurones. Eur J Neurosci. 2006;24(6):1555-1564. https://doi.org/10.1111/j.1460-9568.2006.05029.x
  41. Toshinai K, Nakazato M. Neuroendocrine regulatory peptide-1 and -2: novel bioactive peptides processed from VGF. Cell Mol Life Sci. 2009;66(11-12):1939-1945. https://doi.org/10.1007/s00018-009-8796-0
  42. Vadnal J. NeuroPeptide K. In book: Reference Module in Biomedical Sciences, xPharm: The Comprehensive Pharmacology Reference. 2007;1-5. https://doi.org/10.1016/B978-0-12-801238-3.99363-2
  43. Jensen RT, Battey JF, Spindel ER, Benya RV. International Union of Pharmacology. LXVIII. Mammalian bombesin receptors: nomenclature, distribution, pharmacology, signaling, and functions in normal and disease states. Pharmacol Rev. 2008;60(1):1-42. https://doi.org/10.1124/pr.107.07108
  44. Sakamoto T, Mori K, Miyazato M, Kangawa K, Sameshima H, Nakahara K, Murakami N. Involvement of neuromedin S in the oxytocin release response to suckling stimulus. Biochem Biophys Res Commun. 2008;375(1):49-53. https://doi.org/10.1016/j.bbrc.2008.07.124
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