Objective. To study the association between ischemic stroke (IS) and rs11575945 polymorphism of gene encoding an apoptotic marker annexin-A5 protein (ANXV), to evaluate the serum content of annexin-A5 protein in patients with IS on the first day of stroke compared to healthy subjects and the relationship between the levels of annexin-A5 protein and rs11575945 polymorphism. Material and methods. The study included 94 patients in the acute stage of IS. Polymerase chain reaction with sequence-specific primers and enzyme-linked immunosorbent assay were used. Results and conclusion. ANXV mutant allele frequency was in average 2.6 times higher in patients (p<0.00001) than in healthy subjects, and annexin-A5 mean concentration in the blood serum of IS patients was 3 times higher compared to healthy subjects. Moreover, the concentration of annexin-A5 in the serum of the rs11575945*T mutant allele carriers of the ANXV gene was 4 times higher in comparison to individuals homozygous for the rs11575945C standard allele.