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Eremin I.I.

Petrovsky National Research Center of Surgery

Briko A.N.

Petrovsky National Research Center of Surgery

Kotenko K.V.

Petrovsky National Research Center of Surgery

Standardized and scalable method for stromal-vascular fraction harvesting from adipose tissue

Authors:

Eremin I.I., Briko A.N., Kotenko K.V.

More about the authors

Journal: Regenerative Biotechnologies, Preventive, Digital and Predictive Medicine. 2024;1(4): 76‑81

DOI: 10.17116/rbpdpm2024104176

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Table of contents

STANDARDIZED AND SCALABLE METHOD FOR STROMAL-VASCULAR FRACTION HARVESTING FROM ADIPOSE TISSUE
STANDARDIZED AND SCALABLE METHOD FOR STROMAL-VASCULAR FRACTION HARVESTING FROM ADIPOSE TISSUE

To cite this article:

Eremin II, Briko AN, Kotenko KV. Standardized and scalable method for stromal-vascular fraction harvesting from adipose tissue. Regenerative Biotechnologies, Preventive, Digital and Predictive Medicine. 2024;1(4):76‑81. (In Russ.)
https://doi.org/10.17116/rbpdpm2024104176

Подписка 2026 Восстановительные биотехнологии, профилактическая, цифровая и предиктивная медицина (Regenerative Biotechnologies, Preventive, Digital and Predictive Medicine)
МСФ на ЯМ
Использование инфографики авторами научных работ
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Abstract:

OBJECTIVE

To develop a standardized and scalable method for stromal-vascular fraction harvesting from adipose tissue.

MATERIAL AND METHODS

We describe a standardized and scalable method for SVF harvesting from adipose tissue. These methods are aimed at improving the reproducibility of results and ensuring the safety of cellular product.

RESULTS

The proposed method allows SVF harvesting in the operating theatre that minimizes the period between lipoaspirate collection and injection.

CONCLUSION

Standardization of SVF harvesting processes contributes to its wider application in clinical practice, increases the effectiveness of cell therapy and improves treatment prognosis.

Keywords:

stromal-vascular fraction
adipose tissue
regenerative medicine
cell therapy
standardization
lipoaspiration
technique

Authors:

Eremin I.I.

Petrovsky National Research Center of Surgery

Briko A.N.

Petrovsky National Research Center of Surgery

Kotenko K.V.

Petrovsky National Research Center of Surgery

Received:

18.09.2024

Accepted:

01.10.2024

Close metadata

References:

  1. Zuk PA, Zhu M, Mizuno H, Huang J, Futrell JW, Katz AJ, Benhaim P, Lorenz HP, Hedrick MH. Multilineage cells from human adipose tissue: implications for cell-based therapies. Tissue Engineering. 2001;7(2):211-228.  https://doi.org/10.1089/107632701300062859
  2. Gimble JM, Katz AJ, Bunnell BA. Adipose-derived stem cells for regenerative medicine. Annual Review of Biomedical Engineering. 2011;13:27-47. 
  3. Fraser JK, Wulur I, Alfonso Z, Hedrick MH. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends in Biotechnology. 2006;24(4):150-154.  https://doi.org/10.1016/j.tibtech.2006.01.010
  4. Bianchi F, et al. Lipogems®: a new modality of fat tissue handling to enhance tissue repair. The International Journal of Stem Cells. 2013;6(1):184-194. 
  5. Prizov A, Tchetina E, Volkov A, Eremin I, Zagorodniy N, Lazko F, Pulin A, Belyak E, Kotenko K, Eshmotova G, Glukhova S, Lila A. Long-term structural changes in the osteochondral unit in patients with osteoarthritis undergoing corrective osteotomy with platelet-rich plasma or stromal vascular fraction post-treatment. Biomedicines. 2024;12(5):1044. https://doi.org/10.3390/biomedicines12051044
  6. Fraser JK, Wulur I, Alfonso Z, Hedrick MH. Fat tissue: an underappreciated source of stem cells for biotechnology. Trends in Biotechnology. 2006;24(4):150-154.  https://doi.org/10.1016/j.tibtech.2006.01.010
  7. Tiryaki T, Cohen SR, Canikyan Turkay S, Kocak P, Sterodimas A, Schlaudraff KU, Akgün Demir I, Agovino A, Kul Y. Hybrid stromal vascular fraction (Hybrid-SVF): A new paradigm in mechanical regenerative cell processing. Plastic and Reconstructive Surgery Global Open. 2022;10(12):4702. https://doi.org/10.1097/GOX.0000000000004702
  8. Vasiliev VS, Korchazhkina NB, Mikhailova AA, Nadelyaeva II, Vasiliev SA, Vasiliev IS, Vasiliev YuS, Teryushkova ZhI, Kazantsev IB, Vasilyeva ES, Kotenko KV. Modern technologies of regenerative rehabilitation in the treatment of patients with pathological scar changes. Pirogov Russian Journal of Surgery. 2021;6-2:7-14. (In Russ.). https://doi.org/10.17116/hirurgia20210627
  9. Kotenko KV, Eremin II, Moroz BB, Bushmanov AYu, Nadezhina NM, Galstyan IA, Grinakovskaya OS, Aksenenko AV, Deshevoy YuB, Lebedev VG, Slobodina TS, Zhgutov YuA, Lauk-Dubitsky SE, Eremin PS. Cellular technologies in the treatment of radiation burns: the experience of the A.I. Burnazyan Federal Medical and Biochemical Center. Kletochnaya transplantologiya i tkanevaya inzheneriya. 2012;7(2):97-102. (In Russ.).
  10. Smirnov SV, Kiselev IV, Vasiliev AV, Terskikh VV. Modern methods of cell therapy in the treatment of burns. Pirogov Russian Journal of Surgery. 2003;(12):58. (In Russ.).
  11. Jiang S, Quan Y, Wang J, Cai J, Lu F. Fat grafting for facial rejuvenation using stromal vascular fraction gel injection. Clinics in Plastic Surgery. 2020;47(1):73-79.  https://doi.org/10.1016/j.cps.2019.09.001
  12. Goncharov EN, Koval OA, Nikolaevich Bezuglov E, Encarnacion Ramirez MJ, Engelgard M, Igorevich EI, Saporiti A, Valentinovich Kotenko K, Montemurro N. Stromal vascular fraction therapy for knee osteoarthritis systematic review. Medicina (Kaunas, Lithuania). 2023;59(12):2090. https://doi.org/10.3390/medicina59122090
  13. Briko AN, Tihomirov AN, Hammood AM, Dmitriev AN, Kapravchuk VV, Kobelev AV, Shchukin SI, Kotenko KV, Eremin II. Biomechanical aspects of designing a device for mechanical production of stromal-vascular fraction of adipose tissue. Meditsinskaya tekhnika. 2023;5(341):50-53. (In Russ.).
  14. Briko AN, Tikhomirov AN, Hammoud AM, Dmitriev AN, Kapravchuk VV, Kobelev AV, Shchukin SI, Kotenko KV, Eremin II. Biomechanical aspects of the design of a device for mechanical extraction of the stromal vascular fraction of adipose tissue. Biomedical Engineering. 2024;57(5):362-366.  https://doi.org/10.1007/s10527-023-10335-2
  15. Eremin II, Vasilyev VS, Gurba MA, Briko AN, Kotenko KV. Stromal vascular fraction isolation methods. Literature review. Plastic Surgery and Aesthetic Medicine. 2023;(4-2):68-75. (In Russ.). https://doi.org/10.17116/plast.hirurgia202304268
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