Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay

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Gusarov A.A.

th Main State Centre for Forensic Medical and Criminalistic Examinations;
Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medical Expertise;
S.M. Kirov Medical Military Academy

ORCID: 0000-0001-6588-1898

Nagornov M.N.

Russian Center of Forensic Medical Expertise

ORCID: 0000-0001-5077-2090

Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay

Authors:

Gusarov A.A., Sidorov V.L., Yagmurov O.D., Nagornov M.N.

More about the authors

Journal: Forensic Medical Expertise. 2025;68(1): 38‑42

DOI: 10.17116/sudmed20256801138

Views: 363

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To cite this article:

Gusarov AA, Sidorov VL, Yagmurov OD, Nagornov MN. Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025;68(1):38‑42. (In Russ.)
https://doi.org/10.17116/sudmed20256801138

Подписка 2025-2 (Forensic Medical Expertise)

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The objective of the study is to develop the methodology for conducting intralaboratory quality control of total IgG investigations in establishing species of the blood by quantitative enzyme-linked immunosorbent assay (ELISA) using the Russian «IgG-total-ELISA-BEST» kit. A sequential 3-step algorithm presented by the article’s authors can be used by experts of forensic biological units in carrying out quantitative ELISA to reveal possible errors that could invalidate the obtained results.

Keywords:

quantitative enzyme-linked immunosorbent assay

total immunoglobulin G

forensic medical expertise

intralaboratory control of investigations quality

blood

species membership

Authors:

Gusarov A.A.

th Main State Centre for Forensic Medical and Criminalistic Examinations;
Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medical Expertise;
S.M. Kirov Medical Military Academy

ORCID: 0000-0001-6588-1898

Nagornov M.N.

Russian Center of Forensic Medical Expertise

ORCID: 0000-0001-5077-2090

Received:

06.03.2024

Accepted:

11.03.2024

List of references:

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