Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay

Gusarov A.A.; Sidorov V.L.; Yagmurov O.D.; Nagornov M.N.

doi:https://doi.org/10.17116/sudmed20256801138

Gusarov A.A.

th Main State Centre for Forensic Medical and Criminalistic Examinations;
Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Yagmurov O.D.

Bureau of Forensic Medical Expertise;
S.M. Kirov Medical Military Academy

ORCID: 0000-0001-6588-1898

Nagornov M.N.

Russian Center of Forensic Medical Expertise

ORCID: 0000-0001-5077-2090

Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay

Authors:

Gusarov A.A., Sidorov V.L., Yagmurov O.D., Nagornov M.N.

More about the authors

Journal: Forensic Medical Expertise. 2025;68(1): 38‑42

DOI: 10.17116/sudmed20256801138

Read: 892 times

Contact the author

Table of contents

To cite this article:

Gusarov AA, Sidorov VL, Yagmurov OD, Nagornov MN. Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025;68(1):38‑42. (In Russ.)
https://doi.org/10.17116/sudmed20256801138

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

References:

Salagay OO, Letnikova LI, Nekrasov MS, Makarov IYu, Minaeva PV. Increased quality and accessibility of all types of forensic examinations as a result of the implementation of the «Modern model of organization and performance of forensic examinations in the Russian Federation». Forensic Medical Expertise. 2021;64(6):4-7. (In Russ.). https://doi.org/10.17116/sudmed2021640614
Barinov EKh, Kalinin RE, Kovalev AV. Role and importance of forensic medical re-examination of defects in health care delivery. Forensic Medical Expertise. 2023;66(5):5-10. (In Russ.). https://doi.org/10.17116/sudmed2023660515
Rasporyazhenie Pravitel’stva Rossiyskoy Federatsii ot 27.12.19 №3225-r «Ob utverzhdenii plana meropriyatiy («dorozhnoy karty») po sovershenstvovaniyu organizatsii i proizvodstva sudebno-meditsinskikh i sudebno-psikhiatricheskikh ekspertiz v Rossijskoy Federatsii». (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Khorovskaya LA, Portnova NA. Intraboratory quality control of α-amylase concentration measurements when establishing the presence of saliva on physical evidence using the colorimetric method. Vestnik sudebnoy meditsiny. 2022;11(1):14-19. (In Russ.).
Zimina YuV. Opredelenie gruppovyh faktorov izoserologicheskoj sistemy AB0 v sledah krovi maloj velichiny reakciej absorbcii-elyucii: uchebnoe posobie dlya vrachej. Sankt-Peterburg: SGZMU im. M.I. Mechnikova. 2012. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Khorovskaya LA. Application of the reproductibility control method for in-laboratory quality control during the establishment of the concentration of total PSA and total IGG in blood and sperm samples. Vestnik sudebnoy meditsiny. 2022;11(2);23-28. (In Russ.).
Tamaki Y, Kishida T, Nishimukai H. Identification of human blood with hybridoma-derived antibody to human immunoglobulin G. J. Forensic Science. 1984;29(3):885-888.
Sidorov VL, Yagmurov OD, Gusarov AA, Khorovskaya LA. Intra laboratory quality control when establishing total IGG concentration in aquatic extracts from blood spots. Vestnik sudebnoy meditsiny. 2022;11(3):27-33. (In Russ.).
Clarke WL, Cox D, Gonder-Frederick LA. Evaluating clinical accuracy of systems for self-monitoring of blood glucose. Diabetes Care. 1987;2(2):622-628.
Sidorov VL, Gusarov AA, Surikova NE, Khorovskaya LA, Loban IE. Ability to evaluate the intralaboratory quality control of establishing the concentration of the total PSA in water extracts from the semen stains on physical evidence. Vestnik sudebnoy meditsiny. 2019;8(3):20-27. (In Russ.).
Evers H, Heidorn F, Gruber C. Investigative strategy for the forensic detection of sperm traces. Forensic Sci Med Pathol. 2009;5(3):182-188.
Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algoritm issledovaniya sledov krovi I vydelenij na veshchestvennyh dokazate’’stvah metodami kolichestvennogo immunofermentnogo analiza i kolorimetrii: metodicheskie rekomendacii. Moskva; 2021. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA, Cherpanova TV. Ustanovlenie nalichiya slyuny na veshchestvennyh dokazatel’stvah kolorimetricheskim metodom po α-amilaze: metodicheskie rekomendacii. Moskva; 2020. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel’stvah po kisloj fosfataze kolorimetricheskim metodom: metodicheskie rekomendacii. Moskva; 2012. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Cherepanova TV, Spiridonova OV, Yagmurov OD. Ustanovlenie nalichiya spermy na veshchestvennyh dokazatel’stvah po prostaticheskomu specificheskomu antigenu cheloveka s pomoshch’yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. Moskva; 2011. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Yagmurov OD. Ustanovlenie vidovoj prinadlezhnosti biologicheskih ob»ektov po IgG cheloveka s pomoshch’yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. Moskva; 2011. (In Russ.).
Laux DL. Development of biological standards for the quality assurance of presumptive testing reagents. Sci Justice. 2011;51(3):143-145.
Shuo J. Drainage fluid and scrum amylase levels accurately predict development of postoperative fistula. World J Gastroenterol. 2017;23(34):6357-6364.
Wandong H. Predictors of acute pancreatitis with low elevation of serum amylase. Ther Clin Risk Manag. 2017;13(1):1577-1584.
An J.H. Body fluid identification in forensics. BMB Rep. 2012;45(10):545-553.
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Comparative characteristics of methods for studying physical evidence used to establish the presence of blood and secretions in the Russian Federation and in foreign countries. Vestnik sudebnoy meditsiny. 2020;9(1):10-16. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Sidorov VL, Gusarov AA, Yagmurov OD. About the possibilities of high-technological methods for investigation of biological objects to establish the presence of sperm. Vestnik sudebnoy meditsiny. 2020;9(3):28-32. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. Determination the presence of saliva in traces on material evidence by α-amilase content using the colorimetry method. Vestnik sudebnoy meditsiny. 2020;9(3):17-22. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA. Ways of application of quantitative immuno-enzyme analysis for estimation of special belonging of biological objects and evidential detection of sperm on material evidence. Vestnik sudebnoy meditsiny. 2021;10(2):4-8. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. Application of quantitative methods for studying blood and body excretion tracks on material evidence in the performance of forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Portnova NA. The application of the colorimetric method for detecting sperm and saliva based on material evidence. Vestnik sudebnoy meditsiny. 2021;10(1):22-26. (In Russ.).