Determination of tissular and species membership of hyaline cartilage microparticles by forensic cytology and enzyme-linked immunosorbent assay methods

Gusarov A.A.; Sidorov V.L.; Surikova N.E.; Yagmurov O.D.

doi:https://doi.org/10.17116/sudmed20256802121

Gusarov A.A.

Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Expertise, Moscow Health Department

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medicine

ORCID: 0000-0002-8141-4488

Determination of tissular and species membership of hyaline cartilage microparticles by forensic cytology and enzyme-linked immunosorbent assay methods

Authors:

Gusarov A.A., Sidorov V.L., Surikova N.E., Yagmurov O.D.

More about the authors

Journal: Forensic Medical Expertise. 2025;68(2): 21‑24

DOI: 10.17116/sudmed20256802121

Read: 477 times

Contact the author

Table of contents

To cite this article:

Gusarov AA, Sidorov VL, Surikova NE, Yagmurov OD. Determination of tissular and species membership of hyaline cartilage microparticles by forensic cytology and enzyme-linked immunosorbent assay methods. Forensic Medical Expertise. 2025;68(2):21‑24. (In Russ.)
https://doi.org/10.17116/sudmed20256802121

Подписка 2026 Судебно-медицинская экспертиза (Forensic Medical Expertise)

Использование инфографики авторами научных работ

Recommended articles:

Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2024;(6):21-24

Methodology for performing intralaboratory quality control in establishing species of the blood by IgGtotal with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025;(1):38-42

Organ and species identification of liver microparticles using forensic cytology and quantitative enzyme-linked immunosorbent assay methods. Forensic Medical Expertise. 2025;(4):29-33

Abstract:

The article presents the results of hyaline cartilage microparticles study by forensic cytology and quantitative enzyme-linked immunosorbent assay. Hyaline cartilage cells’ polymorphism was demonstrated as a result of the performed forensic cytological study. The high sensitivity of method was confirmed, the indicators of optic density and total IgG concentration were determined, as well as the optimal values of minimal and maximal calibration samples were identified using enzyme-linked immunosorbent assay. The obtained data can be used by experts of forensic units when determining tissular and species membership of hyaline cartilage microparticles by forensic cytology and enzyme-linked immunosorbent assay methods.

Keywords:

hyaline cartilage microparticles

quantitative enzyme-linked immunosorbent assay

species membership

Authors:

Gusarov A.A.

Russian Centre of Forensic Medical Expertise

ORCID: 0000-0003-4270-1609

Sidorov V.L.

Bureau of Forensic Medical Examination

ORCID: 0000-0002-2214-895X

Surikova N.E.

Bureau of Forensic Medical Expertise, Moscow Health Department

ORCID: 0009-0005-0314-9569

Yagmurov O.D.

Bureau of Forensic Medicine

ORCID: 0000-0002-8141-4488

Received:

12.03.2024

Accepted:

15.03.2024

Close metadata

References:

Gusarov AA, Surikova NE. Metodika proizvodstva sudebno-citologicheskih issledovanij po delam o polovyh prestuplenijah: uchebnoe posobie. Moskva: Rossijskiy centr sudebno-medicinskoj ekspertizy; 2023. (In Russ.).
Sidorov VL, Gusarov AA. On the use of the enzyme immunoassay method in foreign forensic practice. Meditsinskaya ekspertiza i pravo. 2012;1:4-7. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Comparative characteristics of methods for studying physical evidence used to establish the presence of blood and secretions in the Russian Federation and in foreign countries. Vestnik sudebnoy meditsiny. 2020;9(1):10-16. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Isakova IV, Khorovskaya LA, Portnova NA. Algoritm issledovaniya sledov krovi i vydeleniy na veshchestvennyh dokazatel’stvah metodami kolichestvennogo immunofermentnogo analiza i kolorimetrii. Metodicheskie rekomendatsii. Moskva; 2021. (In Russ.).
Sidorov VL, Gusarov AA, Isakova IV, Yagmurov OD. Ustanovlenie vidovoj prinadlezhnosti biologicheskih ob»ektov po IgG cheloveka s pomoshch’yu kolichestvennogo tverdofaznogo immunofermentnogo analiza: usovershenstvovannaya medicinskaya tekhnologiya. Moskva; 2011. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA. Establishment of the species identity of bone fragments by IgGtotal using quantitative enzyme-linked immunosorbent assay. Vestnik sudebnoy meditsiny. 2020;12(3):14-18. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA. Ways of application of quantitative immuno-enzyme analysis for estimation of special belonging of biological objects and evidential detection of sperm on material evidence. Vestnik sudebnoy meditsiny. 2021;10(2):4-8. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Khorovskaya LA. Application of quantitative methods for studying traces of blood and secretions on material evidence during forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Gusarov AA, Sidorov VL, Yagmurov OD, Nagornov MN. Methodology for performing intralaboratory quality control in establishing species of the blood by IgG total with the use of quantitative enzyme-linked immunosorbent assay. Forensic Medical Expertise. 2025; 68(1): 38-42. (In Russ.). https://doi.org/10.17116/sudmed20256801138
Sidorov VL, Gusarov AA, Yagmurov OD. About the possibilities of high-technological methods for investigation of biological objects to establish the presence of sperm. Vestnik sudebnoy meditsiny. 2020;9(3):28-32. (In Russ.)
Gusarov A.A., Sidorov V.L., Yagmurov O.D., Surikova N.E. Establishing the species belonging of fragments and particles of striated muscle tissue by total IgG using quantitative solid-phase enzyme-linked immunosorbent assay. Sudebno-meditsinskaya ekspertiza. https://doi.org/10.17116/sudmed20246706121
Sidorov VL, Gusarov AA, Surikova NE, Khorovskaya LA, Loban IE (2019). Possibilities of assessing intralaboratory quality control when determining the concentration of PSAtotal in water extracts from semen stains on material. Vestnik sudebnoy meditsiny. 2019;8(3):20-27.
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA (2020). Determination the presence of saliva in traces on material evidence by α-amilase content using the colorimetry method. Vestnik sudebnoy meditsiny. 2020;9(3):17-22. (In Russ.).
Sidorov VL, Gusarov AA, Surikova NE, Khorovskaya L.A. Study of the stability of PSA samples in aqueous extracts used to establish the presence of sperm on physical evidence. Vestnik sudebnoy meditsiny. 2019;8(3):4-9.
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. (2020). Application of quantitative methods for studying blood and body excretion tracks on material evidence in the performance of forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Portnova NA. The application of the colorimetric method for detecting sperm and saliva based on material evidence. Vestnik sudebnoy meditsiny. 2021;10(1):22-26. (In Russ.).
Sidorov VL, Loban IE, Gusarov AA, Portnova NA, Horovskaya LA. Application of quantitative methods for studying blood and body excretion tracks on material evidence in the performance of forensic biological examinations. Vestnik sudebnoy meditsiny. 2020;9(2):28-34. (In Russ.).
Sidorov VL, Yagmurov OD, Gusarov AA, Portnova NA. The application of the colorimetric method for detecting sperm and saliva based on material evidence. Vestnik sudebnoy meditsiny. 2021;10(1):22-26. (In Russ.).