The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-0604
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Petrukhina N.B.

Kafedra stomatologii FPPOV GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova" Minzdrava RF

Zorina O.A.

GBOU VPO "Pervyĭ MGMU imeni I.M. Sechenova"

Shikh E.V.

Kafedra klinicheskoĭ farmakologii i propedevtiki vnutrennikh bolezneĭ GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova"

Shibaeva A.V.

Institut biokhimicheskoĭ fiziki im. N.M. Émanuélia RAN, Moskva;
Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Shevelev A.B.

Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Study of mutual dependence of periodontal and colonic microbiome in health and pathology using NSG-sequencing

Authors:

Petrukhina N.B., Zorina O.A., Shikh E.V., Shibaeva A.V., Shevelev A.B.

More about the authors

Journal: Stomatology. 2016;95(2): 8‑13

DOI: 10.17116/stomat20169528-13

Views: 892

Downloaded: 10

Download PDF (RU)
Contact the author
Table of contents

STUDY OF MUTUAL DEPENDENCE OF PERIODONTAL AND COLONIC MICROBIOME IN HEALTH AND PATHOLOGY USING NSG-SEQUENCING
STUDY OF MUTUAL DEPENDENCE OF PERIODONTAL AND COLONIC MICROBIOME IN HEALTH AND PATHOLOGY USING NSG-SEQUENCING

To cite this article:

Petrukhina NB, Zorina OA, Shikh EV, Shibaeva AV, Shevelev AB. Study of mutual dependence of periodontal and colonic microbiome in health and pathology using NSG-sequencing. Stomatology. 2016;95(2):8‑13. (In Russ.)
https://doi.org/10.17116/stomat20169528-13

Подписка 2025-2 (Stomatology)
 
МСФ на ЯМ
Close metadata
Recommended articles:
Prevention of periodontal inflammation in microgravity mode­ling. Stomatology. 2024;(6-2):23-28
Evolutionary trends in the evolution of the microelemental composition of enamel, surface layers of dentin, cement of human teeth in the North-Western region of Sibe­ria in the X—XX centuries. Stomatology. 2025;(1):5-8
Vita­min D role in oral cavity diseases. Stomatology. 2025;(1):81-86
Functional occlusal contacts of teeth in patients with periodontitis. Russian Journal of Stomatology. 2025;(1):11-17
Modern scientific and practical approaches to searching for biomarkers of aging. Rege­nerative Biotechnologies, Preventive, Digi­tal and Predictive Medi­cine. 2024;(3):46-52
Age-related features of the gut microbiota composition of Rhesus monkeys kept in captivity. Mole­cular Gene­tics, Microbiology and Viro­logy. 2024;(3):22-28
Specifics of cancer dete­ction at the outpatient stage. A case report. Russian Journal of Preventive Medi­cine. 2024;(10):97-100
Inte­gration of NGS testing with conventional mole­cular gene­tic methods in onco­logy. P.A. Herzen Journal of Onco­logy. 2024;(6):84-90
High-throughput sequencing: a powe­rful tool for pathogen dete­ction and identification in clinical samples. Labo­ratory Service. 2024;(4):49-56
Aica­rdi—Goutieres syndrome type 6 asso­ciated with a compound hete­rozygous variant in ADAR: a first case report in the Russian popu­lation. S.S. Korsakov Journal of Neurology and Psychiatry. 2025;(1):131-138

By using NGS-sequencing libraries of DNA from periodontal swabs with primers specific to V6 region of 16S rDNA prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. Hyper-colonization of the colon with Akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10–6). This result is in a good agreement with communications about positive impact of hyper-colonization of the colon with this species on type 2 diabetes, obesity, atopic dermatitis, and antibiotic-induced diarrhea associated with Clostridium dificile. Analysis of the periodontal protectors at the periodontium elucidated a number of close taxonomic relatives of the periodontal pathogens by Socransky, e.g. Aggregatibacter segnis and Aggregatibacter aphrophilus are closely related to Aggregatibacter actinomycetemcomitans; Treponema vencentii is a relative of Treponema denticola; Prevotella baroniae, Prevotella salivae and Prevotella spp. are relatives of Prevotella intermedia; Campylobacter concisus is a relative of Campylobacter jejuni, causative agent of enterocolitis.

Keywords:

periodontium
periodontitis
colon
periodontal pathogen
genom
16S rRNA
16S rDNA
Illumina
sequencing
NGS
Akkermansia muciniphila

Authors:

Petrukhina N.B.

Kafedra stomatologii FPPOV GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova" Minzdrava RF

Zorina O.A.

GBOU VPO "Pervyĭ MGMU imeni I.M. Sechenova"

Shikh E.V.

Kafedra klinicheskoĭ farmakologii i propedevtiki vnutrennikh bolezneĭ GBOU VPO "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova"

Shibaeva A.V.

Institut biokhimicheskoĭ fiziki im. N.M. Émanuélia RAN, Moskva;
Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

Shevelev A.B.

Institut poliomielita i virusnykh éntsefalitov im. M.P. Chumakova RAMN, Moskva

List of references:

  1. Zorina O.A., Kulakov A.A., Boriskina O.A. Metod PTsR «v real'nom vremeni» dlya analiza kolichestvennogo i kachestvennogo sootnoshenii mikrobiotsenoza parodontal'nogo karmana. Stomatologiya. 2011;3:31-33.
  2. Adler I, Muiño A, Aguas S, Harada L, Diaz M, Lence A, Labbrozzi M, Muiño JM, Elsner B, Avagnina A, Denninghoff V. Helicobacter pylori and oral pathology: Relationship with the gastric infection. World J Gastroenterol. 2014;20(29):9922-9935. doi: 10.3748/wjg.v20.i29.9922.
  3. Belstrom D, Fiehn NE, Nielsen CH, Kirkby N, Twetman S et al. Differences in bacterial saliva profile between periodontitis patients and a control cohort. Journal of clinical periodontology. 2014;41:104-112. doi: 10.1111/jcpe.12190.
  4. Bradlow HL. Obesity and the gut microbiome: pathophysiological aspects. Horm Mol Biol Clin Investig. 2014;17(1):53-61. doi: 10.1515/hmbci-2013-0063.
  5. Everard A. Cross-talk between Akkermansia muciniphila and intestinal epithelium controls diet-induced obesity. Proc Natl Acad Sci USA. 2013;110(22):9066-9071. doi: 10.1073/pnas.1219451110.
  6. Halmos EP, Christophersen CT, Bird AR, Shepherd SJ, Gibson PR, Muir JG. Diets that differ in their FODMAP content alter the colonic luminal microenvironment. Gut. 2015;64(1):93-100. doi: 10.1136/gutjnl-2014-307264.
  7. Hansen AK, Hansen CH, Krych L, Nielsen DS. Impact of the gut microbiota on rodent models of human disease. World J Gastroenterol. 2014;20(47):17727-17736. doi: 10.3748/wjg.v20.i47.17727.
  8. Huse SM, Dethlefsen L, Huber JA, Mark Welch D, Relman DA. Exploring microbial diversity and taxonomy using SSU rRNA hypervariable tag sequencing. PLoS genetics. 2008;4:1000255. doi: 10.1371/journal.pgen.1000255.
  9. Junemann S, Prior K, Szczepanowski R, Harks I, Ehmke B et al. Bacterial community shift in treated periodontitis patients revealed by ion torrent 16S rRNA gene amplicon sequencing. PloS one. 2012;7:41606. doi: 10.1371/journal.pone.0041606.
  10. Keijser BJ, Zaura E, Huse SM, van der Vossen JM, Schuren FH et al. Pyrosequencing analysis of the oral microflora of healthy adults. Journal of dental research. 2008;87:1016-1020. doi: 10.1177/154405910808701104.
  11. Lazarevic V, Whiteson K, Huse S, Hernandez D, Farinelli L et al. Metagenomic study of the oral microbiota by Illumina high-throughput sequencing. Journal of microbiological methods. 2009;79:266-271. doi: 10.1016/j.mimet.2009.09.012.
  12. Loesche WJ, Grossman NS. Periodontal disease as a specific, albeit chronic, infection: diagnosis and treatment. Clin Microbiol Rev. 2001;14(10):727-752. doi: 10.1128/CMR.14.4.727-752.2001.
  13. Nabwera HM, Logan RP. Epidemiology of Helicobacter pylori: transmission, translocation and extragastric reservoirs. J Physiol Pharmacol. 1999;50:711-722.
  14. Wroblewski LE, Peek RM, Wilson KT. Helicobacter pylori and gastric cancer: factors that modulate disease risk. Clin Microbiol Rev. 2010;23:713-739. doi: 10.1128/CMR.00011-10.

Close metadata

Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.