Cellular technologies in gynecology and reproductive medicine. (Literature review and own data)

Adamyan L.V.; Pivazyan L.G.; Kurbatova K.S.; Mailova K.S.; Osipova A.A.; Stepanian A.A.

doi:https://doi.org/10.17116/repro20253105130

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Abstract:

Cellular technologies represent one of the most promising directions in gynecology and reproductive medicine, offering fundamentally novel approaches to the treatment of infertility and other reproductive function disorders. Unlike conventional therapeutic modalities, cell-based therapy targets the pathogenetic mechanisms of disease, promoting restoration of the structural and functional integrity of reproductive organs at the tissue and cellular levels. This article presents an analysis of contemporary cellular technologies employed in gynecology and reproductive medicine, including mesenchymal stem cells of various origins (bone marrow, adipose tissue, umbilical cord, placenta, menstrual blood, and endometrium), extracellular vesicles (exosomes and apoptotic bodies), platelet-rich plasma, microRNA therapy, tissue-engineered scaffolds, bioprinting, and organoid technologies. Particular emphasis is placed on the mechanisms of therapeutic action, encompassing immunomodulation, angiogenesis stimulation, fibrosis suppression, and paracrine secretion of bioactive factors. The results of clinical and experimental studies demonstrating the efficacy of cellular technologies in Asherman’s syndrome, thin endometrium, chronic endometritis, and premature ovarian insufficiency are examined in detail. Evidence indicates that mesenchymal stem cells are capable of restoring ovarian function, stimulating endometrial regeneration, enhancing implantation potential, and increasing pregnancy rates. Contemporary approaches, including bioengineering of extracellular vesicles, utilization of biocompatible scaffolds, and three-dimensional bioprinting, unveil novel opportunities for personalized regenerative therapy. The article also addresses existing limitations and future prospects for the development of cellular technologies, including the necessity for protocol standardization, implementation of large-scale controlled trials, and development of objective predictors of therapeutic response. Cellular technologies constitute a promising strategy for creating innovative, pathogenetically justified treatment methods aimed at restoring reproductive function and improving the efficacy of infertility management.

Keywords:

cellular technologies

mesenchymal stem cells

exosomes

bioengineering

reproductive medicine

infertility

Authors:

Adamyan L.V.

National medical research center for obstetrics, gynecology and perinatology named after academician V.I. Kulakov;
Russian university of medicine

SPIN RSCI: 9836-2713
Scopus AuthorID: 7006372422
ResearcherID: Q-1722-2018
ORCID: 0000-0002-3253-4512

Pivazyan L.G.

National medical research center for obstetrics, gynecology and perinatology named after academician V.I. Kulakov

ORCID: 0000-0002-6844-3321

Kurbatova K.S.

Department of Obstetrics, Gynecology and Reproductive Medicine, Russian University of Medicine

ORCID: 0009-0008-8568-630X

Mailova K.S.

FSBEI HE «RosUniMed» of MOH of Russia

ORCID: 0000-0003-2723-3114

Osipova A.A.

Russian Medical University

Stepanian A.A.

Academia of Women’s Health and Endoscopic Surgery

Received:

07.10.2025

Accepted:

22.10.2025

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References:

Margiana R, Pallathadka H, Mansouri S, Qasim QA, Kumar A, Kumar A. Strategies in Tissue Regenerative Engineering for the Treatment of Human Infertility. Stem Cell Reviews and Reports. 2025; 21:2548-2564. https://doi.org/10.1007/s12015-025-10964-y
Patent RF №RU 2748246 C1. Opubl. 21.05.2021. Adamyan LV, Smolnikova VYu, Asaturova AV, Dementyeva VO. Odnoetapnyj khirurgicheskij metod aktivatsii funktsii yaichnikov dlya lecheniya prezhdevremennoj nedostatochnosti yaichnikov i vosstanovleniya ovarial’noj funktsi. (In Russ.).
Rodríguez-Eguren A, Bueno-Fernandez C, Gómez-Álvarez M, Francés-Herrero E, Pellicer A, Bellver J. Evolution of biotechnological advances and regenerative therapies for endometrial disorders: a systematic review. Human Reproduction Update. 2024;30: 584-613. https://doi.org/10.1093/humupd/dmae013
Patent USSR №1191087 A1. Opubl. 15.11.1985. Adamyan LV, Pobedinskiy NM, Selezneva ND. Sposob lecheniya vtorichnoy amenorei. (In Russ.).
Kulakov VI, Adamyan LV, My`nbaev OA. Posleoperacionny`e spajki (e`tiologiya, patogenez i profilaktika). M.: Medicina; 1998. (In Russ.).
Kulakov VI, Adamyan LV, Askolskaya SI. Gisterektomiya i zdorovye zhenshchiny. M.: Meditsina; 1999. (In Russ.).
Mailova KS. Faktory` riska i profilaktika spaechnogo processa pri laparoskopii v ginekologii: Dis. … kand. med. nauk. M., 2012. (In Russ.).
Kavaldzhieva K, Mladenov N, Markova M, Belemezova K. Mesenchymal Stem Cell Secretome: Potential Applications in Human Infertility Caused by Hormonal Imbalance, External Damage, or Immune Factors. Biomedicines. 2025;13:586. https://doi.org/10.3390/biomedicines13030586
Sittadjody S, Criswell T, Jackson JD, Atala A, Yoo JJ. Regenerative Medicine Approaches in Bioengineering Female Reproductive Tissues. Reproductive sciences. 2021;28:1573-1595. https://doi.org/10.1007/s43032-021-00548-9
Wu J-X, Xia T, She L-P, Lin S, Luo X-M. Stem Cell Therapies for Human Infertility: Advantages and Challenges. Cell Transplantation. 2022;31:9636897221083252. https://doi.org/10.1177/09636897221083252
Hou C, Zhu H, Chang X. Mesenchymal stem cells: opening a new chapter in the treatment of gynecological diseases. Stem Cell Research and Therapy. 2025;16:520. https://doi.org/10.1186/s13287-025-04623-9
Lee D, Kang Y-J, Song H. Regenerating the uterus: translational advances in endometrial bioengineering and immunotherapeutics. Seminars in Immunopathology. 2025;47:36. https://doi.org/10.1007/s00281-025-01063-8
Babaei K, Aziminezhad M, Norollahi SE, Vahidi S, Samadani AA. Cell therapy for the treatment of reproductive diseases and infertility: an overview from the mechanism to the clinic alongside diagnostic methods. Frontiers of Medicine. 2022;16:827-858. https://doi.org/10.1007/s11684-022-0948-8
Li Y, Chen F, Zhao W, Sun D, Zhang L, Qiao R. Harnessing extracellular vesicles from adipose-derived stem cells for the treatment of 4-vinylcyclohexene diepoxide-induced premature ovarian insufficiency. Stem Cell Research and Therapy. 2025;16:425. https://doi.org/10.1186/s13287-025-04553-6
Cantero MJ, Bueloni B, Gonzalez Llamazares L, Fiore E, Lameroli L, Atorrasagasti C. Modified mesenchymal stromal cells by in vitro transcribed mRNA: a therapeutic strategy for hepatocellular carcinoma. Stem Cell Research and Therapy. 2024;15:208. https://doi.org/10.1186/s13287-024-03806-0
Adamyan LV, Pivazyan LG. Interdisciplinary approach and the cur rent state of the issue of premature ovarian aging (literature review). Russian Journal of Human Reproduction. 2023;29(1):94-103. (In Russ.). https://doi.org/10.17116/repro20232901194
Lou S, Duan Y, Nie H, Cui X, Du J, Yao Y. Mesenchymal stem cells: Biological characteristics and application in disease therapy. Biochimie. 2021;185:9-21. https://doi.org/10.1016/j.biochi.2021.03.003
Wang Y, Fang J, Liu B, Shao C, Shi Y. Reciprocal regulation of mesenchymal stem cells and immune responses. Cell Stem Cell. 2022; 29:1515-1530. https://doi.org/10.1016/j.stem.2022.10.001
Yadav S, Maity P, Kapat K. The Opportunities and Challenges of Mesenchymal Stem Cells-Derived Exosomes in Theranostics and Regenerative Medicine. Cells. 2024;13:1956. https://doi.org/10.3390/cells13231956
Erceg Ivkošić I, Fureš R, Ćosić V, Mikelin N, Bulić L, Dobranić D. Unlocking the Potential of Mesenchymal Stem Cells in Gynecology: Where Are We Now? Journal of Personalized Medicine. 2023; 13:1253. https://doi.org/10.3390/jpm13081253
Nair R, Agarwal P, Gadre MA, Vasanthan KS, Seetharam RN. Stem cell treatments for female reproductive disorders: a comprehensive review. Journal of Ovarian Research. 2025;18:161. https://doi.org/10.1186/s13048-025-01750-y
Allouh MZ, Rizvi SFA, Alamri A, Jimoh Y, Aouda S, Ouda ZH. Mesenchymal stromal/stem cells from perinatal sources: biological facts, molecular biomarkers, and therapeutic promises. Stem Cell Research and Therapy. 2025;16:127. https://doi.org/10.1186/s13287-025-04254-0
Adamyan LV, Sibirskaya EV, Pivazyan LG, Dzharullayeva DS, Avetisyan DS, Zakaryan AA. Allotransplantation and autotransplantation in obstetrics and gynecology. E`ffektivnaya farmakoterapiya. (In Russ.). https://doi.org/10.33978/2307-3586-2025-21-20-70-75
Zhidu S, Ying T, Rui J, Chao Z. Translational potential of mesenchymal stem cells in regenerative therapies for human diseases: challenges and opportunities. Stem Cell Research and Therapy. 2024; 15:266. https://doi.org/10.1186/s13287-024-03885-z
Chu D-T, Phuong TNT, Tien NLB, Tran DK, Thanh VV, Quang TL. An Update on the Progress of Isolation, Culture, Storage, and Clinical Application of Human Bone Marrow Mesenchymal Stem/Stromal Cells. International Journal of Molecular Sciences. 2020; 21:708. https://doi.org/10.3390/ijms21030708
Han X, Liao R, Li X, Zhang C, Huo S, Qin L. Mesenchymal stem cells in treating human diseases: molecular mechanisms and clinical studies. Signal Transduction and Targeted Therapy. 2025;10:262. https://doi.org/10.1038/s41392-025-02313-9
Yuan L, Cao J, Hu M, Xu D, Li Y, Zhao S. Bone marrow mesenchymal stem cells combined with estrogen synergistically promote endometrial regeneration and reverse EMT via Wnt/β-catenin signaling pathway. Reproductive Biology and Endocrinology. 2022;20:121. https://doi.org/10.1186/s12958-022-00988-1
Xiong Z, Ma Y, He J, Li Q, Liu L, Yang C. Apoptotic bodies of bone marrow mesenchymal stem cells inhibit endometrial stromal cell fibrosis by mediating the Wnt/β-catenin signaling pathway. Heliyon. 2023;9:e20716. https://doi.org/10.1016/j.heliyon.2023.e20716
Wang Z, Xia L, Cheng J, Liu J, Zhu Q, Cui C. Combination Therapy of Bone Marrow Mesenchymal Stem Cell Transplantation and Electroacupuncture for the Repair of Intrauterine Adhesions in Rats: Mechanisms and Functional Recovery. Reproductive Sciences. 2024;31(23):18-30. https://doi.org/10.1007/s43032-024-01465-3
Hua Z, Wei P. Human Stromal Cell Aggregates Concentrate Adipose Tissue Constitutive Cell Population by In Vitro DNA Quantification Analysis. Plastic and Reconstructive Surgery. 2021;148:1061e. https://doi.org/10.1097/PRS.0000000000008548
Lau CS, Park SY, Ethiraj LP, Singh P, Raj G, Quek J. Role of Adipose-Derived Mesenchymal Stem Cells in Bone Regeneration. International Journal of Molecular Sciences. 2024;25:6805. https://doi.org/10.3390/ijms25126805
Çil N, Yaka M, Ünal MS, Dodurga Y, Tan S, Seçme M. Adipose derived mesenchymal stem cell treatment in experimental asherman syndrome induced rats. Molecular Biology Reports. 2020;47:4541-4552. https://doi.org/10.1007/s11033-020-05505-4
Dai Y, Xin L, Hu S, Xu S, Huang D, Jin X. A construct of adipose-derived mesenchymal stem cells-laden collagen scaffold for fertility restoration by inhibiting fibrosis in a rat model of endometrial injury. Regenerative Biomaterials. 2023;10:rbad080. https://doi.org/10.1093/rb/rbad080
Adamyan LV, Malyshev IY, Pivazyan LG, Yurkanova MD, Mailova KS, Stepanian AA. The role of macrophage polarization in the development of endometriosis and the therapeutic potential of its modulation. Russian Journal of Human Reproduction. 2025;31(3): 20-33. (In Russ.). https://doi.org/10.17116/repro20253103120
Benny M, Courchia B, Shrager S, Sharma M, Chen P, Duara J. Comparative Effects of Bone Marrow-derived Versus Umbilical Cord Tissue Mesenchymal Stem Cells in an Experimental Model of Bronchopulmonary Dysplasia. Stem Cells Translational Medicine. 2022;11:189-199. https://doi.org/10.1093/stcltm/szab011
Hori A, Takahashi A, Miharu Y, Yamaguchi S, Sugita M, Mukai T. Superior migration ability of umbilical cord-derived mesenchymal stromal cells (MSCs) toward activated lymphocytes in comparison with those of bone marrow and adipose-derived MSCs. Frontiers in Cell and Developmental Biology. 2024;12:1329218. https://doi.org/10.3389/fcell.2024.1329218
Timoneri F, Lo Iacono M, Corrao S, Alberti G, Amico G, Corsello T. Wharton’s jelly mesenchymal stromal cells derived from preterm umbilical cord reveal a hepatogenic potential. Frontiers in Cell and Developmental Biology. 2025;13:1626353. https://doi.org/10.3389/fcell.2025.1626353
Yin N, Wu C, Qiu J, Zhang Y, Bo L, Xu Y. Protective properties of heme oxygenase-1 expressed in umbilical cord mesenchymal stem cells help restore the ovarian function of premature ovarian failure mice through activating the JNK/Bcl-2 signal pathway-regulated autophagy and upregulating the circulating of CD8+CD28- T cells. Stem Cell Research and Therapy. 2020;11:49. https://doi.org/10.1186/s13287-019-1537-x
Zhang D, Du Q, Li C, Ding C, Chen J, He Y. Functionalized human umbilical cord mesenchymal stem cells and injectable HA/Gel hydrogel synergy in endometrial repair and fertility recovery. Acta Biomaterialia. 2023;167:205-218. https://doi.org/10.1016/j.actbio.2023.06.013
Liu Y, Cai J, Luo X, Wen H, Luo Y. Collagen Scaffold with Human Umbilical Cord Mesenchymal Stem Cells Remarkably Improves Intrauterine Adhesions in a Rat Model. Gynecologic and Obstetric Investigation. 2020;85:267-276. https://doi.org/10.1159/000505691
Kim M, Bae YK, Um S, Kwon JH, Kim G-H, Choi SJ. A Small-Sized Population of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Shows High Stemness Properties and Therapeutic Benefit. Stem Cells International. 2020;2020:1-17. https://doi.org/10.1155/2020/5924983
Shang Y, Guan H, Zhou F. Biological Characteristics of Umbilical Cord Mesenchymal Stem Cells and Its Therapeutic Potential for Hematological Disorders. Frontiers in Cell and Developmental Biology. 2021;9:570179. https://doi.org/10.3389/fcell.2021.570179
Mebarki M, Moine-Picard C, Enjaume-Rauch R, Laurent-Puig A, Suissa A, Feyants V. Pooling umbilical cord-mesenchymal stromal cells derived from selected multiple donors reduces donor-dependent variability and improves their immunomodulatory properties. Stem Cell Research and Therapy. 2025;16:252. https://doi.org/10.1186/s13287-025-04361-y
Wang JJ, Hua Q, Li HJ, Zhang DM, Wang YL. Effect of mesenchymal stem cell derived from umbilical cord blood on rabbit intrauterine adhesion model. Zhonghua Yi Xue Za Zhi. 2024;104:3757-3764. https://doi.org/10.3760/cma.j.cn112137-20240314-00578
Yi X, Chen F, Liu F, Peng Q, Li Y, Li S. Comparative separation methods and biological characteristics of human placental and umbilical cord mesenchymal stem cells in serum-free culture conditions. Stem Cell Research and Therapy. 2020;11:183 https://doi.org/10.1186/s13287-020-01690-y
Li H, Zhao W, Wang L, Luo Q, Yin N, Lu X. Human placenta-derived mesenchymal stem cells inhibit apoptosis of granulosa cells induced by IRE1α pathway in autoimmune POF mice. Cell Biology International. 2019;43:899-909. https://doi.org/10.1002/cbin.11165
Ghamari S-H, Abbasi-Kangevari M, Tayebi T, Bahrami S, Niknejad H. The Bottlenecks in Translating Placenta-Derived Amniotic Epithelial and Mesenchymal Stromal Cells into the Clinic: Current Discrepancies in Marker Reports. Frontiers in Bioengineering and Biotechnology. 2020;8:180. https://doi.org/10.3389/fbioe.2020.00180
Vaiciuleviciute R, Pachaleva J, Bernotiene E, Kugaudaite G, Lebedis I, Krugly E. Menstrual blood-derived mesenchymal stromal cell extracellular vesicles — a potential tool for tissue regeneration and disease detection. Frontiers in Bioengineering and Biotechnology. 2025;13:1643408. https://doi.org/10.3389/fbioe.2025.1643408
Sanchez-Mata A, Gonzalez-Muñoz E. Understanding menstrual blood-derived stromal/stem cells: Definition and properties. Are we rushing into their therapeutic applications? iScience. 2021;24:103501. https://doi.org/10.1016/j.isci.2021.103501
Hu X, Dai Z, Pan R, Zhang Y, Liu L, Wang Y. Long-term transplantation human menstrual blood mesenchymal stem cell loaded collagen scaffolds repair endometrium histological injury. Reproductive Toxicology. 2022;109:53-60. https://doi.org/10.1016/j.reprotox.2022.03.001
Chen X, Wu Y, Wang Y, Chen L, Zheng W, Zhou S. Human menstrual blood-derived stem cells mitigate bleomycin-induced pulmonary fibrosis through anti-apoptosis and anti-inflammatory effects. Stem Cell Research and Therapy. 2020;11:477. https://doi.org/10.1186/s13287-020-01926-x
Bozorgmehr M, Gurung S, Darzi S, Nikoo S, Kazemnejad S, Zarnani A-H. Endometrial and Menstrual Blood Mesenchymal Stem/Stromal Cells: Biological Properties and Clinical Application. Frontiers in Cell and Developmental Biology. 2020;8:497. https://doi.org/10.3389/fcell.2020.00497
Cousins FL, Filby CE, Gargett CE. Endometrial Stem/Progenitor Cells–Their Role in Endometrial Repair and Regeneration. Frontiers in Reproductive Health. 2022;3:811537. https://doi.org/10.3389/frph.2021.811537
He L, Li Q. Application of biomaterials in mesenchymal stem cell based endometrial reconstruction: current status and challenges. Frontiers in Bioengineering and Biotechnology. 2025;13:1518398. https://doi.org/10.3389/fbioe.2025.1518398
Wang X, Bao H, Liu X, Wang C, Hao C. Effects of endometrial stem cell transplantation combined with estrogen in the repair of endometrial injury. Oncology Letters. 2018;16:1115-1122. https://doi.org/10.3892/ol.2018.8702
Chatzianagnosti S, Dermitzakis I, Theotokis P, Kousta E, Mastorakos G, Manthou ME. Application of Mesenchymal Stem Cells in Female Infertility Treatment: Protocols and Preliminary Results. Life. 2024;14:1161. https://doi.org/10.3390/life14091161
Adamyan LV, Kuznetsova MV, Pivazyan LG, Davydova YuD, Trofimov DYu. Genetic aspects of endometriosis and adenomyosis: a modern view on the problem. Russian Journal of Human Reproduction. 2023;29(4-2):14-22. (In Russ.). https://doi.org/10.17116/repro20232904214
Adamyan LV, Avetisyan DS, Kuznetsova MV, Trofimov DYu, Pivazyan LG, Mailova KS, Osipova AA. The role of DNA methylation, histone modifications, and non-coding RNA expression in the pathogenesis of premature ovarian insufficiency and innovative ways to overcome infertility from the point of epigenetic disturbances. Russian Journal of Human Reproduction. 2025;31(3):6-19. (In Russ.). https://doi.org/10.17116/repro2025310316
Adamyan LV, Pivazyan LG, Antonova AA. Autoimmunity and premature ovarian insufficiency — up-to-date. Russian Journal of Human Reproduction. 2022;28(6):116-124. (In Russ.). https://doi.org/10.17116/repro202228061116
Kalluri R, LeBleu VS. The biology, function, and biomedical applications of exosomes. Science. 2020;367:eaau6977. https://doi.org/10.1126/science.aau6977
Liu M, Wen Z, Zhang T, Zhang L, Liu X, Wang M. The role of exosomal molecular cargo in exosome biogenesis and disease diagnosis. Frontiers in Immunology. 2024;15:1417758. https://doi.org/10.3389/fimmu.2024.1417758
Yao Y, Chen R, Wang G, Zhang Y, Liu F. Exosomes derived from mesenchymal stem cells reverse EMT via TGF-β1/Smad pathway and promote repair of damaged endometrium. Stem Cell Research and Therapy. 2019;10:225. https://doi.org/10.1186/s13287-019-1332-8
Zhu Q, Tang S, Zhu Y, Chen D, Huang J, Lin J. Exosomes Derived From CTF1-Modified Bone Marrow Stem Cells Promote Endometrial Regeneration and Restore Fertility. Frontiers in Bioengineering and Biotechnology. 2022;10:868734. https://doi.org/10.3389/fbioe.2022.868734
Li J, Pan Y, Yang J, Wang J, Jiang Q, Dou H. Tumor necrosis factor-α-primed mesenchymal stem cell-derived exosomes promote M2 macrophage polarization via Galectin-1 and modify intrauterine adhesion on a novel murine model. Frontiers in Immunology. 2022;13: 945234. https://doi.org/10.3389/fimmu.2022.945234
Liang L, Liu H, Wang S. Placental mesenchymal stem cell–derived exosomes treat endometrial injury in a rat model of intrauterine adhesions. Molecular Genetics and Genomics. 2025;300:36. https://doi.org/10.1007/s00438-025-02241-x
Jin X, Dai Y, Xin L, Ye Z, Chen J, He Q. ADSC-derived exosomes-coupled decellularized matrix for endometrial regeneration and fertility restoration. Materials Today Bio. 2023;23:100857. https://doi.org/10.1016/j.mtbio.2023.100857
Sun H, Dong J, Fu Z, Lu X, Chen X, Lei H. TSG6-Exo@CS/GP Attenuates Endometrium Fibrosis by Inhibiting Macrophage Activation in a Murine IUA Model. Advanced Materials. 2024;36: e2308921. https://doi.org/10.1002/adma.202308921
Battistelli M, Falcieri E. Apoptotic Bodies: Particular Extracellular Vesicles Involved in Intercellular Communication. Biology. 2020;9:21. https://doi.org/10.3390/biology9010021
Yu L, Zhu G, Zhang Z, Yu Y, Zeng L, Xu Z. Apoptotic bodies: bioactive treasure left behind by the dying cells with robust diagnostic and therapeutic application potentials. Journal of Nanobiotechnology. 2023;21:218. https://doi.org/10.1186/s12951-023-01969-1
Hoseinzadeh A, Esmaeili S-A, Sahebi R, Melak AM, Mahmoudi M, Hasannia M. Fate and long-lasting therapeutic effects of mesenchymal stromal/stem-like cells: mechanistic insights. Stem Cell Research and Therapy. 2025;16:33. https://doi.org/10.1186/s13287-025-04158-z
Tang H, Luo H, Zhang Z, Yang D. Mesenchymal Stem Cell-Derived Apoptotic Bodies: Biological Functions and Therapeutic Potential. Cells. 2022;11:3879. https://doi.org/10.3390/cells11233879
Critchley HOD, Maybin JA, Armstrong GM, Williams ARW. Physiology of the Endometrium and Regulation of Menstruation. Physiological Reviews. 2020;100:1149-1179. https://doi.org/10.1152/physrev.00031.2019
Xin L, Wei C, Tong X, Dai Y, Huang D, Chen J. In situ delivery of apoptotic bodies derived from mesenchymal stem cells via a hyaluronic acid hydrogel: A therapy for intrauterine adhesions. Bioactive Materials. 2022;12:107-119. https://doi.org/10.1016/j.bioactmat.2021.10.025
Liao X, Liang J-X, Li S-H, Huang S, Yan J-X, Xiao L-L. Allogeneic Platelet-Rich Plasma Therapy as an Effective and Safe Adjuvant Method for Chronic Wounds. Journal of Surgical Research. 2020;246:284-291. https://doi.org/10.1016/j.jss.2019.09.019
Adamyan LV, Antonova AA, Pivazyan LG, Krylova EI. The role of the use of platelet-rich plasma in reproductive medicine. Russian Journal of Human Reproduction. 2023;29(3):25-30. (In Russ.). https://doi.org/10.17116/repro20232903125
Huang S, Li Q, Li X, Ye H, Zhang L, Zhu X. Recent Research Progress of Wound Healing Biomaterials Containing Platelet-Rich Plasma. International Journal of Nanomedicine. 2025;20:3961-3976. https://doi.org/10.2147/IJN.S506677
Gentile P, Garcovich S. Systematic Review — the Potential Implications of Different Platelet-Rich Plasma (PRP) Concentrations in Regenerative Medicine for Tissue Repair. International Journal of Molecular Sciences. 2020;21:5702. https://doi.org/10.3390/ijms21165702
Chang Y, Li J, Chen Y, Wei L, Yang X, Shi Y. Autologous platelet-rich plasma promotes endometrial growth and improves pregnancy outcome during in vitro fertilization. International Journal of Clinical and Experimental Medicine. 2015;8:1286-1290.
Frantz N, Ferreira M, Kulmann MI, Frantz G, Bos-Mikich A, Oliveira R. Platelet-Rich plasma as an effective alternative approach for improving endometrial receptivity — a clinical retrospective study. JBRA Assisted Reproduction. 2020;24:442-446. https://doi.org/10.5935/1518-0557.20200026
Chang Y, Peng J, Zhu Y, Sun P, Mai H, Guo Q. How platelet-rich plasma (PRP) intra-uterine injection improve endometrial receptivity of intrauterine adhesions in women: A time-series-based self-controlled study. Journal of Reproductive Immunology. 2023;156: 103796. https://doi.org/10.1016/j.jri.2023.103796
Aghajanova L, Sundaram V, Kao C-N, Letourneau JM, Manvelyan E, Cedars MI. Autologous platelet-rich plasma treatment for moderate-severe Asherman syndrome: the first experience. Journal of Assisted Reproduction and Genetics. 2021;38:2955-2963. https://doi.org/10.1007/s10815-021-02328-5
Lin Y, Dong S, Ye X, Liu J, Li J, Zhang Y. Synergistic regenerative therapy of thin endometrium by human placenta-derived mesenchymal stem cells encapsulated within hyaluronic acid hydrogels. Stem Cell Research and Therapy. 2022;13:66. https://doi.org/10.1186/s13287-022-02717-2
Li Y, Cheng B, Tian J. Platelet-rich plasma may accelerate diabetic wound healing by modulating epithelial/endothelial-mesenchymal transition through inhibiting reactive oxygen species-mediated oxidative stress. Frontiers in Bioengineering and Biotechnology. 2025;13:1623780. https://doi.org/10.3389/fbioe.2025.1623780
Russell SJ, Kwok YSS, Nguyen TT-TN, Librach C. Autologous platelet-rich plasma improves the endometrial thickness and live birth rate in patients with recurrent implantation failure and thin endometrium. Journal of Assisted Reproduction and Genetics. 2022;39: 1305-1312. https://doi.org/10.1007/s10815-022-02505-0
Arif KMT, Elliott EK, Haupt LM, Griffiths LR. Regulatory Mechanisms of Epigenetic miRNA Relationships in Human Cancer and Potential as Therapeutic Targets. Cancers. 2020;12:2922. https://doi.org/10.3390/cancers12102922
Panni S, Pizzolotto R. Integrated Analysis of microRNA Targets Reveals New Insights into Transcriptional-Post-Transcriptional Regulatory Cross-Talk. Biology. 2025;14:43. https://doi.org/10.3390/biology14010043
Sadakierska-Chudy A. MicroRNAs: Diverse Mechanisms of Action and Their Potential Applications as Cancer Epi-Therapeutics. Biomolecules. 2020;10:1285. https://doi.org/10.3390/biom10091285
Khan I, Siraj M. An updated review on cell signaling pathways regulated by candidate miRNAs in coronary artery disease. Non-Coding RNA Research. 2023;8:326-334. https://doi.org/10.1016/j.ncrna.2023.03.007
Voros C, Varthaliti A, Athanasiou D, Mavrogianni D, Bananis K, Athanasiou A. MicroRNA Signatures in Endometrial Receptivity — Unlocking Their Role in Embryo Implantation and IVF Success: A Systematic Review. Biomedicines. 2025;13:1189. https://doi.org/10.3390/biomedicines13051189
Shekibi M, Heng S, Nie G. MicroRNAs in the Regulation of Endometrial Receptivity for Embryo Implantation. International Journal of Molecular Sciences. 2022;23:6210. https://doi.org/10.3390/ijms23116210
Li J, Du S, Sheng X, Liu J, Cen B, Huang F. MicroRNA-29b Inhibits Endometrial Fibrosis by Regulating the Sp1-TGF-β1/Smad-CTGF Axis in a Rat Model. Reproductive Sciences. 2016;23:386-394. https://doi.org/10.1177/1933719115602768
Won JE, Park M, Hong S-H, Kim YS, Song H. Quantum dots as biocompatible small RNA nanocarriers modulating macrophage polarization to treat Asherman’s syndrome. NPJ Regenerative Medicine. 2025;10:15. https://doi.org/10.1038/s41536-025-00403-4
Mani A, Hotra JW, Blackwell SC, Goetzl L, Refuerzo JS. Mesenchymal Stem Cells Attenuate Lipopolysaccharide-Induced Inflammatory Response in Human Uterine Smooth Muscle Cells. AJP Reports. 2020;10:e335-341. https://doi.org/10.1055/s-0040-1715166
Xiao B, Yang W, Lei D, Huang J, Yin Y, Zhu Y. PGS Scaffolds Promote the In Vivo Survival and Directional Differentiation of Bone Marrow Mesenchymal Stem Cells Restoring the Morphology and Function of Wounded Rat Uterus. Advanced Healthcare Materials. 2019;8:1801455. https://doi.org/10.1002/adhm.201801455
Jiang C, Centonze A, Song Y, Chrisnandy A, Tika E, Rezakhani S. Collagen signaling and matrix stiffness regulate multipotency in glandular epithelial stem cells in mice. Nature Communications. 2024; 15:10482. https://doi.org/10.1038/s41467-024-54843-5
An J, Ma T, Wang Q, Zhang J, Santerre JP, Wang W. Defining optimal electrospun membranes to enhance biological activities of human endometrial MSCs. Frontiers in Bioengineering and Biotechnology. 2025;13:1551791. https://doi.org/10.3389/fbioe.2025.1551791
Adamyan L, Pivazyan L, Krylova E, Kurbatova K, Tarlakyan V, Stepanian A. Hyaluronic acid in the prevention of adhesions after gynecological surgery: systematic review and meta-analysis. Journal of Endometriosis and Uterine Disorders. 2024;6:100070. https://doi.org/10.1016/j.jeud.2024.100070
Adamyan LV, Mikhaleva LM, Tkachev NA, Pivazyan LG, Vandysheva RA, Areshidze DA, Mailova KS. Modelling and prevention of postoperative adhesions in gynaecological and pelvic surgery in experiment: morphological and ultrastructural features. Russian Journal of Human Reproduction. 2024;30(2):42-50. (In Russ.). https://doi.org/10.17116/repro20243002142
Patent RF №RU 2839347 C1. Opubl. 12.03.2024. Adamyan LV, Mailova KS, Pivazyan LG, Mikhaleva LM, Vandysheva RA, Annurkina AI. Sposob profilaktiki posleoperatsionnykh peritonealnykh spayek v ginekologicheskoy i tazovoy khirurgii pri ikh eksperimentalnom modelirovanii u krys. (In Russ.)
Touki HD, Ahmed NU, Motalab M, Bose P. Atomistic investigations of mechanical properties and degradation rates of polycaprolactone blends with polylactic acid and polyglycolic acid. Materials Research Express. 2025;12:085307. https://doi.org/10.1088/2053-1591/adfad5
Reddy MSB, Ponnamma D, Choudhary R, Sadasivuni KK. A Comparative Review of Natural and Synthetic Biopolymer Composite Scaffolds. Polymers. 2021;13:1105. https://doi.org/10.3390/polym13071105
Park EJ, Guo J, Teo YC, Teo P. Biomolecule-modified synthetic polymers for wound healing and orthopaedic applications. RSC Applied Polymers. 2025;3:1124-1144. https://doi.org/10.1039/D5LP00074B
Fernández-Pérez J, Ahearne M. The impact of decellularization methods on extracellular matrix derived hydrogels. Scientific Reports. 2019;9:14933. https://doi.org/10.1038/s41598-019-49575-2
López-Martínez S, Rodríguez-Eguren A, de Miguel-Gómez L, Francés-Herrero E, Faus A, Díaz A. Bioengineered endometrial hydrogels with growth factors promote tissue regeneration and restore fertility in murine models. Acta Biomaterialia. 2021;135:113-125. https://doi.org/10.1016/j.actbio.2021.08.025
Abolhasani S, Ahmadi Y, Rostami Y, Baravar E, Fattahi D. Biomaterials in tissue repair and regeneration: key insights from extracellular matrix biology. Frontiers in Medical Technology. 2025;7:1565810. https://doi.org/10.3389/fmedt.2025.1565810
Chen S, Wang T, Chen J, Sui M, Wang L, Zhao X. 3D bioprinting technology innovation in female reproductive system. Materials Today Bio. 2025;31:101551. https://doi.org/10.1016/j.mtbio.2025.101551
Martyniak K, Lokshina A, Cruz MA, Karimzadeh M, Kemp R, Kean TJ. Biomaterial composition and stiffness as decisive properties of 3D bioprinted constructs for type II collagen stimulation. Acta Biomaterialia. 2022;152:221-234. https://doi.org/10.1016/j.actbio.2022.08.058
Dvorak N, Liu Z, Mouthuy P-A. Soft bioreactor systems: a necessary step toward engineered MSK soft tissue? Frontiers in Robotics and AI. 2024;11:1287446. https://doi.org/10.3389/frobt.2024.1287446
Liang S, Su Y, Yao R. 3D Bioprinting of Induced Pluripotent Stem Cells and Disease Modeling. Handbook of Experimental Pharmacology. 2023;281:29-56. https://doi.org/10.1007/164_2023_646
Ji W, Hou B, Lin W, Wang L, Zheng W, Li W, Zheng J, Wen X, He P. 3D Bioprinting a human iPSC-derived MSC-loaded scaffold for repair of the uterine endometrium. Acta biomaterialia. 2020;116:268-284. https://doi.org/10.1016/j.actbio.2020.09.012
Wen J, Hou B, Lin W, Guo F, Cheng M, Zheng J. 3D-printed hydrogel scaffold-loaded granulocyte colony-stimulating factor sustained-release microspheres and their effect on endometrial regeneration. Biomaterials Science. 2022;10:3346-3358. https://doi.org/10.1039/D2BM00109H
Luce E, Duclos-Vallee J-C. Stem Cells and Organoids: A Paradigm Shift in Preclinical Models toward Personalized Medicine. Pharmaceuticals. 2025;18:992. https://doi.org/10.3390/ph18070992
Cui Y, Zhao H, Wu S, Li X. Human Female Reproductive System Organoids: Applications in Developmental Biology, Disease Modelling, and Drug Discovery. Stem Cell Reviews and Reports. 2020; 16:1173-1184. https://doi.org/10.1007/s12015-020-10039-0
Zhang X, Zhang L, Li T, Zhang Z, Shang X, Bai H. Investigating bacteria-induced inflammatory responses using novel endometrial epithelial gland organoid models. Frontiers in Reproductive Health. 2024;6:1490520. https://doi.org/10.3389/frph.2024.1490520
Hwang S-Y, Lee D, Lee G, Ahn J, Lee Y-G, Koo HS. Endometrial organoids: a reservoir of functional mitochondria for uterine repair. Theranostics. 2024;14:954-972. https://doi.org/10.7150/thno.90538
Xu Y, Cai S, Wang Q, Cheng M, Hui X, Dzakah EE. Multi-Lineage Human Endometrial Organoids on Acellular Amniotic Membrane for Endometrium Regeneration. Cell Transplantation. 2023;32: 9636897231218408. https://doi.org/10.1177/09636897231218408
Schofield R. The relationship between the spleen colony-forming cell and the haemopoietic stem cell. Blood Cells. 1978;4(1-2):7-25.
Pal’tsev M.A. Stem Cells and Cellular Technologies: Present and Future. Remedium. 2006;8:6-13. (In Russ.).
Sukhikh GT, Malaitsev VV, Bogdanova IM, Dubrovina IV. Mezenkhimal’nye stvolovye kletki: Obzor. Biulleten’ eksperimental’noi biologii i meditsiny. 2002;133(2):124-131. (In Russ.).
Loewendorf A, Csete M. Concise review: immunologic lessons from solid organ transplantation for stem cell-based therapies. Stem Cells Translational Medicine. 2013;2(2):136-142. https://doi.org/10.5966/sctm.2012-0125
Adamyan LV, Pivazyan LG. Interdisciplinary approach and the current state of the issue of premature ovarian aging (literature review). Russian Journal of Human Reproduction. 2023;29(1):94-103. (In Russ.) https://doi.org/10.17116/repro20232901194