The creation of a model of chronic psoriasis-like dermatitis, the objectification of inflammation intensity evaluation are relevant tasks for pre-clinical studies.
OBJECTIVE
To evaluate the effect of external therapy on inflammation and subpopulational composition of immune skin cells in a new experimental model of psoriasis.
MATERIAL AND METHODS
Female C57BL/6 mice were investigated: group 1 (n=12) — mice with chronic psoriasis-like dermatitis without the administration of therapy using pathology inductor (5% imiquimod cream, 62.5 mg/cm2/day/mouse, 7 days); group 2 (experimental) (n=11) — mice with chronic psoriasis-like dermatitis, that received a course of external therapy with an emollient containing piroctone olamine, bisabolol, ceramide, cholesterol, phytosphingosine, glycerin, glyceryl stearate, in addition to a pathology inductor for 7 days; group 3 (n=11) — mice with a model of chronic psoriasis-like dermatitis, that received a course of external therapy with 1% hydrocortisone ointment in addition to a pathology inductor for 7 days; group 4 (n=10) — healthy mice without treatment. Severity of the skin inflammation was assessed on a rating scale, the skin biopsy samples were examined on the 7th day. The subpopulational structure of mononuclear cells (MNC) was evaluated by flow cytometry using monoclonal antibodies to the corresponding antigens (CD3, CD4, CD8, CD5, MHC class II, TCRγδ, CD38, CD80, CD83, CD86, TLR2).
RESULTS
The γδT lymphocytes and Langerhans cells (CD207+) proportion was most significantly changed. The use of therapy with hydrocortisone ointment has led to a normalization of the indicators, reduced inflammation signs (acanthosis, hyperkeratosis, papillomatosis, lymphocytic infiltration of the skin), complete absence of Munro’s microabscesses and reduction of skin thickness.
CONCLUSION
The evaluation criteria of the anti-inflammatory effect in the experimental model of psoriasis are reduction of γδT and CD207 cells content. The proposed method of therapeutic effectiveness assessment allows to perform a more accurate evaluation of anti-inflammatory effect in pre-clinical studies.
