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Sobolev V.V.

Mechnikov Research Institute of Vaccines and Sera;
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences

Melnichenko O.O.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Zhukova O.V.

Moscow Research and Practical Center for Dermatovenereology and Cosmetology, Department of Healthcare;
Peoples’ Friendship University of Russia

Potekaev N.N.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
The Russian National Research Medical University named after N.I. Pirogov

Korsunskaya I.M.

Center for Theoretical Problems of Physicochemical Pharmacology of Institution of Russian Academy of Sciences

Effect of low intensity laser irradiation on RORC gene expression level in psoriasis patients

Authors:

Sobolev V.V., Melnichenko O.O., Zhukova O.V., Potekaev N.N., Korsunskaya I.M.

More about the authors

Journal: Russian Journal of Clinical Dermatology and Venereology. 2022;21(5): 606‑609

DOI: 10.17116/klinderma202221051606

Views: 1079

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To cite this article:

Sobolev VV, Melnichenko OO, Zhukova OV, Potekaev NN, Korsunskaya IM. Effect of low intensity laser irradiation on RORC gene expression level in psoriasis patients. Russian Journal of Clinical Dermatology and Venereology. 2022;21(5):606‑609. (In Russ.)
https://doi.org/10.17116/klinderma202221051606

 
МСФ на ЯМ
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Orphan nuclear receptor γ, associated with the retinoic acid receptor (RORγ/RORC), controls the proinflammatory gene expression program, which is involved in the pathogenesis of several human immune-mediated diseases, including psoriasis.

OBJECTIVE

To analyze RORC gene expression in psoriatic plaques and unaffected skin of the same patients. To study the effect of 1.27 µm laser irradiation on RORC gene expression in the skin of psoriasis patients.

MATERIAL AND METHODS

The study was performed on skin biopsy specimens taken from 12 patients with psoriasis. RORC gene expression was analyzed by real-time PCR.

RESULTS

RORC gene expression in psoriatic plaques was significantly increased by an average of 8.16±2.06 times relative to unaffected skin of the same patients. We observed a significant decrease in RORC gene expression up to 3.21±1.23 times in psoriatic plaques after treatment of psoriasis patients with low-intensity laser irradiation.

CONCLUSION

High expression of the RORC gene in psoriatic plaques may indicate its important role in psoriasis, and a decrease in expression after exposure to laser irradiation allows its use as a molecular indicator of treatment efficacy.

Keywords:

psoriasis
RORC
gene expression
real-time PCR
low intensity laser irradiation

Authors:

Sobolev V.V.

Mechnikov Research Institute of Vaccines and Sera;
Center for Theoretical Problems of Physicochemical Pharmacology of the Russian Academy of Sciences

Melnichenko O.O.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology

Zhukova O.V.

Moscow Research and Practical Center for Dermatovenereology and Cosmetology, Department of Healthcare;
Peoples’ Friendship University of Russia

Potekaev N.N.

Moscow Scientific and Practical Center of Dermatovenerology and Cosmetology;
The Russian National Research Medical University named after N.I. Pirogov

Korsunskaya I.M.

Center for Theoretical Problems of Physicochemical Pharmacology of Institution of Russian Academy of Sciences

Received:

11.06.2021

Accepted:

23.07.2022

List of references:

  1. Fernandez-Obregon A. Psoriasis. In: O’Daly J, ed. Psoriasis — A Systemic Disease. InTech; 2012. https://doi.org/10.5772/25894
  2. Sobolev VV, Sautin MYe, Piruzyan AL, Soboleva AG, Korsunskaya IM. Common mechanisms and mediators of inflammation in atherosclerosis and psoriasis. Effektivnaya farmakoterapiya. Dermatovenerologiya i dermatokosmetologiya. 2017;2(15):16-19. (In Russ.).
  3. Klimov EA, Tretyakov AV, Gapanovich ES, Kokaeva ZG, Soboleva AG, Sakaniya LR, Korsunskaya IM, Sobolev VV. Evaluation of the role of polymorphic variants of NO-synthases genes of in pathogenesis of psoriasis. Molecular Medicine. 2018;4:16. (In Russ.). https://doi.org/10.29296/24999490-2018-04-11
  4. Klimov EA, Malakhova AV, Korobeinikova LA, Azimova YuE, Rudko OI, Kokaeva ZG, Korsunskaya IM, Sobolev VV. Polymorphic variants of the cholecystokinergic system genes: associations with panic disorders. Medical Council. 2018;12:190-194. (In Russ.). https://doi.org/10.21518/2079-701X-2018-12-190-194
  5. Sakaniya LR, Tretyakov AV, Shevtsova AA, Kokaeva ZG, Rudko OI, Soboleva AG, Korsunskaya IM, Klimov EA, Sobolev VV. DBH gene polymorphism in psoriasis patients. Molecular Medicine. 2019;17:62-65. (In Russ.). https://doi.org/10.29296/24999490-2019-04-09
  6. Sobolev V, Klimov E, Tretiakov A, Dvoryankova E, Denisova E, Denieva M, Korsunskaya I. 218 Polymorphism of dopamine related genes in the light of psychodermatology: Association with psoriasis. Journal of Investigative Dermatology. 2017;137(10)(Suppl 2):S230. https://doi.org/10.1016/j.jid.2017.07.215
  7. Sobolev VV, Mezentsev AV, Ziganshin RH, Soboleva AG, Denieva M, Korsunskaya IM, Svitich OA. LC-MS/MS analysis of lesional and normally looking psoriatic skin reveals significant changes in protein metabolism and RNA processing. PLoS One. 2021;16(5):e0240956.
  8. Klimov E, Tretiakov A, Gapanovich E, Kokaeva Z, Soboleva A, Sakaniya L, Korsunskaya I, Sobolev V. Assessment of the Role of NO Synthase Genes Polymorphisms in the Pathogenesis of Psoriasis. JAMMR. 2018;26:1-6.  https://doi.org/10.9734/JAMMR/2018/41039
  9. Sobolev V, Sakaniya L, Tretiakov A, Kokaeva Z, Naumova E, Rudko O, Soboleva A, Danilin I, Korsunskaya I, Klimov E. Association of GA genotype of SNP rs4680 in COMT gene with psoriasis. Arch Dermatol Res. 2019; 311:309-315.  https://doi.org/10.1007/s00403-019-01904-1
  10. Sobolev V, Nesterova A, Soboleva A, Dvoriankova E, Piruzyan A, Mildzikhova D, Korsunskaya I, Svitich O. The Model of PPARγ-Downregulated Signaling in Psoriasis. PPAR Research. 2020;2020:1-11.  https://doi.org/10.1155/2020/6529057
  11. Sobolev VV, Denisova EV, Korsunskaya IM. Alteration of STAT3 gene expression in psoriasis treatment. Medical Council. 2020;(12):71-74. (In Russ.). https://doi.org/10.21518/2079-701X-2020-12-71-74
  12. Ciofani M, Madar A, Galan C, Sellars M, Mace K, Pauli F, et al. A validated regulatory network for Th17 cell specification. Cell. 2012;151:289-303. 
  13. Takaishi M, Ishizaki M, Suzuki K, Isobe T, Shimozato T, Sano S. Oral administration of a novel RORγt antagonist attenuates psoriasis-like skin lesion of two independent mouse models through neutralization of IL-17. Journal of Dermatological Science. 2017;85:12-19.  https://doi.org/10.1016/j.jdermsci.2016.10.001
  14. Korn T, Bettelli E, Oukka M, Kuchroo VK. IL-17 and Th17 Cells. Annu Rev Immunol. 2009;27:485-517. 
  15. Livak KJ, Schmittgen TD. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2−ΔΔCT Method. Methods. 2001; 25(4):402-408.  https://doi.org/10.1006/meth.2001.1262
  16. Pandya VB, Kumar S, Sachchidanand, Sharma R, Desai RC. Combating Autoimmune Diseases With Retinoic Acid Receptor-Related Orphan Receptor-γ (RORγ or RORc) Inhibitors: Hits and Misses. J Med Chem. 2018; 61:10976-10995. https://doi.org/10.1021/acs.jmedchem.8b00588
  17. Castro G, Liu X, Ngo K, De Leon-Tabaldo A, Zhao S, Luna-Roman R, et al. RORγt and RORα signature genes in human Th17 cells. PLoS One. 2017;12: e0181868. https://doi.org/10.1371/journal.pone.0181868
  18. Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, et al. The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 2006;126(6):1121-1133.

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