Biorevitalization is a well-known and long-proven method for skin rejuvenation, which increases skin hydration, improves its turgor and density, increases elasticity, and replenishes facial volume. Despite the wide variety of fillers, they all differ in their characteristics and mechanism of action. The article describes the key stages of the physiological processes of natural skin aging and an innovative way to alter them using a combined preparation composed of non-reticulated high concentration hyaluronic acid, creating homogeneous and uniform integration into the tissues, and glycerin, which activates the aquaporin mechanism of moisture redistribution in the skin and provides a delay in biodegradation of hyaluronic acid. The combination of glycerin and hyaluronic acid restores normal skin hydration, strengthens the protective barrier under stress, and results in a double increase in the antioxidant potential of the skin. Biorevitalization with hyaluronic acid and glycerin-based preparation with optimal concentration rapidly restores skin turgor via uniform rehydration of the dermis and epidermis and allows long intervals between treatments.