The article is devoted to studying the change of physical and chemical properties of hyaluronic acid-based tissue fillers after focused ultrasound and radio-frequency spectrum exposure. The physical properties of tissue fillers (dynamic viscosity, water absorption, cohesiveness) before and after focused ultrasound exposure, as well as radio-frequency spectrum are described. Experiments were carried out in vitro in a polyethylene bag, as well as on a cadaver material, anatomical bioblocks of human origin at the Multidisciplinary Accreditation and Simulation Center of the Institute of Anatomy and Morphology named after Academician Yu. M. Lopukhin of the Pirogov RNRMU with the support of the MHICPE, companies producing hyaluronic acid-based tissue fillers, «National Company of Beauty» and KIT-MED companies. The obtained data show that the physical and chemical properties of hyaluronic acid-based tissue fillers have changed after hardware methods exposure. According to the ultrasound data, it is clearly seen that the volume of hyaluronic acid-based tissue filler decreases and its fragmentation occurs in some cases due to the impact of physical factors. This fact requires further investigation.