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Andreev-Andrievsky A.A.

Mitoengineering Research Institute of the Moscow State University;
Lomonosov Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences

Bolgarina A.A.

N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia

Manskikh V.N.

Mitoengineering Research Institute of the Moscow State University

Gabitov R.B.

A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia

Lagereva E.A.

Mitoengineering Research Institute of the Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences

Fadeeva O.V.

Mitoengineering Research Institute of the Moscow State University

Telyatnikova E.V.

Mitoengineering Research Institute of the Moscow State University

Shcherbakova V.S.

N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia

Mechanisms of the wound-healing action of native collagen type I in ischemic model full-thickness skin wounds on the example — medical devices Collost «(part I)

Authors:

Andreev-Andrievsky A.A., Bolgarina A.A., Manskikh V.N., Gabitov R.B., Lagereva E.A., Fadeeva O.V., Telyatnikova E.V., Shcherbakova V.S.

More about the authors

Journal: Pirogov Russian Journal of Surgery. 2020;(10): 79‑87

DOI: 10.17116/hirurgia202010179

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MECHANISMS OF THE WOUND-HEALING ACTION OF NATIVE COLLAGEN TYPE I IN ISCHEMIC MODEL FULL-THICKNESS SKIN WOUNDS ON THE EXAMPLE — MEDICAL DEVICES COLLOST «(PART I)
MECHANISMS OF THE WOUND-HEALING ACTION OF NATIVE COLLAGEN TYPE I IN ISCHEMIC MODEL FULL-THICKNESS SKIN WOUNDS ON THE EXAMPLE — MEDICAL DEVICES COLLOST «(PART I)

To cite this article:

Andreev-Andrievsky AA, Bolgarina AA, Manskikh VN, et al. . Mechanisms of the wound-healing action of native collagen type I in ischemic model full-thickness skin wounds on the example — medical devices Collost «(part I). Pirogov Russian Journal of Surgery. 2020;(10):79‑87. (In Russ.)
https://doi.org/10.17116/hirurgia202010179

Подписка 2026 Хирургия. Журнал им. Н.И. Пирогова (Pirogov Russian Journal of Surgery)
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Abstract:

Active collagen type I successfully used in regenerative medicine. However, despite the large amount of material of cellular and molecular mechanisms underlying skin repair, the molecular mechanisms of wound healing with use collagen type I, not studied enough.

PURPOSE OF THE STUDY

To study the mechanism of the native collagen type I wound-healing action of native type I collagen on the example of the medical device Collost (7% gel) in a model of the rats difficult-to-heal skin wounds.

MATERIAL AND METHODS

Male rats in population SD (72 individuals) surgically formed an ischemic dorsal skin flap (3×10 cm) with two full-thickness skin wounds 6 mm in diameter.The trained animals divided into 2 groups: in the experimental group, medical device Collost (gel) applied once after the operation, in the control group — a standard medical device for comparison. The dynamics of wound healing assessed, the number of M2 macrophages, myofibroblasts, vascularization and expression of the main markers of the repair process in the wound tissues and time points for assessment were: after 3, 7 and 14 days after operation using macroscopic, immunohistochemical, and molecular methods.

RESULTS:

It has been established that the mechanism of action of native collagen type I is associated with the acceleration of the appearance of «progenitorous» M2-macrophages in the wound tissues, decrease in the severity of inflammation or reduction in the duration of the inflammatory stage of the repair process, change in the expression spectrum of number of growth factors, an acceleration of neovasculogenesis.

CONCLUSION

In this work, on the modern experimental model shown regenerative efficiency of a medical device based on collagen type I and described the molecular and cellular processes of wound healing when using it It has been shown that the acceleration of wound healing processes occurs when using a medical device based on native collagen type 1, it is also accompanied by a better aesthetic closure of the damaged skin area.

Keywords:

Collagen type I
Collost
chronic wounds
M2-macrophages
fibroblasts
wound healing
vascularization
cytokines

Authors:

Andreev-Andrievsky A.A.

Mitoengineering Research Institute of the Moscow State University;
Lomonosov Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences

Bolgarina A.A.

N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia

Manskikh V.N.

Mitoengineering Research Institute of the Moscow State University

Gabitov R.B.

A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia

Lagereva E.A.

Mitoengineering Research Institute of the Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences

Fadeeva O.V.

Mitoengineering Research Institute of the Moscow State University

Telyatnikova E.V.

Mitoengineering Research Institute of the Moscow State University

Shcherbakova V.S.

N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia

Received:

05.08.2020

Accepted:

03.09.2020

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References:

  1. Hochstein AO, DPM & Bhatia A, DPM. Collagen : its role in wound healing. Podiatry Manag. 2014;103-106:109-110. 
  2. Singer AJ, Clark RAF. Cutaneous wound healing. New England Journal of Medicine. 1999;341:10:738-746.  https://doi.org/10.1056/NEJM199909023411006
  3. Diegelmann RF, Evans MC. Wound healing: An overview of acute, fibrotic and delayed healing. Frontiers in Bioscience. 2004;9:283-289.  https://doi.org/10.2741/1184
  4. Rodrigues M, Kosaric N, Bonham CA, Gurtner GC. Wound healing: A cellular perspective. Physiological Reviews. 2019;1(99):665-706.  https://doi.org/10.1152/physrev.00067.2017
  5. Tonnesen MG, Feng X, Clark RAF. Angiogenesis in wound healing. Journal of Investigative Dermatology Symposium Proceedings. 2000;1(5):40-46.  https://doi.org/10.1046/j.1087-0024.2000.00014.x
  6. Koh TJ, DiPietro LA. Inflammation and wound healing: the role of the macrophage. Expert reviews in molecular medicine. 2011;13.  https://doi.org/10.1017/s1462399411001943
  7. Krzyszczyk P, Schloss R, Palmer A, Berthiaume F. The role of macrophages in acute and chronic wound healing and interventions to promote pro-wound healing phenotypes. Frontiers in Physiology. 2018;9:419.  https://doi.org/10.3389/fphys.2018.00419
  8. Hesketh M, Sahin KB, West ZE, Murray RZ. Macrophage phenotypes regulate scar formation and chronic wound healing. International Journal of Molecular Sciences. 2017;18:7:1545. https://doi.org/10.3390/ijms18071545
  9. Morton LM, Phillips TJ. Wound healing and treating wounds Differential diagnosis and evaluation of chronic wounds. Journal of the American Academy of Dermatology. 2016;74:4:589-605.  https://doi.org/10.1016/j.jaad.2015.08.068
  10. Komarov AN, Kezina LP, Silina EV, Orlova AS, Koreyba KA. The clinical effectiveness of bioplastic material based on type i collagen for treatment of pressure ulcers in neurorehabilitaion patients: randomized comparative study. Vestnik vosstanovitel’noj mediciny. 2017;2:74-83. (In Russ.).
  11. Silina EV, Stupin VA, Gabitov RB. Collagen role in the mechanisms of chronic wounds healing diabetic foot syndrome. Klin med. 2018;2(96):106-115. (In Russ.).
  12. Zhang P, Lu J, Jing Y, Tang S, Zhu D, Bi Y. Global epidemiology of diabetic foot ulceration: a systematic review and meta-analysis. Annals of Medicine. 2017;49:2:106-116.  https://doi.org/10.1080/07853890.2016.1231932
  13. Moor AN, Tummel E, Prather JL, Jung M, Lopez JJ, Connors S, Gould LJ. Consequences of age on ischemic wound healing in rats: Altered antioxidant activity and delayed wound closure. Age. 2014;2(36):733-748.  https://doi.org/10.1007/s11357-014-9617-4
  14. Schrementi ME, Ranzer MJ, DiPietro LA. Impaired Wound Repair and Delayed Angiogenesis Springer Berlin Heidelberg. 2017;1003-1015. https://doi.org/10.1007/978-3-662-47398-6_85
  15. Dibirov MD, Gadzhimuradov RU, Khamitov FF, Polyansky MV, Tereshchenko SA. Application of modern technologies in treatment of purulonecrotic complications of diabetic foot syndrome. Serdechnaja i sosudistaja hirurgija. 2016;2:60-70. (In Russ.).
  16. Manturova NE, Stenko AG, Petinati YaA, Chaikovskaya EA, Bolgarina AA. Injectable collagen in correction of age-related skin changes: experimental and clinical parallels. Bulletin of Russian State Medical University. 2019;1:71-77. (In Russ.).
  17. Safojan AAN. Kollagenoplastika povrezhdenij kozhi. SPb.: SpecLit; 2019. (In Russ.).
  18. Seliverstov DV, Kuznetsov AV, Massevnin VV, Kondrus IV, Novikov LA, Yudin VA, Sazhin VP. The application of the «collost» biomaterial in the complex surgical treatment of degrees IV. RMZh. 2015;13(23):776-780. (In Russ.).
  19. Silina EV, Stupin VA, Zolotareva LS, Komarov AN. Native collagen application in clinical practice for chronic wounds treatment. Hirurgija. Zhurnal im. N.I. Pirogova. 2017;9:78-84. (In Russ.). https://doi.org/10.17116/hirurgia2017978-84
  20. Stupin VA, Silina EV, Gorskij VA, Gorjunov SV, Zhidkih SYu, Komarov AN, Sivkov AS, Gabitov RB, Zolotareva LS, Sinel’nikova TG, Barancevich ER, Bogomolov MS, Korejba KA, Bogdanov EA, Krivihin VT, Bakunov MYu, Eliseeva ME, Krivihin DV. Efficacy and safety of collagen biomaterial local application in complex treatment of the diabetic foot syndrome (final results of the multicenter randomised study). Hirurgija. Zhurnal im. N.I. Pirogova. 2018;6:91-100. (In Russ.). https://doi.org/10.17116/hirurgia2018691-100
  21. Dibirov M, Gadzhimuradov R, Koreiba K, Minabutdinov A. Biomedical Technologies in the Treatment of Skin and Soft Tissue Defects in Patients with Diabetic Foot Syndrome. International Journal of Biomedicine. 2016;6:41-45.  https://doi.org/10.21103/article6(1)_oa8
  22. Stupin VA, Gabitov RB, Sinelnikova TG, Silina EV. Biological mechanisms of chronic wound and diabetic foot healing: The role of collagen. Serbian Journal of Experimental and Clinical Research. 2018;19:4:373-382.  https://doi.org/10.2478/sjecr-2018-0077
  23. Godwin J, Kuraitis D, Rosenthal N. Extracellular matrix considerations for scar-free repair and regeneration: Insights from regenerative diversity among vertebrates. International Journal of Biochemistry and Cell Biology. 2014;56:47-55.  https://doi.org/10.1016/j.biocel.2014.10.011
  24. Chattopadhyay S, Raines RT. Review collagen-based biomaterials for wound healing. Biopolymers. 2014;101:8:821-833.  https://doi.org/10.1002/bip.22486
  25. Karr JC, Taddei AR, Picchietti S, Gambellini G, Fausto AM, Giorgi F. A morphological and biochemical analysis comparative study of the collagen products Biopad, Promogram, Puracol, and Colactive. Advances in skin & wound care. 2011;5(24):208-216.  https://doi.org/10.1097/01.asw.0000397897.18003.ce
  26. Neve A, Cantatore FP, Maruotti N, Corrado A, Domenico Ribatti D. Extracellular matrix modulates angiogenesis in physiological and pathological conditions. BioMed Research International. 2014. https://doi.org/10.1155/2014/756078
  27. Parenteau-Bareil R, Gauvin R, Cliché S, Gariépy C, Germain L, Berthod F. Comparative study of bovine, porcine and avian collagens for the production of a tissue engineered dermis. Acta Biomaterialia. 2011;10(7):3757-3765. https://doi.org/10.1016/j.actbio.2011.06.020
  28. Wiegand C, Schönfelder U, Abel M, Ruth P, Kaatz M, Hipler U-C. Protease and pro-inflammatory cytokine concentrations are elevated in chronic compared to acute wounds and can be modulated by collagen type i in vitro. Archives of Dermatological Research. 2010;6(302):419-428.  https://doi.org/10.1007/s00403-009-1011-1
  29. Wiegand C, Buhren BA, Bünemann E, Schrumpf H, Homey B, Frykberg RG, Lurie F, Gerber PA. A novel native collagen dressing with advantageous properties to promote physiological wound healing. Journal of Wound Care. 2016;12(25):713-720.  https://doi.org/10.12968/jowc.2016.25.12.713
  30. Schönfelder U, Abel M, Wiegand C, Klemm D, Elsner P, Hipler U-C. Influence of selected wound dressings on PMN elastase in chronic wound fluid and their antioxidative potential in vitro. Biomaterials. 2005;33(26):6664-6673. https://doi.org/10.1016/j.biomaterials.2005.04.030
  31. Kalmykova NV, Andreev-Andrievsky AA, Demyanenko IA, Manskikh VN, Lagereva EA,Popova AS,Khats YuS,Suslov AP. The stimulating effect of various forms of collagenic wound coverings on the epithelization process of skin wounds. Biomedicina. 2017;4:85-96. (In Russ.).
  32. Gould LJ, Leong M, Sonstein J, Wilson S. Optimization and validation of an ischemic wound model. Wound Repair and Regeneration. 2005;6(13):576-582.  https://doi.org/10.1111/j.1524-475x.2005.00080.x
  33. Trujillo AN, Kesl SL, Sherwood J, Wu M, Gould LJ. Demonstration of the rat ischemic skin wound model. Journal of Visualized Experiments. 2015;98:e52637. https://doi.org/10.3791/52637
  34. Conover WJ, Iman RL. Rank Transformations as a Bridge Between Parametric and Nonparametric Statistics. The American Statistician. 1981;3(35):124-129.  https://doi.org/10.1080/00031305.1981.10479327
  35. Crane MJ, Daley JM, van Houtte O, Brancato SK, Henry WL, Albina JE. The monocyte to macrophage transition in the murine sterile wound. PLoS ONE. 2014;1(9):e86660. https://doi.org/10.1371/journal.pone.0086660
  36. Mirza RE, Fang MM, Ennis WJ, Koh TJ. Blocking interleukin-1β induces a healing-associated wound macrophage phenotype and improves healing in type 2 diabetes. Diabetes. 2013;7(62):2579-2587. https://doi.org/10.2337/db12-1450
  37. Deonarine K, Panelli MC, Stashower ME, Jin P, Smith K, Slade HB, Norwood C, Wang E, Marincola FM& Stroncek DF. Gene expression profiling of cutaneous wound healing. Journal of Translational Medicine. 2007;1(5):11.  https://doi.org/10.1186/1479-5876-5-11
  38. Kotwal GJ, Chien S. Macrophage differentiation in normal and accelerated wound healing. Springer Verlag. 2017;353-364.  https://doi.org/10.1007/978-3-319-54090-0_14
  39. Gilbert RWD, Vickaryous MK, Viloria-Petit AM. Signalling by transforming growth factor beta isoforms in wound healing and tissue regeneration. Journal of Developmental Biology. 2016;4:2:21.  https://doi.org/10.3390/jdb4020021
  40. Penn JW, Grobbelaar AO, Rolfe KJ. The role of the TGF-β family in wound healing, burns and scarring: a review. International journal of burns and trauma. 2012;1(2):18-28. 
  41. Chu Y, Guo F, Li Y, Li X, Zhou T, Guo Y. A novel truncated TGF-β receptor II downregulates collagen synthesis and TGF-β I secretion of keloid fibroblasts. Connective Tissue Research. 2008;2(49):92-98.  https://doi.org/10.1080/03008200801913924
  42. Cutroneo KR. TGF-β-induced fibrosis and SMAD signaling: Oligo decoys as natural therapeutics for inhibition of tissue fibrosis and scarring. Wound Repair and Regeneration. 2007;15(suppl 1):54-60.  https://doi.org/10.1111/j.1524-475x.2007.00226.x
  43. Ho JD, Chung HJ, Ms Barron A, Ho DA, Sahni D, Browning JL, Bhawan J. Extensive CD34-to-CD90 Fibroblast Transition Defines Regions of Cutaneous Reparative, Hypertrophic, and Keloidal Scarring. American Journal of Dermatopathology. 2019;1(41):16-28.  https://doi.org/10.1097/DAD.0000000000001254
  44. Krzystek-Korpacka M, Kędzior K, Masłowski L, Mierzchala M, Bednarz-Misa I, Bronowicka-Szydelko A, Kubiak J, Gacka M, Placzkowska S, Gamian A. Impact of chronic wounds of various etiology on systemic profiles of key inflammatory cytokines, chemokines and growth factors, and their interplay. Advances in Clinical and Experimental Medicine. 2019;10(28):1301-1309. https://doi.org/10.17219/acem/103845
  45. Somasundaram R, Schuppan D. Type I, II, III, IV, V, and VI collagens serve as extracellular ligands for the isoforms of platelet-derived growth factor (AA, BB, and AB). Journal of Biological Chemistry. 1996;43(271):26884-26891. https://doi.org/10.1074/jbc.271.43.26884
  46. Felician FF, Yu R-H, Li M-Z, Li C-J, Chen H-Q, Jiang Y, Tang T, Qi W-Y, Xu H-M. The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum. Chinese Journal of Traumatology — English Edition. 2019;1(22):12-20.  https://doi.org/10.1016/j.cjtee.2018.10.004
  47. Misiura M, Miltyk W. Proline-containing peptides — New insight and implications: A Review. BioFactors. 2019;45:6:857-866.  https://doi.org/10.1002/biof.1554
  48. Godwin J. The promise of perfect adult tissue repair and regeneration in mammals: Learning from regenerative amphibians and fish. BioEssays. 2014;9(36):861-871.  https://doi.org/10.1002/bies.201300144
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