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Andreev-Andrievsky A.A.
Mitoengineering Research Institute of the Moscow State University;
Lomonosov Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences
Bolgarina A.A.
N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia
Manskikh V.N.
Mitoengineering Research Institute of the Moscow State University
Gabitov R.B.
A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia
Lagereva E.A.
Mitoengineering Research Institute of the Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences
Fadeeva O.V.
Mitoengineering Research Institute of the Moscow State University
Telyatnikova E.V.
Mitoengineering Research Institute of the Moscow State University
Shcherbakova V.S.
N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia
Mechanisms of the wound-healing action of native collagen type I in ischemic model full-thickness skin wounds on the example — medical devices Collost «(part I)
Journal: Pirogov Russian Journal of Surgery. 2020;(10): 79‑87
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To cite this article:
Andreev-Andrievsky AA, Bolgarina AA, Manskikh VN, et al. . Mechanisms of the wound-healing action of native collagen type I in ischemic model full-thickness skin wounds on the example — medical devices Collost «(part I). Pirogov Russian Journal of Surgery.
2020;(10):79‑87. (In Russ.)
https://doi.org/10.17116/hirurgia202010179
Active collagen type I successfully used in regenerative medicine. However, despite the large amount of material of cellular and molecular mechanisms underlying skin repair, the molecular mechanisms of wound healing with use collagen type I, not studied enough.
To study the mechanism of the native collagen type I wound-healing action of native type I collagen on the example of the medical device Collost (7% gel) in a model of the rats difficult-to-heal skin wounds.
Male rats in population SD (72 individuals) surgically formed an ischemic dorsal skin flap (3×10 cm) with two full-thickness skin wounds 6 mm in diameter.The trained animals divided into 2 groups: in the experimental group, medical device Collost (gel) applied once after the operation, in the control group — a standard medical device for comparison. The dynamics of wound healing assessed, the number of M2 macrophages, myofibroblasts, vascularization and expression of the main markers of the repair process in the wound tissues and time points for assessment were: after 3, 7 and 14 days after operation using macroscopic, immunohistochemical, and molecular methods.
It has been established that the mechanism of action of native collagen type I is associated with the acceleration of the appearance of «progenitorous» M2-macrophages in the wound tissues, decrease in the severity of inflammation or reduction in the duration of the inflammatory stage of the repair process, change in the expression spectrum of number of growth factors, an acceleration of neovasculogenesis.
In this work, on the modern experimental model shown regenerative efficiency of a medical device based on collagen type I and described the molecular and cellular processes of wound healing when using it It has been shown that the acceleration of wound healing processes occurs when using a medical device based on native collagen type 1, it is also accompanied by a better aesthetic closure of the damaged skin area.
Keywords:
Authors:
Andreev-Andrievsky A.A.
Mitoengineering Research Institute of the Moscow State University;
Lomonosov Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences
Bolgarina A.A.
N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia
Manskikh V.N.
Mitoengineering Research Institute of the Moscow State University
Gabitov R.B.
A.I. Evdokimov Moscow State University of Medicine and Dentistry of the Ministry of Health of Russia
Lagereva E.A.
Mitoengineering Research Institute of the Moscow State University;
State Scientific Center of the Russian Federation — Institute of Medical and Biological Problems of the Russian Academy of Sciences
Fadeeva O.V.
Mitoengineering Research Institute of the Moscow State University
Telyatnikova E.V.
Mitoengineering Research Institute of the Moscow State University
Shcherbakova V.S.
N.F. Gamaleya National Research Center for Epidemiology and Microbiology of the Ministry of Health of Russia
Received:
05.08.2020
Accepted:
03.09.2020
List of references:
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