BACKGROUND

The combined effect of cultured dermal fibroblasts and surfactant will provide new knowledge about the healing of trophic ischemic skin ulcers.

OBJECTIVE

To study the morphological features of biopsy samples of a healing ischemic skin defect in mice after transplantation of a tissue-engineered construct from pulmonary surfactant and dermal fibroblasts.

MATERIAL AND METHODS

Using hematoxylin and eosin staining of paraffin sections of biopsy samples of an ischemic skin defect in 145 mature C57/B1 mice, divided into control and experimental groups, in which an ischemic skin wound was modeled using MS Office Excel 2007 and STATISTICA 10.0 Enterprise, studied the thickness of the epidermis and the specific area of granulation tissue components on the 4th, 7th, 10th, 12th and 15th days of reparative histogenesis.

RESULTS

It was found that by the 15th day, the granulation tissue of biopsy specimens from mice in the experimental group had entered the third stage of the wound process, while in mice without treatment the second stage of the wound process continued. The thickness of the epidermis and the specific area occupied by collagen fibers in biopsy specimens increases and remains consistently higher after transplantation of a tissue-engineered construct from pulmonary surfactant and dermal allofibroblasts. In the experimental group, the maximum increase in the area occupied by collagen fibers is present between the 4th and 7th days, and in the control group - between the 12th and 15th days. Vascularization of granulation tissue increases without treatment, and when using an allofibroblast culture with the support of surfactant, it decreases by the 15th day of regeneration, which indicates fibrosis of the granulation tissue of biopsy specimens.

CONCLUSION

After transplantation into the wound, the surfactant in combination with dermal allofibroblasts accelerates the healing of an ischemic skin defect by 13.69%.