Increase in number of multiresistant and highly-virulent strains leads to the necessity of search of alternative antimicrobial agents. Antimicrobial effect ofphotosensitizers, light bulb and both altogether on hospital strains by the way of photodynamic reaction modeling has been studied. Photosensitizer Photosens in various concentrations was used. Light bulb «Biospek» with emission power 40 Wt/cm2 and wave-length 660—680 nanometres were used. Maximal antimicrobial effect was achieved in employment in common of photosensitizer and beaming with identification of optimal time of beaming of sensitized bacteria.