The experience of laparoscopic cholecystectomy (LCE) in women with gallstone disease, chronic gallstone cholecystitis with frequent episodes of biliary colic during the second trimester of pregnancy is presented. Original method of trocar insertion through the rectus abdominis muscle is proposed enabling to safely extract the gallbladder. Proposed double-access laparoscopic cholecystectomy allows reducing surgical trauma to the abdominal wall. Data submitted offer the possibility of laparoscopic cholecystectomy in pregnant with gallstone disease, chronic gallstone cholecystitis with frequent episodes of biliary colic which enables to preserve advantages of endoscopic surgery, accomplish necessary extent of operation, reduce patient's hospital stay, improve outcomes of pregnancy for mother and fetus and enhance quality of life. LCE is the operation of choice in pregnant and is reasonable after 15-16 weeks of gestation.