Application of atomic force microscopy for the comparative analysis of moderate and severe acute pancreatitis in the early stages of the disease

Gulikyan G.N.; Pakhomova R.A.; Kozlov V.V.

doi:https://doi.org/10.17116/dokgastro20251403129

Gulikyan G.N.

Clinical Hospital “RZD-Medicine

ORCID: 0000-0002-1549-0319

Pakhomova R.A.

Russian Biotechnological University

ORCID: 0000-0002-3681-4685

Kozlov V.V.

Sechenov First Moscow State Medical University Ministry of Health of the Russian Federation (Sechenov University)

ORCID: 0000-0003-3941-2742

Application of atomic force microscopy for the comparative analysis of moderate and severe acute pancreatitis in the early stages of the disease

Authors:

Gulikyan G.N., Pakhomova R.A., Kozlov V.V.

More about the authors

Journal: Russian Journal of Evidence-Based Gastroenterology. 2025;14(3): 29‑34

DOI: 10.17116/dokgastro20251403129

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To cite this article:

Gulikyan GN, Pakhomova RA, Kozlov VV. Application of atomic force microscopy for the comparative analysis of moderate and severe acute pancreatitis in the early stages of the disease. Russian Journal of Evidence-Based Gastroenterology. 2025;14(3):29‑34. (In Russ.)
https://doi.org/10.17116/dokgastro20251403129

Подписка 2026 Доказательная гастроэнтерология (Russian Journal of Evidence-Based Gastroenterology)

Использование инфографики авторами научных работ

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OBJECTIVE

To compare the condition of erythrocytes and erythrocyte membranes in cases of moderate and severe acute pancreatitis during the early stages of the disease.

MATERIAL AND METHODS

A multicenter prospective study was conducted involving 15 practically healthy individuals and 61 patients with acute pancreatitis who were hospitalized in Krasnoyarsk from 2021 to 2023. Among them, 29 patients had clinically confirmed moderate acute pancreatitis, while 32 had severe acute pancreatitis. In addition to routine diagnostic methods, all participants underwent atomic force microscopy of erythrocytes collected from the cubital vein upon admission.

RESULTS

Examination of the erythrocyte membrane revealed the presence of erosions on the cell surface. Both the number and size of these ulcerative defects correlated with disease severity. In severe cases, the number of erosions approximately doubled, accompanied by an increase in their length, width, and depth. The greater the extent of these structural alterations, the higher the likelihood of erythrocyte destruction.

CONCLUSION

Given the similarity between erythrocyte membrane alterations and cellular changes in other organs, including the pancreas, atomic force microscopy may be recommended as a method of choice for assessing disease severity and predicting the progression of acute pancreatitis at early stages.

Keywords:

acute pancreatitis

erythrocyte membrane

erosion

atomic force microscopy

Authors:

Gulikyan G.N.

Clinical Hospital “RZD-Medicine

ORCID: 0000-0002-1549-0319

Pakhomova R.A.

Russian Biotechnological University

ORCID: 0000-0002-3681-4685

Kozlov V.V.

Sechenov First Moscow State Medical University Ministry of Health of the Russian Federation (Sechenov University)

ORCID: 0000-0003-3941-2742

Received:

15.08.2024

Accepted:

18.12.2024

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