The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2026
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Kazeko L.A.

Belarusian State Medical University

Zakharava V.A.

Belarusian State Medical University

Anfinogenova E.A.

Belarusian State Medical University

Cherstvoy E.D.

Belarusian State Medical University

The significance of the expression of matrix metalloproteinases in the differential diagnosis of periodontal diseases

Authors:

Kazeko L.A., Zakharava V.A., Anfinogenova E.A., Cherstvoy E.D.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2021;83(3): 20‑29

DOI: 10.17116/patol20218303120

Read: 6436 times

Download PDF (RU)
Contact the author
Table of contents

THE SIGNIFICANCE OF THE EXPRESSION OF MATRIX METALLOPROTEINASES IN THE DIFFERENTIAL DIAGNOSIS OF PERIODONTAL DISEASES
THE SIGNIFICANCE OF THE EXPRESSION OF MATRIX METALLOPROTEINASES IN THE DIFFERENTIAL DIAGNOSIS OF PERIODONTAL DISEASES

To cite this article:

Kazeko LA, Zakharava VA, Anfinogenova EA, Cherstvoy ED. The significance of the expression of matrix metalloproteinases in the differential diagnosis of periodontal diseases. Russian Journal of Archive of Pathology. 2021;83(3):20‑29. (In Russ.)
https://doi.org/10.17116/patol20218303120

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
Quality control of immu­nohistochemical studies: compulsory duty or urgent need?. Russian Journal of Archive of Pathology. 2025;(2):43-52
Placenta increta into the scar in combination with high-grade squamous intraepithelial lesion of the cervix. Russian Journal of Archive of Pathology. 2025;(2):64-68
Soli­tary fibrous tumor of the kidney. Russian Journal of Archive of Pathology. 2025;(3):77-81
Features of changes in hormone rece­ptor expression during therapy in women of reproductive age with hype­rplastic processes in the endo­metrium. Russian Bulletin of Obstetrician-Gynecologist. 2025;(2):21-28
Asso­ciations of gene­tic poly­morphisms in key inflammatory response genes with the risk of chro­nic apical periodontitis deve­lopment. Russian Journal of Stomatology. 2025;(2):52-56
Rapi­dly progressive periodontitis. (A successful expe­rience of eighteen years of obse­rvation). Russian Journal of Stomatology. 2025;(2):61-67
Practical aspe­cts of clinical and labo­ratory trials of medi­cal devi­ces for immu­nohistochemical studies. Communication 1. Trials of the instrument for immu­nohistochemical staining. Labo­ratory Service. 2025;(2):11-18
Targeted bone rege­neration in the treatment of patients with chro­nic gene­ralized periodontitis. Stomatology. 2025;(4):33-36
Morphological and immu­nohistochemical characteristics of skin specimens after isolated and combined inje­ctions of poly-L-lactic acid and hyaluronic acid. Plastic Surgery and Aesthetic Medi­cine. 2025;(3):50-62
Medi­cal and social characteristics of periodontal and oral mucosa prevalence in children from substitute fami­lies. Russian Journal of Stomatology. 2025;(3):69-73
Abstract:

Investigation of features of expression of matrix metalloproteinases (MMPs) is important for both understanding the mechanisms of the pathogenesis of periodontal diseases, and determining the nature of their course in order to choose a correct and timely treatment strategy.

OBJECTIVE

To establish the value of MMPs for the diagnosis and determination of the nature of the course of periodontitis at the stage of disease manifestation, by morphometrically assessing the expression of MMPs in the gingival biopsy material.

MATERIAL AND METHODS

Gingival biopsy specimens from 82 patients with rapidly progressing (n=26), chronic simple (n=18), and chronic complex (n=38) periodontitis were analyzed. Morphometric and statistical analysis of the expression of MMPs was carried out using Aperio ImageScope v. 12.4.0.5043, Statistica 10.0, and MedCalc (p<0.05).

RESULTS

Analysis of the nature of MMP expression in the gingival biopsy material of patients with periodontal diseases showed that MMP2 and MMP13 were approximately equally involved in the development and course of all the studied forms of periodontitis. An increase in the expression of MMP1, MMP8, and MMP14 and a decrease in that of MMP9 are of the greatest importance in the pathogenesis of a rapidly progressing process. The performed ROC analysis confirmed the significance of the parameters of general and stromal expression of MMP1, MMP9, and MMP14, and mainly stromal expression of MMP8 for the differential diagnosis of rapidly progressing periodontitis with chronic forms, including chronic complex periodontitis.

CONCLUSION

The expression indicators of MMR1, MMR8, MMR9, and MMR14 are most informative in determining the course of periodontitis at the stage of disease manifestation and differential diagnosis of rapidly progressing periodontitis with chronic simplex and complex periodontitis.

Keywords:

periodontitis
matrix metalloproteinase
immunohistochemistry
expression

Authors:

Kazeko L.A.

Belarusian State Medical University

Zakharava V.A.

Belarusian State Medical University

Anfinogenova E.A.

Belarusian State Medical University

Cherstvoy E.D.

Belarusian State Medical University

Received:

28.01.2021

Accepted:

05.03.2021

Close metadata

References:

  1. Navlet KP, Nisha KJ, Sanjeela G, Shyam P. Role of matrix metalloproteinases in periodontal disease — a review. Biomed J Sci Tech Res. 2018;2(1):2099-2104.
  2. Cavalla F, Hernandez-Ríos P, Sorsa T, Biguetti C, Hernandez M. Matrix metalloproteinases as regulators of periodontal inflammation. Int J Mol Sci. 2017;18(2):440.  https://doi.org/10.3390/ijms18020440
  3. Sorsa T, Mantyla P, Tervahartiala T, Pussinen PJ, Gamonal J, Hernandez M. MMP activation in diagnostics of periodontitis and systemic inflammation. J Clinl Periodontol. 2011;38(9):817-819.  https://doi.org/10.1111/j.1600-051X.2011.01753.x
  4. Hernandez M, Valenzuela MA, Lopez-Otin C, Alvarez J, Lopez JM, Vernal R, Gamonal J. Matrix metalloproteinase-13 is highly expressed in destructive periodontal disease activity. J Periodontol. 2006;77(11):1863-1870. https://doi.org/10.1902/jop.2006.050461
  5. Hernandez RM, Sorsa T, Obregon F, Tervahartiala T, Valenzuela MA, Pozo P, Dutzan N, Lesaffre E, Molas M, Gamonal J. Proteolytic roles of matrix metalloproteinase (MMP)-13 during progression of chronic periodontitis: Initial evidence for MMP-13/MMP-9 activation cascade. J Clin Periodontol. 2009;36(12):1011-1017. https://doi.org/10.1111/j.1600-051x.2009.01488.x
  6. Cox SW, Eley BM, Kiili M, Asikainen A, Tervahartiala T, Sorsa T. Collagen degradation by interleukin-1b-stimulated gingival fibroblasts is accompanied by release and activation of multiple matrix metalloproteinases and cysteine proteinases. Oral Dis. 2006;12(1):34-40.  https://doi.org/10.1111/j.1601-0825.2005.01153.x
  7. Sorsa T, Tjaderhane L, Konttinen Y, Lauhio A, Salo T, Lee H, Golub LM, Brown D L, Mantyla P. Matrix metalloproteinases: contribution to pathogenesis, diagnosis and treatment of periodontal inflammation. Ann Med. 2006;38(5):306-321.  https://doi.org/10.1080/07853890600800103
  8. Marcaccini AM, Meschiari CA, Zuardi LR, Sousa T, Taba M, Teofilo JM, Jacob-Ferreira AL, Tanus-Santos JE, Novaes AB, Gerlach RF. Gingival crevicular fluid levels of MMP-8, MMP-9, TIMP-2, and MPO decrease after periodontal therapy. J Clin Periodontol. 2010;37(2):180-190.  https://doi.org/10.1111/j.1600-051X.2009.01512.x
  9. Goncalves RP, Damante CA, Lima FL, Imbronito AV, Nunes FD, Pustiglioni FE. Detection of MMP-2 and MMP-9 salivary levels in patients with chronic periodontitis before and after periodontal treatment. Revista Odonto Ciencia. 2009;24(3):264-269. 
  10. Scott DA, Krauss J. Neutrophils in periodontal inflammation. Front Oral Biol. 2012;15:56-83.  https://doi.org/10.1159/000329672
  11. Baeza M, Garrido M, Hernandez-Rios P, Dezerega A, Garcia-Sesnich J, Strauss F, Aitken JP, Lesaffre E, Vanbelle S, Gamonal J. Diagnostic accuracy for apical and chronic periodontitis biomarkers in gingival crevicular fluid: An exploratory study. J Clin Periodontol. 2016;43:34-45.  https://doi.org/10.1111/jcpe.12479
  12. Rai B, Kharb S, Jain R, Anand SC. Biomarkers of periodontitis in oral fluids. J Oral Sci. 2008;50(1):53-56.  https://doi.org/10.2334/josnusd.50.53
  13. Hernandez M, Gamonal J, Tervahartiala T, Mantyla P, Rivera O, Dezerega A, Dutzan N, Sorsa T. Associations between matrix metalloproteinase-8 and -14 and myeloperoxidase in gingival crevicular fluid from subjects with progressive chronic periodontitis: a longitudinal study. J Periodontol. 2010;81(11):1644-1652. https://doi.org/10.1902/jop.2010.100196
  14. Verstappen J, Hoff JW. Tissue inhibitors of metalloproteinases (TIMPs): Their biological functions and involvement in oral disease. J Dent Res. 2006;85(12):1074-1084. https://doi.org/10.1177/154405910608501202
  15. Chen D, Wang Q, Ma Z, Chen F, Chen Y, Xie G, Wang Q, Wu Z. MMP‐2, MMP‐9 and TIMP‐2 gene polymorphisms in Chinese patients with generalized aggressive periodontitis. J Clin Periodontol. 2007;34(5):384-389.  https://doi.org/10.1111/j.1600-051X.2007.01071.x
  16. Dong W, Xiang J, Li C, Cao Z, Huang Z. Increased expression of extracellular matrix metalloproteinase inducer is associated with matrix metalloproteinase-1 and -2 in gingival tissues from patients with periodontitis. J Periodontal Res. 2009;44(1):125-132.  https://doi.org/10.1111/j.1600-0765.2008.01105.x
  17. Boelen G-J, Boute L, d’Hoop J, EzEldeen M, Lambrichts I, Opdenakker G. Matrix metalloproteinases and inhibitors in dentistry. Clin Oral Investig. 2019;23(7):2823-2835. https://doi.org/10.1007/s00784-019-02915-y
  18. Tüter G, Serdar M, Kurtiş B, Walker S, Atak A, Toyman U, Pınar S, Aykan T. Effects of scaling and root planing and subantimicrobial dose doxycycline on gingival crevicular fluid levels of matrix metalloproteinase‐8, ‐13 and serum levels of HsCRP in patients with chronic periodontitis. J Periodontol. 2010;81(8):1132-1139. https://doi.org/10.1902/jop.2010.090694
  19. Guimaraes-Stabili MR, de Medeiros MC, Rossi D, Camilli AC, Zanelli CF, Valentini SR, Spolidorio LC, Kirkwood KL, Rossa C Jr. Silencing matrix metalloproteinase-13 (Mmp-13) reduces inflammatory bone resorption associated with LPS-induced periodontal disease in vivo. Clin Oral Investig. 2020. https://doi.org/10.1007/s00784-020-03644-3
  20. Yucel K, Afacan B, Emingil G, Tervahartiala T, Kose T, Sorsa T. Local and systemic levels of aMMP‐8 in gingivitis and stage 3 grade C periodontitis. J Periodontal Res. 2020;55(6):887-894.  https://doi.org/10.1111/jre.12781
  21. Kraft‐Neumärker M, Lorenz K, Koch R, Hoffmann T, Mäntylä P, Sorsa T, Netuschil L. Full‐mouth profile of active MMP‐8 in periodontitis patients. J Periodontal Res. 2012;47(1):121-128.  https://doi.org/10.1111/j.1600-0765.2011.01416.x
  22. Şurlin P, Oprea B, Solomon SM, Popa SG, Moţa M, Mateescu GO, Rauten AM, Popescu DM, Dragomir LP, Puiu I, Bogdan M, Popescu MR. Matrix metalloproteinase -7, -8, -9 and -13 in gingival tissue of patients with type 1 diabetes and periodontitis. Rom J Morphol Embryol. 2014;55(3 Suppl):1137-1141.
  23. Beklen A. Effects of IL-13 on TGF-β and MMP-1 in periodontitis. Biotech Histochem. 2017;92(5):374-380.  https://doi.org/10.1080/10520295.2017.1312526
  24. Wan C, Yuan G, Yang J, Sun Q, Zhang L, Zhang J, Zhang L, Chen Z. MMP9 deficiency increased the size of experimentally induced apical periodontitis. J Endod. 2014;40(5):658-664.  https://doi.org/10.1016/j.joen.2014.01.003
  25. Zhang H, Liu L, Jiang C, Pan K, Deng J, Wan C. MMP9 protects against LPS-induced inflammation in osteoblasts. Innate Immun. 2020;26(4):259-269.  https://doi.org/10.1177/1753425919887236
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.