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Kuznetsova O.A.

Russian Medical Academy of Continuous Professional Education

Zavalishina L.E.

Russian Medical Academy of Continuous Professional Education

Andreeva Yu.Yu.

Russian Medical Academy of Continuous Professional Education

Vinogradov M.I.

Regional Clinical Oncology Dispensary

Shomova M.V.

Regional Clinical Oncology Dispensary

Frank G.A.

Russian Medical Academy of Continuous Professional Education

Immunohistochemical study of MSI markers in breast cancer

Authors:

Kuznetsova O.A., Zavalishina L.E., Andreeva Yu.Yu., Vinogradov M.I., Shomova M.V., Frank G.A.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2021;83(1): 12‑17

DOI: 10.17116/patol20218301112

Views: 4362

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Table of contents

To cite this article:

Kuznetsova OA, Zavalishina LE, Andreeva YuYu, Vinogradov MI, Shomova MV, Frank GA. Immunohistochemical study of MSI markers in breast cancer. Russian Journal of Archive of Pathology. 2021;83(1):12‑17. (In Russ.)
https://doi.org/10.17116/patol20218301112

Подписка 2025 (Russian Journal of Archive of Pathology)
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OBJECTIVE

To investigate the expression of microsatellite instability (MSI) markers, which is detected by an immunohistochemical technique, and to compare the expression with the PD-L1 status in luminal B, HER2-negative and triple-negative breast cancer.

MATERIAL AND METHODS

The investigation included tumors from 40 patients with triple-negative and luminal B, HER2-negative subtypes. Immunohistochemical study was performed using Ventana antibodies: anti-MLH1 (clone M1), anti-MSH2 (clone G219-1129), anti-PMS2 (clone A16-4), and anti-MSH6 (clone SP93). MSI was assessed according to the standard criteria.

RESULTS

The PD-L1-positive status was present in 14 (35%) of the 40 patients. Moreover, MSI-H was detected in only 1 (2.50%) case. The two-year survival rate was 87.5%; it should be noted that the median survival rate was not reached either in the study sample or in the groups divided according to PD-L1 and MSI statuses. The overall survival rate for patients with MSI was 75% (3/4).

CONCLUSION

The first comparative study of the expression of PD-L1 and immunohistochemical MSI markers, which has been conducted on a small sample, fails to draw unambiguous conclusions, but shows the need to investigate this phenomenon on large samples and by using genetic methods.

Keywords:

breast cancer
PD-L1
SP142
MSI
microsatellite instability

Authors:

Kuznetsova O.A.

Russian Medical Academy of Continuous Professional Education

Zavalishina L.E.

Russian Medical Academy of Continuous Professional Education

Andreeva Yu.Yu.

Russian Medical Academy of Continuous Professional Education

Vinogradov M.I.

Regional Clinical Oncology Dispensary

Shomova M.V.

Regional Clinical Oncology Dispensary

Frank G.A.

Russian Medical Academy of Continuous Professional Education

Contribution of Authors:

The concept and design of the study — G.A. Frank

Collection and processing of material — M.I. Vinogradov, O.A. Kuznetsova

Statistical processing — O.A. Kuznetsova

Text writing — L.E. Zavalishina, Yu.Yu. Andreeva

Editing — M.V. Shomova, Yu.Yu. Andreeva

Received:

28.10.2020

Accepted:

16.11.2020

Conflict of Interest :

The authors declare no conflict of interest.

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