The morphological and molecular biological signs of impaired endometrial receptivity in infertility in women suffering from external genital endometriosis

Paramonova N.B.; Kogan E.A.; Kolotovkina A.V.; Burmenskaia O.V.

doi:https://doi.org/10.17116/patol201880311-18

Paramonova N.B.

FGBU "Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova" Minzdrava Rossii, Moskva;
GBOU "Pervyĭ Moskovskiĭ gosudarstvennyĭ meditsinskiĭ universitet im. I.M. Sechenova" Minzdrava Rossii, Rossiĭskaia Federatsiia

Kogan E.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Kolotovkina A.V.

Lapino Clinical Hospital

Burmenskaia O.V.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

The morphological and molecular biological signs of impaired endometrial receptivity in infertility in women suffering from external genital endometriosis

Authors:

Paramonova N.B., Kogan E.A., Kolotovkina A.V., Burmenskaia O.V.

More about the authors

Journal: Russian Journal of Archive of Pathology. 2018;80(3): 11‑18

DOI: 10.17116/patol201880311-18

Read: 2692 times

Download PDF (RU)

Contact the author

Table of contents

To cite this article:

Paramonova NB, Kogan EA, Kolotovkina AV, Burmenskaia OV. The morphological and molecular biological signs of impaired endometrial receptivity in infertility in women suffering from external genital endometriosis. Russian Journal of Archive of Pathology. 2018;80(3):11‑18. (In Russ.)
https://doi.org/10.17116/patol201880311-18

Подписка 2026 Архив патологии (Russian Journal of Archive of Pathology)

Использование инфографики авторами научных работ

Close metadata

Recommended articles:

Morphofunctional characteristics of the endometrium in patients of reproductive age with chronic salpingo-oophoritis. Russian Journal of Archive of Pathology. 2024;(6):49-57

The role of ferroptosis in reproduction. A modern view of the problem. Russian Journal of Human Reproduction. 2024;(5):35-45

Local cytokine profile of the endometrium in patients with repeated implantation failures and its pharmacological correction by the secretome of peripheral blood mononuclear cells. Russian Journal of Human Reproduction. 2024;(5):46-54

Clinical and anamnestic features of patients with recurrence of deep infiltrative endometriosis. Russian Journal of Human Reproduction. 2024;(5):89-100

The influence of endometriosis on the quality of life and reproductive function of modern woman. Russian Journal of Human Reproduction. 2024;(5):101-107

Efficiency of using dienogest at the stage of pregravid preparation on the course of pregnancy and the outcome of childbirth in patients with endometriosis. Russian Journal of Human Reproduction. 2024;(5):118-125

Comparison of the main methods of surgical treatment of patients with colorectal endometriosis. Russian Bulletin of Obstetrician-Gynecologist. 2024;(6):118-123

Oxidative stress, ferroptosis, somatic mutations, antioxidant therapy, and endometriosis: a new perspective on the issue. Russian Journal of Human Reproduction. 2024;(6):32-44

Modern view on the experimental creation of endometriosis in animal models (own data). Russian Journal of Human Reproduction. 2024;(6):55-60

Experimental modeling, prevention, and treatment of endometriosis-associated adhesion formation in NIH/3T3 fibroblast cell line using bovhyaluronidase azoximer (in vitro). Russian Journal of Human Reproduction. 2024;(6):61-72

Abstract:

Objective — to study endometrial receptivity in infertile women with external genital endometriosis (EGE). Subject and methods. Clinical, morphological, immunohistochemical, and molecular genetic examinations of endometrial aspiration pipelle biopsy specimens obtained on days 22—24 of the menstrual cycle from 94 infertile women with endometriosis: 50 women with Stage I-II EGE and 44 women with ovarian endometrioid cysts (OEC). A control group consisted of 54 women with tubal peritoneal factor of infertility (TPFI) and a successful attempt at IVF. Hematoxylin and eosin-stained sections were found to contain a number of endometrial surface epithelial cells containing mature pinopods. The expression levels of leukemia inhibitory factor (LIF), HOXA-10, glycodelin A, avβ3 integrin, estrogen receptors (ER) and progesterone receptors (PR), aromatase in the superficial epithelium, glandular epithelium and endometrial stroma were immunohistochemically revealed. Forty-four patients, including 17 with Stage I-II EGE and 27 with TPFI, showed mRNA expression levels of leukemia inhibitory factor receptor (LIFR), LIF, ER1, PgR, HOXA-10, and PTEN by real-time quantitative polymerase chain reaction (PCR) with a preliminary reverse transcription PCR assay. Results. It was established that in the infertile women with Stage I—II EGE and those with OEC, endometrial receptivity was impaired, which was manifested by a decline in the number of superficial epithelial cells containing mature pinopods, as well as a decrease in the endometrial level of the key receptivity markers: αvβ3 integrin, LIF, glycodelin A, and HOXA10 and increases in the synthesis of aromatase and in the imbalance of endometrial stromal expression of ER and PR detected by immunohistochemistry (IHC). Molecular genetic study showed lower mRNA expression levels of the HOXA-10, LIFR, and PgR genes, which confirms the data obtained by IHC.

Keywords:

endometriosis

infertility

endometrial receptivity

pinоpods

molecular markers of receptivity

Authors:

Paramonova N.B.

Kogan E.A.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Kolotovkina A.V.

Lapino Clinical Hospital

Burmenskaia O.V.

Nauchnyĭ tsentr akusherstva, ginekologii i perinatologii im. akad. V.I. Kulakova, Moskva

Close metadata

References:

Adamyan LV, Sonova MM, Tikhonova ES, Zimina EV, Antonova SO. The medical and social aspects of genital endometriosis. Problemy reproduktsii. 2011;17(6):78-81. (In Russ.)
Krasnopol’skaya KV, Nazarenko TA, Ershova IYu. Modern approaches to the evaluation of the endometrial receptivity (review). Problemy reproduktsii. 2016;22(5):61-69. (In Russ.) https://doi.org/10.17116/repro201622561-69
Shurshalina AV, Demura TA. Morphofunctional rearrangements of the endometrium during the implantation window. Akusherstvo i ginekologiya. 2011;(7-2):9-13. (In Russ.)
Kogan EA, Kalinina EA, Kolotovkina AV, Faizullina NM, Adamyan LV. Morphological and molecular substrate of impaired endometrial receptivity in infertile patients with external genital endometriosis. Akusherstvo i ginekologiya. 2014;8:47-52. (In Russ.)
Kogan EA, Kolotovkina AV, Faizulina NM, Kalinina EA. Endometrial morphological and molecular biological features during the implantation window in patients with infertility concurrent with Stages I—II external genital endometriosis. Akusherstvo i ginekologiya. 2013;9:35-40. (In Russ.)
Xu B, Sun X, Li L, Wu L, Zhang A, Feng Y. Pinopodes, leukemia inhibitory factor, integrin-β3, and mucin-1 expression in the peri-implantation endometrium of women with unexplained recurrent pregnancy loss. Fertil Steril. 2012;98(2):389-395. https://doi.org/10.1016/j.fertnstert.2012.04.032
Nikas G. Pinopodes as markers of endometrial receptivity in clinical practice. Hum Reprod. 1999;14(2):99-106.
Kogan EA, Nikolenko VN, Zanozin AS, Demura TA, Kolosovskii DYu. Syndrome of undifferentiated connective tissue dysplasia in combination with hereditary thrombophilia as the cause of primary female infertility. Meditsinskii vestnik Severnogo Kavkaza. 2016:11(2):323-326. (In Russ.) https://doi.org/10.14300/mnnc.2016.11067
Kogan EA, Demura TA, Vodyanoy VYa, Shurshalina AV. Molecular and morphological aspects of endometrial receptivity disorders at chronic endometritis. Arkhiv patologii. 2012;74(3):15-17. (In Russ.)
Wu M, Yin Y, Zhao M, Hu L,Chen Q. The low expression of leukemia inhibitory factor in endometrium: possible relevant to unexplained infertility with multiple implantation failures. Cytokine. 2013;6(2):334-339. https://doi.org/10.1016/j.cyto.2013.03.002
Novotny Z, Krizan J, Sima R, Sima P, Uher P, Zech N, Huttelova R, Baborova P, Ulchova-Gallova Z, Shubrt I, Ulmanova E, Houdek Z, Rokyta Z, Babushka V, Kralichkova M. Leukaemia inhibitory factor (LIF) gene mutations in women diagnosed with unexplained infertility and endometriosis have a negative impact on the IVF outcome. A pilot study. Folia Biol. (Praha). 2009;55:92-97.
Coughlan C, Sinagra M, Ledger W, Li TC, Laird S. Endometrial integrin expression in women with recurrent implantation failure after in vitro fertilization and its relationship to pregnancy outcome. Fertil Steril. 2013;100(3):825-830. https://doi.org/10.1016/j.fertnstert.2013.05.007
Casals G, Ordi J, Creus M, Fábregues F, Carmona F, Casamitjana R, Balasch J. Expression pattern of osteopontin and αvβ3 integrin during the implantation window in infertile patients with early stages of endometriosis. Hum Reprod. 2012;27(3):805-813. https://doi.org/10.1093/humrep/der432
Gude D. Glycodelin —Newer perceptions. J Hum Reprod Sci. 2011;4(3):156-157. https://doi.org/10.4103/0974-1208.92294
Lee CL, Lam KK, Koistinen H, Seppala M, Kurpisz M, Fernandez N, Pang RT, Yeung WS, Chiu PC. Glycodelin-A as a paracrine regulator in early pregnancy. J Reprod Immunol. 2011; 90(1):29-34. https://doi.org/10.1016/j.jri.2011.04.007
Szczepańska M, Wirstlein P, Luczak M, Jagodzinski PP, Skrzypczak J. Expression of HOXA-10 and HOXA-11 in the endometria of women with idiopathic infertility. Folia Histochem Cytobiol. 2011;49(1):111-118. https://doi.org/10.5603/FHC.2011.0016
Zanatta A, Rocha AM, Carvalho FM, Ricardo MA, Pereira RMA, Taylor HS, Motta EL, Baracat EC, Serafini PC. The role of the Hoxa-10/HOXA-10 gene in the etiology of endometriosis and its related infertility: a review. J Assist Reprod Genet. 2010;27(12):701-710. https://doi.org/10.1007/s10815-010-9471-y
Moberg C, Bourlev V, Ilyasova N, Olovsson M. Levels of oestrogen receptor, progesterone receptor and αB-crystallin in eutopic endometrium in relation to pregnancy in women with endometriosis. Hum Fertil (Camb). 2015;18(1):30-37. https://doi.org/10.3109/14647273.2014.922705
Bulun SE, Cheng YH, Pavone ME, Xue Q, Attar E, Trukhacheva E, Tokunaga H. Estrogen receptor-beta, estrogen receptor-alpha, and progesterone resistance in endometriosis. Semin Reprod Med. 2010;28(1):36-43. https://doi.org/10.1055/s-0029-1242991
Dyuzheva EV, Kogan EA, Kalinina EA, Kuz’michev LN. The principles of individual hormone preparation of the endometrium in patients with in-vitro fertilization failures. Akusherstvo i ginekologiya. 2011;7-2:39-45. (In Russ.)
Maia H Jr, Haddad C, Casoy J. Correlation between aromatase expression in the eutopic endometrium of symptomatic patients and the presence of endometriosis. Int J Women’s Health. 2012;4:61-65. https://doi.org/10.2147/IJWH.S29154
Adamyan LV, Zairat’yants OV, Maksimova YuV, Murdalova ZKh. New pathogenetic aspects of extended infiltrative endometriosis: theory and practice. Problemy reproduktsii. 2010;16(4):31-36. (In Russ.)