Immunohistochemical detection of biomolecular markers for metaplastic mucosal atrophy in gastric biopsy specimens

Kononov A.V.

GBOU "Omskaia gosudarstvennaia meditsinskaia akademiia" Ministerstva zdravookhraneniia Rossiĭskoĭ Federatsii

Mozgovoĭ S.I.

Kafedra patologicheskoĭ anatomii Omskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Shimanskaia A.G.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

Grishchenko R.K.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

Nazarov A.N.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

Immunohistochemical detection of biomolecular markers for metaplastic mucosal atrophy in gastric biopsy specimens

Authors:

Kononov A.V., Mozgovoĭ S.I., Shimanskaia A.G., Grishchenko R.K., Nazarov A.N.

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Journal: Russian Journal of Archive of Pathology. 2014;76(6): 44‑50

DOI: 10.17116/patol201476644-50

Downloaded: 115

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To cite this article:

Kononov AV, Mozgovoĭ SI, Shimanskaia AG, Grishchenko RK, Nazarov AN. Immunohistochemical detection of biomolecular markers for metaplastic mucosal atrophy in gastric biopsy specimens. Russian Journal of Archive of Pathology. 2014;76(6):44‑50. (In Russ.)
https://doi.org/10.17116/patol201476644-50

Подписка 2025 (Russian Journal of Archive of Pathology)

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Objective: to estimate the validity of the signs of metaplastic atrophic gastritis to elaborate a marker principle of its detection. Subjects and methods. Two hundred diagnostic cases morphologically diagnosed with chronic gastritis were selected for examination. The validity of the histological and immunohistochemical signs/markers reflecting a gland abnormality (hyperplasia of smooth muscle cells and argyrophilic and elastic fibers) and a cell phenotype change (intestinal and pyloric metaplasia): CDX-2, Shh, villin, CD10, MUC2, and MUC5AC was estimated in gastric biopsy specimens with atrophic gastritis forms verified in accordance with international classifications. The validity of the signs/markers was assessed, by calculating the sensitivity, specificity, prognostic value of positive and negative results, and positive and negative likelihood ratios. Results. There were 3 molecules: CDX-2 is a nuclear transcription factor associated with intestinal differentiation; CCD10 is a brush border membrane-bound mycin and MUC2 is an intestinal-type mycin, which showed a high validity like the markers of metaplastic atrophic gastritis. An algorithm that could probably evaluate atrophic gastritis was elaborated for the successive immunohistochemical identification of the above-mentioned marker. Conclusion. The proposed technical decision to verify atrophic gastritis by the biomarker method may be not an alternative, but complementary technique of identifying the form of atrophic gastritis.

Keywords:

biomolecular markers

gastric biopsy specimens

immunohistochemistry

metaplastic atrophy

chronic gastritis

Authors:

Kononov A.V.

GBOU "Omskaia gosudarstvennaia meditsinskaia akademiia" Ministerstva zdravookhraneniia Rossiĭskoĭ Federatsii

Mozgovoĭ S.I.

Kafedra patologicheskoĭ anatomii Omskoĭ gosudarstvennoĭ meditsinskoĭ akademii

Shimanskaia A.G.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

Grishchenko R.K.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

Nazarov A.N.

GBOU VPO "Omskaia gosudarstvennaia meditsinskaia akademiia" Minzdrava Rossii, Rossiĭskaia Federatsiia

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