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Shusharina N.N.

Immanuel Kant Baltic Federal University, Kaliningrad, Russia

Patrushev M.V.

Immanuel Kant Baltic Federal University, Kaliningrad, Russia

Silina E.V.

Kafedra patofiziologii FPPO vracheĭ Moskovskoĭ meditsinskoĭ akademii im. I.M. Sechenova

Stupin V.A.

GBOU VPO Rossiĭskiĭ natsional'nyĭ issledovatel'skiĭ meditsinskiĭ universitet im. N.I. Pirogova, kafedra gospital'noĭ khirurgii #1

Litvitsky P.F.

Chair of Hospital-Based Therapy №2, Chair of Pathophysiology of Medical Faculty, Sechenov First Moscow State Medical University of Ministry of Health of Russia, Moscow, Russia

Orlova A.S.

I.M. Sechenov First Moscow State Medical University, Moscow

Expression of genes for neurotransmitter transporters in astrocytes in different brain regions in experiment

Authors:

Shusharina N.N., Patrushev M.V., Silina E.V., Stupin V.A., Litvitsky P.F., Orlova A.S.

More about the authors

Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2018;118(6): 58‑64

DOI: 10.17116/jnevro20181186158

Read: 3338 times

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Table of contents

EXPRESSION OF GENES FOR NEUROTRANSMITTER TRANSPORTERS IN ASTROCYTES IN DIFFERENT BRAIN REGIONS IN EXPERIMENT
EXPRESSION OF GENES FOR NEUROTRANSMITTER TRANSPORTERS IN ASTROCYTES IN DIFFERENT BRAIN REGIONS IN EXPERIMENT

To cite this article:

Shusharina NN, Patrushev MV, Silina EV, Stupin VA, Litvitsky PF, Orlova AS. Expression of genes for neurotransmitter transporters in astrocytes in different brain regions in experiment. S.S. Korsakov Journal of Neurology and Psychiatry. 2018;118(6):58‑64. (In Russ.)
https://doi.org/10.17116/jnevro20181186158

Подписка 2026 Журнал неврологии и психиатрии им. С.С. Корсакова (S.S. Korsakov Journal of Neurology and Psychiatry)
 
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Abstract:

Objective. To investigate the expression of transporters of different neurotransmitters (glutamate, aspartate, lactate, choline) in the culture of astrocytes isolated from different regions of the brain (cortex, hippocampus and brainstem) in 3- and 11-day rats. Material and methods. An experimental study was performed on 24 3- (n=12) and 11-days (n=12) old rats (Rattus norvegicus). The results of high-performance sequencing were analyzed. Results. The expression of glutamate and aspartate transporters in the brainstem of 3-day rats was higher than in other regions, however, an opposite effect was observed in 11-day rats. The expression of lactate transporters with age became identical to those of the cortex. Conclusion. The data demonstrate the particular qualities of neuro-astrocytic connections and the important role of astrocytes in signal transmission. Results of the study performed by using genetic methods developed by the authors for the study of neurotransmitter transporters make it possible to recommend these methods to control the neurogenesis and neurohomeostasis, including in cerebrovascular and neurodegenerative diseases.

Keywords:

astrocytes
neuroglia
transporters
neurotransmitters
glutamate
aspartate
choline
lactate
gene expression
genetic methods of neurogenesis registration

Authors:

Shusharina N.N.

Immanuel Kant Baltic Federal University, Kaliningrad, Russia

Patrushev M.V.

Immanuel Kant Baltic Federal University, Kaliningrad, Russia

Silina E.V.

Kafedra patofiziologii FPPO vracheĭ Moskovskoĭ meditsinskoĭ akademii im. I.M. Sechenova

Stupin V.A.

GBOU VPO Rossiĭskiĭ natsional'nyĭ issledovatel'skiĭ meditsinskiĭ universitet im. N.I. Pirogova, kafedra gospital'noĭ khirurgii #1

Litvitsky P.F.

Chair of Hospital-Based Therapy №2, Chair of Pathophysiology of Medical Faculty, Sechenov First Moscow State Medical University of Ministry of Health of Russia, Moscow, Russia

Orlova A.S.

I.M. Sechenov First Moscow State Medical University, Moscow

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