Assessment of the levels of neuron-specific enolase and BDNF at the stages of rehabilitation in the acute and early recovery periods of ischemic stroke

Koroleva E.S.; Brazovskaya N.G.; Levchuk L.A.; Kazakov S.D.; Romadina N.Y.; Alifirova V.M.

doi:https://doi.org/10.17116/jnevro202012008230

Koroleva E.S.

Siberian State Medical University

SPIN RSCI: 6009-8113
Scopus AuthorID: 56299905400
ORCID: 0000-0003-1911-166X

Brazovskaya N.G.

Siberian State Medical University

Levchuk L.A.

Tomsk National Research Medical Center of the Russian Academy of Sciences

Kazakov S.D.

Siberian State Medical University

Romadina N.Y.

Tomsk Regional Clinical Hospital

Alifirova V.M.

Siberian State Medical University

SPIN RSCI: 3824-1016
Scopus AuthorID: 6507431329
ORCID: 0000-0002-4140-3223

Assessment of the levels of neuron-specific enolase and BDNF at the stages of rehabilitation in the acute and early recovery periods of ischemic stroke

Authors:

Koroleva E.S., Brazovskaya N.G., Levchuk L.A., Kazakov S.D., Romadina N.Y., Alifirova V.M.

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Journal: S.S. Korsakov Journal of Neurology and Psychiatry. 2020;120(8‑2): 30‑36

DOI: 10.17116/jnevro202012008230

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To cite this article:

Koroleva ES, Brazovskaya NG, Levchuk LA, Kazakov SD, Romadina NY, Alifirova VM. Assessment of the levels of neuron-specific enolase and BDNF at the stages of rehabilitation in the acute and early recovery periods of ischemic stroke. S.S. Korsakov Journal of Neurology and Psychiatry. 2020;120(8‑2):30‑36. (In Russ.)
https://doi.org/10.17116/jnevro202012008230

Подписка 2026 Журнал неврологии и психиатрии им. С.С. Корсакова. Спецвыпуски (S.S. Korsakov Journal of Neurology and Psychiatry (special issues))

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BACKGROUND

The issue of the diagnostic significance and clinical value of neuron-specific enolase (NSE) and brain-derived neurotropic factor (BDNF) in the acute period of stroke remains controversial. Therefore, it is advisable to study the correlation of biomarkers with the clinical characteristics of stroke in the time period of early recovery.

OBJECTIVE

To monitor NSE and BDNF levels in peripheral blood, to analyze the clinical and laboratory correlations in patients with ischemic stroke at the stages of medical rehabilitation in the early recovery period.

MATERIAL AND METHODS

Forty-nine patients with ischemic stroke in the middle cerebral artery were examined. The observation period is 90 days. Observation Points are Day 1; Day 14; Day 45; Day 90. The National Institute of Health Stroke Scale (NIHSS), the Fugle-Meyer Scale (FMA), the Modified Rankin Scale (mRS) were administered. NSE was determined in blood serum by enzyme-linked immunosorbent assay, BDNF was analyzed on a multiplex analyzer.

RESULTS AND CONCLUSION

NSE_Day₁ in patients was significantly higher than in the comparison group (p_{Day1—comparison group}<0.001) with a trend to a maximum decrease on the 90th day of stroke (p_Day1—90<0.001). BDNF_Day₁ turned out to be lower than in the comparison group (p_{Day1—comparison group}=0.006) and significantly increased by the 14th day of the stroke (p_Day1—14<0.001; p_{Day14—comparison group}=0.637). A negative correlation was found between a decrease in NSE_Day1₄and an increase in BDNF_Day1₄(r= –0.349; p=0.05). A positive correlation was found between an increase in BDNF_Day1₄ and a decrease in mRS scores _Day9₀ (r=0.499, p=0.035). Outcomes in patients in group 1 (after stages I and II of rehabilitation) on the assessment scales were significantly better than in patients discharged after stage I for outpatient monitoring — group 2 (p<0.05). In group 1, BDNF_Day9₀ did not differ from BDNF_Day1₄ (p_{Day14—90—Group1}=0.17), and in group 2 it was significantly lower by the end of the early recovery period (p_{Day14—90—Group2}=0.002).

Keywords:

ischemic stroke

neuron-specific enolase

brain-derived neurotropic factor

rehabilitation

early recovery period

Authors:

Koroleva E.S.

Siberian State Medical University

SPIN RSCI: 6009-8113
Scopus AuthorID: 56299905400
ORCID: 0000-0003-1911-166X

Brazovskaya N.G.

Siberian State Medical University

Levchuk L.A.

Tomsk National Research Medical Center of the Russian Academy of Sciences

Kazakov S.D.

Siberian State Medical University

Romadina N.Y.

Tomsk Regional Clinical Hospital

Alifirova V.M.

Siberian State Medical University

SPIN RSCI: 3824-1016
Scopus AuthorID: 6507431329
ORCID: 0000-0002-4140-3223

Received:

14.03.2020

Accepted:

15.03.2020

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References:

Whiteley W. Identifying blood biomarkers to improve the diagnosis of stroke. The Journal of the Royal College of Physicians of Edinburgh. 2011;41(2):152-154. https://doi.org/10.4997/jrcpe.2011.207
Jickling GC, Sharp FR. Blood biomarkers of ischemic stroke. Neurotherapeutics. 2011;8:349-360. https://doi.org/10.1007/s13311-011-0050-4
González-García S, González-Quevedo A, Peña-Sánchez M, Menéndez-Saínz C, Fernández-Carriera R, Arteche-Prior M, Pando-Cabrera A, Fernández-Concepción O. Serum neuron specific enolase and S100 calcium binding protein B biomarker levels do not improve diagnosis of acute stroke. J R Coll Physicians Edinb. 2012;42:199-204. https://doi.org/10.4997/JRCPE.2012.302
González-García S, González-Quevedo A, Fernández-Concepción O, Peña-Sánchez M, Menéndez-Saínz C, Hernández-Díaz Z, Arteche-Prior M, Pando-Cabrera A, Fernández-Novales C. Short-term prognostic value of serum neuron specific enolase and S100B in acute stroke patients. Clin Biochem. 2012;45(16-17):1302-1307. https://doi.org/10.1016/j.clinbiochem.2012.07.094
Gójska-Grymajło A, Zieliński M, Wardowska A, Gąsecki D, Pikuła M, Karaszewski B. CXCR7+ and CXCR4+ stem cells and neuron specific enolase in acute ischemic stroke patients. Neurochem Int. 2018;120:134-139. https://doi.org/10.1016/j.neuint.2018.08.009
Bharosay A, Bharosay VV, Saxena K, Varma M. Role of Brain Biomarker in Predicting Clinical Outcome in Hypertensive Cerebrovascular Ischemic Stroke. Indian J Clin Biochem. 2018;33(2):178-183. https://doi.org/10.1007/s12291-017-0664-3
Lasek-Bal A, Jedrzejowska-Szypulka H, Student S, Warsz-Wianecka A, Zareba K, Puz P, Bal W, Pawletko K, Lewin-Kowalik J. The importance of selected markers of inflammation and blood-brain barrier damage for short-term ischemic stroke prognosis. J Physiol Pharmacol. 2019;70(2): 45-49. https://doi.org/10.26402/jpp.2019.2.04
Cronberg T, Rundgren M, Westhall E, Englund E, Siemund R, Rosén I, Widner H, Friberg H. Neuron-specific enolase correlates with other prognostic markers after cardiac arrest. Neurology. 2011;77(7):623-630. https://doi.org/10.1212/WNL.0b013e31822a276d
Ahmad O, Wardlaw J, Whiteley WN. Correlation of levels of neuronal and glial markers with radiological measures of infarct volume in ischaemic stroke: A systematic review. Cerebrovasc Dis. 2012;33(1):47-54. https://doi.org/10.1159/000332810
Kaca-Oryńska M, Tomasiuk R, Friedman A. Neuron-specific enolase and S 100B protein as predictors of outcome in ischaemic stroke. Neurol Neurochir Pol. 2010;44(5):459-463. https://doi.org/10.1016/s0028-3843(14)60136-5
Lu K, Xu X, Cui S, Wang F, Zhang B, Zhao Y. Serum neuron specific enolase level as a predictor of prognosis in acute ischemic stroke patients after intravenous thrombolysis. J Neurol Sci. 2015;359(1-2):202-206. https://doi.org/10.1016/j.jns.2015.10.034
Zaheer S, Beg M, Rizvi I, Islam N, Ullah E, Akhtar N. Correlation between serum neuron specific enolase and functional neurological outcome in patients of acute ischemic stroke. Ann Indian Acad Neurol. 2013;16(4):504-508. https://doi.org/10.4103/0972-2327.120442
Balkaya M, Cho S. Genetics of stroke recovery: BDNF val66met polymorphism in stroke recovery and its interaction with aging. Neurobiol Dis. 2019;126:36-46. https://doi.org/10.1016/j.nbd.2018.08.009
Cohen-Cory S, Kidane AH, Shirkey NJ, Marshak S. Brain-derived neurotrophic factor and the development of structural neuronal connectivity. Dev Neurobiol. 2010;70(5):271-288. https://doi.org/10.1002/dneu.20774
Lasek-Bal A, Jędrzejowska-Szypułka H, Różycka J, Bal W, Holecki M, Duława J, Lewin-Kowalik J. Low concentration of BDNF in the acute phase of ischemic stroke as a factor in poor prognosis in terms of functional status of patients. Med Sci Monit. 2015;21:3900-3905. https://doi.org/10.12659/msm.895358
Stanne TM, Åberg ND, Nilsson S, Jood K, Blomstrand C, Andreasson U, Blennow K, Zetterberg H, Isgaard J, Svensson J, Jern C. Low circulating acute brain-derived neurotrophic factor levels are associated with poor long-term functional outcome after ischemic stroke. Stroke. 2016;47(7):1943-1945. https://doi.org/10.1161/STROKEAHA.115.012383
Clarkson AN, Overman JJ, Zhong S, Mueller R, Lynch G, Carmichael ST. AMPA receptor-induced local brain-derived neurotrophic factor signaling mediates motor recovery after stroke. J Neurosci. 2011;31(10):3766-3775. https://doi.org/10.1523/JNEUROSCI.5780-10.2011