The site of the Media Sphera Publishers contains materials intended solely for healthcare professionals.
By closing this message, you confirm that you are a certified medical professional or a student of a medical educational institution.

Login
EN
+7 (495) 482-4118
+7 (495) 482-4329
+8 800 250-18-12
  • (toll-free number for subscriptions)
    Mon-Fri from 10 a.m. to 6 p.m.

  • © 1998-2025
+7 (495) 482-43-29
EN
All journals
Journal
Year
Year
Search result: 0

Avetisov S.E.

FGBU "Nauchno-issledovatel'skiĭ institut glaznykh bolezneĭ" RAMN, Moskva

Abramova N.D.

Research Institute of Eye Diseases, Moscow, Russia

Gogoleva N.E.

Kazan Institute of Biochemistry and Biophysics — stand-alone branch of Kazan Scientific Center of RAS, Kazan, Russia;
Kazan Federal University (Volga Region), Institute of Fundamental Medicine and Biology, Kazan, Russia

Gusev O.A.

RIKEN-KFU Translational Genomics Unit, RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama City, Japan

Mitichkina T.S.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Novikov I.A.

Institute of Cardiology named after A.L. Myasnikov of National Medical Research Center for Cardiology, Moscow, Russia

Subbot A.M.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Shagimardanova E.I.

Kazan Federal University (Volga Region), Institute of Fundamental Medicine and Biology, Kazan, Russia

Rational strategy for studying microbiome of the ocular surface of people using hard contact lenses by method of 16S rRNA gene metabarcoding

Authors:

Avetisov S.E., Abramova N.D., Gogoleva N.E., Gusev O.A., Mitichkina T.S., Novikov I.A., Subbot A.M., Shagimardanova E.I.

More about the authors

Journal: Russian Annals of Ophthalmology. 2020;136(3): 3‑9

DOI: 10.17116/oftalma20201360313

Read: 2461 times

Download PDF (RU)
Download PDF
Contact the author
Table of contents

RATIONAL STRATEGY FOR STUDYING MICROBIOME OF THE OCULAR SURFACE OF PEOPLE USING HARD CONTACT LENSES BY METHOD OF 16S RRNA GENE METABARCODING
RATIONAL STRATEGY FOR STUDYING MICROBIOME OF THE OCULAR SURFACE OF PEOPLE USING HARD CONTACT LENSES BY METHOD OF 16S RRNA GENE METABARCODING

To cite this article:

Avetisov SE, Abramova ND, Gogoleva NE, et al. . Rational strategy for studying microbiome of the ocular surface of people using hard contact lenses by method of 16S rRNA gene metabarcoding. Russian Annals of Ophthalmology. 2020;136(3):3‑9. (In Russ., In Engl.)
https://doi.org/10.17116/oftalma20201360313

Подписка 2026 Вестник офтальмологии (Russian Annals of Ophthalmology)
МСФ на ЯМ
Использование инфографики авторами научных работ
Close metadata
Recommended articles:
Microbiota of the ocular surface in children with myopia. Russian Annals of Ophthalmology. 2025;(3):5-12
Gut microbiota: role in human health and pote­ntial for personalized medi­cine. Russian Journal of Evidence-Based Gastroenterology. 2024;(4):81-88
Aica­rdi—Goutieres syndrome type 6 asso­ciated with a compound hete­rozygous variant in ADAR: a first case report in the Russian popu­lation. S.S. Korsakov Journal of Neurology and Psychiatry. 2025;(1):131-138
Nutrition correction in the complex therapy and reha­bilitation of the posttraumatic stress diso­rder. Russian Journal of Preventive Medi­cine. 2025;(2):114-119
Factors supporting chro­nic inflammation in mental illness. S.S. Korsakov Journal of Neurology and Psychiatry. 2025;(8):7-15
Comparative characteristics of communities of microorganisms in the surface waters of bays in the coastal waters of the Sea of Japan expo­sed to anthropogenic impact. Mole­cular Gene­tics, Microbiology and Viro­logy. 2025;(3):36-46
«Brain—gut—microbiome» axis in patients with Parkinson’s disease. S.S. Korsakov Journal of Neurology and Psychiatry. 2025;(11-2):18-23
Abstract:

  1. Ozkan J, Nielsen S, Diez-Vives C, Coroneo M, Thomas T, Willcox M. Temporal stability and composition of the ocular surface microbiome. Scientific Reports. 2017;7(1):9880. https://doi.org/10.1038/s41598-017-10494-9
  2. Dong Q, Brulc JM, Iovieno A, Bates B, Garoutte A, Miller D, et al. Diversity of Bacteria at Healthy Human Conjunctiva. Invest Ophthalmol Visual Sci. 2011;52(8):5408-5413. https://doi.org/10.1167/iovs.10-6939
  3. Huang Y, Yang B, Li W. Defining the normal core microbiome of conjunctival microbial communities. Clin Microbiol Infect. 2016;22(7):643.e7-643.e12. https://doi.org/10.1016/j.cmi.2016.04.008
  4. Willcox M. Characterization of the normal microbiota of the ocular surface. Exper Eye Res. 2013;117:99-105. https://doi.org/10.1016/j.exer.2013.06.003
  5. Meisel JS, Hannigan GD, Tyldsley AS, SanMiguel AJ, Hodkinson BP, Zheng Q, et al. Skin microbiome surveys are strongly influenced by experimental design. J Invest Dermatol. 2016;136(5):947-956. https://doi.org/10.1016/j.jid.2016.01.016
  6. Herlemann DPR, Labrenz M, Jürgens K, Bertilsson S, Waniek JJ, Andersson AF. Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea. ISME J. 2011;5(10):1571-1579. https://doi.org/10.1038/ismej.2011.41
  7. Ozkan J, Coroneo M, Willcox M, Wemheuer B, Thomas T. Identification and visualization of a distinct microbiome in ocular surface conjunctival tissue. Invest Ophthalmol Vis Sci. 2018;59(10):4268-4276. https://doi.org/10.1167/iovs.18-24651
  8. Shin H, Price K, Albert L, Dodick J, Park L, Dominguez-Bello MG. Changes in the eye microbiota associated with contact lens wearing. MBio. 2016;7(2):e00198-16. https://doi.org/10.1128/mbio.00198-16
  9. Blinkova LP, Pakhomov YuD, Stoyanova LG. Properties of nonculturable and dormant forms of microorganisms. Immunopatologiya, allergologiya, infektologiya. 2010;(2):67-76. (In Russ.).
  10. Rinke C, Schwientek P, Sczyrba A, Ivanova NN, Anderson IJ, Cheng JF, et al. Insights into the phylogeny and coding potential of microbial dark matter. Nature. 2013;499(7459):431-437. https://doi.org/10.1038/nature12352
  11. Baranov AA, Mayansky AN, Chebotar IV, Mayansky NA. The new era in medical Microbiology. Vestnik Rossiyskoy akademii nauk. 2015;85(11):1011-1018. (In Russ.). https://doi.org/10.7868/S086958731511002X
  12. Oliver JD. The viable but nonculturable state in bacteria. J Microbiol. 2005; 43(1):93-100.
  13. Lloyd KG, Steen AD, Ladau J, Yin J, Crosby L. Phylogenetically novel uncultured microbial cells dominate Earth microbiomes. mSystems. 2018;3(5):1-12. https://doi.org/10.1128/mSystems.00055-18
  14. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016;13(7):581-583. https://doi.org/10.1038/nmeth.3869
  15. Brooks JP, Edwards DJ, Harwich MD Jr, Rivera MC, Fettweis JM, Serrano MG, et al. The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies. BMC Microbiol. 2015;15:66. https://doi.org/10.1186/s12866-015-0351-6
  16. Acinas SG, Sarma-Rupavtarm R, Klepac-Ceraj V, Polz MF. PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample. Appl Environ Microbiol. 2005; 71(12):8966-8969. https://doi.org/10.1128/AEM.71.12.8966-8969.2005
  17. Byrd AL, Belkaid Y, Segre JA. The human skin microbiome. Nat Rev Microbiol. 2018;16(3):143-155. https://doi.org/10.1038/nrmicro.2017.157
  18. Larkin DF, Leeming JP. Quantitative alterations of the commensal eye bacteria in contact lens wear. Eye (Lond). 1991;5(1):70-74. https://doi.org/10.1038/eye.1991.14
  19. Boost MV, Cho P. Microbial flora of tears of orthokeratology patients, and microbial contamination of contact lenses and contact lens accessories. Optom Vis Sci. 2005;82(6):451-458. https://doi.org/10.1097/01.opx.0000168587.72893.ec
  20. Szczotka-Flynn LB, Pearlman E, Ghannoum M. Microbial contamination of contact lenses, lens care solutions, and their accessories: a literature review. Eye Contact Lens. 2010;36(2):116-129. https://doi.org/10.1097/icl.0b013e3181d20cae.
  21. McAdam TG, Burnes BS. A pilot study of the effects of environmental and physiological stress on the conjunctival bacteria of college student contact wearers. BIOS. 2014;85(2):86-94. https://doi.org/10.1893/0005-3155-85.2.86
  22. Gao Z, Tseng CH, Pei Z, Blaser MJ. Molecular analysis of human forearm superficial skin bacterial biota. Proc Natl Acad Sci USA. 2007;104(8):2927-2932. https://doi.org/10.1073/pnas.0607077104

Keywords:

microbiome
ocular surface
contact lenses
sequencing

Authors:

Avetisov S.E.

FGBU "Nauchno-issledovatel'skiĭ institut glaznykh bolezneĭ" RAMN, Moskva

Abramova N.D.

Research Institute of Eye Diseases, Moscow, Russia

Gogoleva N.E.

Kazan Institute of Biochemistry and Biophysics — stand-alone branch of Kazan Scientific Center of RAS, Kazan, Russia;
Kazan Federal University (Volga Region), Institute of Fundamental Medicine and Biology, Kazan, Russia

Gusev O.A.

RIKEN-KFU Translational Genomics Unit, RIKEN Cluster for Science, Technology and Innovation Hub, Yokohama City, Japan

Mitichkina T.S.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Novikov I.A.

Institute of Cardiology named after A.L. Myasnikov of National Medical Research Center for Cardiology, Moscow, Russia

Subbot A.M.

FGBU "NII glaznykh bolezneĭ" RAMN, Moskva

Shagimardanova E.I.

Kazan Federal University (Volga Region), Institute of Fundamental Medicine and Biology, Kazan, Russia

Close metadata

References:

  1. Ozkan J, Nielsen S, Diez-Vives C, Coroneo M, Thomas T, Willcox M. Temporal stability and composition of the ocular surface microbiome. 2017;7(1):9880.
  2. Dong Q, Brulc JM, Iovieno A, Bates B, Garoutte A, Miller D, et al. Diversity of Bacteria at Healthy Human Conjunctiva. 2011;52(8):5408-5413.
  3. Huang Y, Yang B, Li W. Defining the normal core microbiome of conjunctival microbial communities. 2016;22(7):643.e7-643.e12. 
  4. Willcox M. Characterization of the normal microbiota of the ocular surface. 2013;117:99-105. 
  5. Meisel JS, Hannigan GD, Tyldsley AS, SanMiguel AJ, Hodkinson BP, Zheng Q, et al. Skin microbiome surveys are strongly influenced by experimental design. 2016;136(5):947-956. 
  6. Herlemann DPR, Labrenz M, Jürgens K, Bertilsson S, Waniek JJ, Andersson AF. Transitions in bacterial communities along the 2000 km salinity gradient of the Baltic Sea.  2011;5(10):1571-1579.
  7. Ozkan J, Coroneo M, Willcox M, Wemheuer B, Thomas T. Identification and visualization of a distinct microbiome in ocular surface conjunctival tissue. 2018;59(10):4268-4276.
  8. Shin H, Price K, Albert L, Dodick J, Park L, Dominguez-Bello MG. Changes in the eye microbiota associated with contact lens wearing. 2016;7(2):e00198-16. 
  9. Blinkova LP, Pakhomov YuD, Stoyanova LG. Properties of nonculturable and dormant forms of microorganisms. 2010;(2):67-76. (In Russ.).
  10. Rinke C, Schwientek P, Sczyrba A, Ivanova NN, Anderson IJ, Cheng JF, et al. Insights into the phylogeny and coding potential of microbial dark matter. 2013;499(7459):431-437. 
  11. Baranov AA, Mayansky AN, Chebotar IV, Mayansky NA. The new era in medical Microbiology. 2015;85(11):1011-1018. (In Russ.).
  12. Oliver JD. The viable but nonculturable state in bacteria. 2005; 43(1):93-100.
  13. Lloyd KG, Steen AD, Ladau J, Yin J, Crosby L. Phylogenetically novel uncultured microbial cells dominate Earth microbiomes. 2018;3(5):1-12. 
  14. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data. 2016;13(7):581-583. 
  15. Brooks JP, Edwards DJ, Harwich MD Jr, Rivera MC, Fettweis JM, Serrano MG, et al. The truth about metagenomics: quantifying and counteracting bias in 16S rRNA studies. 2015;15:66. 
  16. Acinas SG, Sarma-Rupavtarm R, Klepac-Ceraj V, Polz MF. PCR-induced sequence artifacts and bias: insights from comparison of two 16S rRNA clone libraries constructed from the same sample. 2005; 71(12):8966-8969.
  17. Byrd AL, Belkaid Y, Segre JA. The human skin microbiome. 2018;16(3):143-155. 
  18. Larkin DF, Leeming JP. Quantitative alterations of the commensal eye bacteria in contact lens wear. 1991;5(1):70-74. 
  19. Boost MV, Cho P. Microbial flora of tears of orthokeratology patients, and microbial contamination of contact lenses and contact lens accessories. 2005;82(6):451-458. 
  20. Szczotka-Flynn LB, Pearlman E, Ghannoum M. Microbial contamination of contact lenses, lens care solutions, and their accessories: a literature review. 2010;36(2):116-129. https://doi.org/10.1097/icl.0b013e3181d20cae.
  21. McAdam TG, Burnes BS. A pilot study of the effects of environmental and physiological stress on the conjunctival bacteria of college student contact wearers. 2014;85(2):86-94. 
  22. Gao Z, Tseng CH, Pei Z, Blaser MJ. Molecular analysis of human forearm superficial skin bacterial biota. 2007;104(8):2927-2932.
Contact the author
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.

Email Confirmation

An email was sent to test@gmail.com with a confirmation link. Follow the link from the letter to complete the registration on the site.

Email Confirmation

Contact us
If you have any questions, complaints or suggestions, please use this feedback form.
Email
Message
The publishing house "Media Sphere" does not provide treatment services, does not give recommendations on contacting medical institutions and specific specialists, on the prescription of medicines and dietary supplements.
Submit Application

You can become an author of one of our magazines, send a request and we will consider it in during the day.

Name
Phone
E-mail
Message
Login
Registration
E-mail
Password
Forgot Password?

Log in to the site using your account in one of the services

Password recovery
E-mail
Login
Register

We use cооkies to improve the performance of the site. By staying on our site, you agree to the terms of use of cооkies. To view our Privacy and Cookie Policy, please. click here.