Risk of type 2 diabetes mellitus in the Kyrgyz population in the presence of ADIPOQ (G276T), KCNJ11 (Glu23Lys), TCF7L2 (IVS3C&amp;gt;T) gene polymorphisms

Isakova Zh.T.; Talaibekova E.T.; Asambaeva D.A.; Kerimkulova A.S.; Lunegova O.S.; Aldasheva N.M.; Aldashev A.A.

doi:https://doi.org/10.17116/terarkh2017891040-47

Isakova Zh.T.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Talaibekova E.T.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Asambaeva D.A.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Kerimkulova A.S.

National Centre of Cardiology and Therapy, Bishkek, Kyrgyz Republic

Lunegova O.S.

National Centre of Cardiology and Therapy, Bishkek, Kyrgyz Republic

Aldasheva N.M.

Kyrgyz-Russian Slavic University, Bishkek, Kyrgyz Republic

Aldashev A.A.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Risk of type 2 diabetes mellitus in the Kyrgyz population in the presence of ADIPOQ (G276T), KCNJ11 (Glu23Lys), TCF7L2 (IVS3C>T) gene polymorphisms

Authors:

Isakova Zh.T., Talaibekova E.T., Asambaeva D.A., Kerimkulova A.S., Lunegova O.S., Aldasheva N.M., Aldashev A.A.

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Journal: Therapeutic Archive. 2017;89(10): 40‑47

DOI: 10.17116/terarkh2017891040-47

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Isakova ZhT, Talaibekova ET, Asambaeva DA, Kerimkulova AS, Lunegova OS, Aldasheva NM, Aldashev AA. Risk of type 2 diabetes mellitus in the Kyrgyz population in the presence of ADIPOQ (G276T), KCNJ11 (Glu23Lys), TCF7L2 (IVS3C>T) gene polymorphisms. Therapeutic Archive. 2017;89(10):40‑47. (In Russ.)
https://doi.org/10.17116/terarkh2017891040-47

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Abstract:

Aim. To analyze the association of genotype combinations of the polymorphic markers G276T in the ADIPOQ gene, Glu23Lys in the KCNJ11 gene, and IVS3C>T in the TCF7L2 gene with the development of type 2 diabetes mellitus (T2DM) in the Kyrgyz population. Subjects and methods. The investigation enrolled 23 Kyrgyz people, of whom there were 114 patients with T2DM and 109 without T2DM (a control group). T2DM was diagnosed in accordance with the WHO criteria (1999). The genotypes of ADIPOQ (G276T), KCNJ11 (Glu23Lys), and TCF7L2 (IVS3C>T) gene polymorphisms were identified using the restriction fragment length polymorphism analysis. Results. When typing at the polymorphic loci G276T in the ADIPOQ gene, Glu23Lys in the KCNJ11 gene, and IVS3C>T in the TCF7L2 gene, the development of T2DM in the Kyrgyz population was associated with the T allele (odds ratio (OR), 1.68; p=0.025), the heterozygous G276T genotype (OR 1,8; p=0.036) in the ADIPOQ gene; the 23Lys allele (OR, 1.62; p=0.019) in the KCNJ11 gene; a two-locus genotype combination in the genes ADIPOQ/KCNJ11: G276T/Glu23Lys (OR, 4.88; p=0.0013), G276G/Lys23Lys (OR, 4.65; p=0.019), G276T/Glu23Glu (OR, 3.10; p=0.022), a two-locus genotype combination in the genes ADIPOQ/TCF7L2: G276T/СС (OR, 1.97; p=0.04); two-locus genotype combinations in the genes KCNJ11/TCF7L2: Lys23Lys/CC (ОR, 2.65; p=0.042), Glu23Lys/CT (OR, 3.88; p=0.027); and a three-locus genotype combination in the genes ADIPOQ/KCNJ11/TCF7L2: G276T/Glu23Lys/CT (OR, 14.48; p=0.02). Conclusion. The development of T2DM in the Kyrgyz population is genetically determined by ADIPOQ (G276T) gene, KCNJ11 (Glu23Lys), and TCF7L (IVS3C>T) gene polymorphisms with the predisposing value of the T allele of the heterozygous G276T genotype in the ADIPOQ gene; the 23Lys allele in the KCNJ1 gene; as well as by genotype combinations in the genes ADIPOQ/KCNJ11 (G276T/Glu23Lys, G276G/Lys23Lys, G276T/Glu23Glu); ADIPOQ/TCF7L2 (G276T/SS); KCNJ11/TCF7L2 (Lys23Lys/CC, Glu23Lys/CT); ADIPOQ/KCNJ11/TCF7L2 (G276T/Glu23Lys /CT). The IVS3C>T locus in the TCF7L2 gene is not independently statistically significantly associated with the development of T2DM; however, its predisposing effect has been identified in its combination with the variant genotypes of the polymorphic loci G276T in the ADIPOQ gene and Glu23Lys in the KCNJ11 gene.

Keywords:

polymorphism

gene

ADIPOQ

KCNJ11

TCF7L2

type 2 diabetes mellitus

Kyrgyz population

Authors:

Isakova Zh.T.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Talaibekova E.T.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Asambaeva D.A.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

Kerimkulova A.S.

National Centre of Cardiology and Therapy, Bishkek, Kyrgyz Republic

Lunegova O.S.

National Centre of Cardiology and Therapy, Bishkek, Kyrgyz Republic

Aldasheva N.M.

Kyrgyz-Russian Slavic University, Bishkek, Kyrgyz Republic

Aldashev A.A.

Institute of Molecular Biology, Bishkek, Kyrgyz Republic

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