Spectroscopic studies of the molecular composition of the dentinal and gingival fluids and their diagnostic potential for preventive screening of dentin caries

Seredin P.V.; Goloshchapov D.L.; Ippolitov Yu.A.; Avraamova O.G.; Berkovich M.V.

doi:https://doi.org/10.17116/stomat20209905111

Seredin P.V.

Voronezh State University

Goloshchapov D.L.

Voronezh State University

Ippolitov Yu.A.

Voronezh State Medical University named after N.N. Burdenko

Avraamova O.G.

National Medical Research Center of Dentistry and Maxillofacial Surgery of the Ministry of Health of Russia

Berkovich M.V.

Voronezh State Medical University named after N.N. Burdenko

Spectroscopic studies of the molecular composition of the dentinal and gingival fluids and their diagnostic potential for preventive screening of dentin caries

Authors:

Seredin P.V., Goloshchapov D.L., Ippolitov Yu.A., Avraamova O.G., Berkovich M.V.

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Journal: Stomatology. 2020;99(5): 11‑18

DOI: 10.17116/stomat20209905111

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Seredin PV, Goloshchapov DL, Ippolitov YuA, Avraamova OG, Berkovich MV. Spectroscopic studies of the molecular composition of the dentinal and gingival fluids and their diagnostic potential for preventive screening of dentin caries. Stomatology. 2020;99(5):11‑18. (In Russ.)
https://doi.org/10.17116/stomat20209905111

Подписка 2026 Стоматология (Stomatology)

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PURPOSE OF THE STUDY

The aim of the work is a spectroscopic investigation and comparison of the molecular composition of dentine and gingival fluids as well as of their diagnostic potential for the preventive screening of dentin caries.

MATERIAL AND METHODS

Applying infrared spectroscopy including synchrotron radiation the samples of biological fluids taken from the oral cavity (dentine fluid, fluid from the gingival sulcus and blood) were studied for the patients with the progressive dentine caries. The part of this research was undertaken with the infrared microspectroscopy beamline at the Australian Synchrotron.

RESULTS

Dentine and gingival fluids were shown to have no less complicated composition than the blood serum. Having all this in mind, in spite that these two fluids are derivatives of the blood serum, and a majority of the molecular groups observed in all of the three fluids can be detected in their IR-spectra, it follows that there is a number of signature modes that are in fact present in the IR spectra of only dentine and gingival fluids. This fact indicates at the molecular exchange between them under certain conditions. It means the high diagnostic potential in the study of the pathologic processes occurring in the oral fluid of a human. We have observed and fixed an increase of thiocyanates and esters in the samples of both dentine and gingival fluids under the development of dentin caries.

CONCLUSION

Utilization of the gingival fluid for screening taking into account that its sampling is not such a complicated challenge as dentine fluid sampling will promote a transition to the personalized medicine, the development of high-technology healthcare and technology of the health preservation as a whole.

Keywords:

dentin fluid

gingival fluid

dentin caries

diagnostics

IR spectroscopy

Authors:

Seredin P.V.

Voronezh State University

Goloshchapov D.L.

Voronezh State University

Ippolitov Yu.A.

Voronezh State Medical University named after N.N. Burdenko

Avraamova O.G.

National Medical Research Center of Dentistry and Maxillofacial Surgery of the Ministry of Health of Russia

Berkovich M.V.

Voronezh State Medical University named after N.N. Burdenko

Received:

07.10.2019

Accepted:

17.01.2020

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References:

Rôças IN, Alves FRF, Rachid CTCC, Lima KC, Assunção IV, Gomes PN, et al. Microbiome of Deep Dentinal Caries Lesions in Teeth with Symptomatic Irreversible Pulpitis. PLoS One 2016;11:e0154653. https://doi.org/10.1371/journal.pone.0154653.
Mills A. Structural and Chemical Organization of Teeth. Elsevier; 2014.
Geraldeli S, Li Y, Hogan MMB, Tjaderhane LS, Pashley DH, Morgan TA, Zimmerman MB, Brogdenc KA. Inflammatory mediators in fluid extracted from the coronal occlusal dentine of trimmed teeth. Arch Oral Biol. 2012; 57:264-270. https://doi.org/10.1016/j.archoralbio.2011.08.012.
Tanner AC, Kressirer C, Faller L, Lake K, Dewhirst F, Kokarasb A, Paster B, Frias-Lopez J. Bacterial metatranscriptome of dentin caries. J Oral Microbiol. 2017;9:1325194. https://doi.org/10.1080/20002297.2017.1325194.
Muruppel AM. Laser-Assisted Diagnostics. In: Coluzzi D.J., Parker S.P.A., editors. Lasers in Dentistry — Current Concepts, Cham: Springer International Publishing; 2017. https://doi.org/10.1007/978-3-319-51944-9_6.
Goloshchapov DL, Kashkarov VM, Ippolitov YA, Prutskij T, Seredin PV. Early screening of dentin caries using the methods of Micro-Raman and laser-induced fluorescence spectroscopy. Results in Physics. 2018;10:346347. https://doi.org/10.1016/j.rinp.2018.06.040.
Seredin P, Goloshchapov D, Ippolitov Y, Pimm Vongsvivut. Pathology-specific molecular profiles of saliva in patients with multiple dental caries — potential application for predictive, preventive and personalised medical services. EPMA J. 2018;9:195-203. https://doi.org/10.1007/s13167-018-0135-9.
Gupta G. Gingival crevicular fluid as a periodontal diagnostic indicator- II: Inflammatory mediators, host-response modifiers and chair side diagnostic aids. J Med Life. 2013;6:7-13.
Bonnier F, Brachet G, Duong R, Sojinrin T, Respaud R, Aubrey N, et al. Screening the low molecular weight fraction of human serum using ATR-IR spectroscopy. J Biophotonics. 2016;9:10851097. https://doi.org/10.1002/jbio.201600015.
Bottoni U, Tiriolo R, Pullano SA, Dastoli S, Amoruso GF, Nisticò SP, Fiorillo AS. Infrared Saliva Analysis of Psoriatic and Diabetic Patients: Similarities in Protein Components. IEEE Transactions on Biomedical Engineering. 2016;63:379-384. https://doi.org/10.1109/TBME.2015.2458967.
Júnior C, Cesar P, Strixino JF, Raniero L, Júnior C, Cesar P, Strixino J F, Raniero L. Analysis of saliva by Fourier transform infrared spectroscopy for diagnosis of physiological stress in athletes. Res Biomed Engineer. 2015;31: 116-124. https://doi.org/10.1590/2446-4740.0664.
Ghosh A, Nishtala K. Biofluid lipidome: a source for potential diagnostic biomarkers. Clin Transl Med. 2017;6:22. https://doi.org/10.1186/s40169-017-0152-7.
Love RM, Jenkinson HF. Invasion of Dentinal Tubules by Oral Bacteria. Crit Rev Oral Biol Med. 2002;13:171-183. https://doi.org/10.1177/154411130201300207.
Vavilova TP. Biokhimiya tkanei i zhidkostei polosti rta. M.: GEOTAR-Media; 2019.
Seredin P, Goloshchapov D, Prutskij T, Ippolitov Y. Phase Transformations in a Human Tooth Tissue at the Initial Stage of Caries. PLoS ONE. 2015; 10:e0124008. https://doi.org/10.1371/journal.pone.0124008.
Parker F. Applications of Infrared Spectroscopy in Biochemistry, Biology, and Medicine. Springer Science & Business Media; 2012.
Makhnii T, Ilchenko O, Reynt A, Pilgun Y, Kutsyk A, Krasnenkov D, Ivasyuk M, Kukharskyy V. Age-Related Changes in FTIR and Raman Spectra of Human Blood. Ukrainian Journal of Physics. 2016;61:853-862. https://doi.org/10.15407/ujpe61.10.0853
Lopes J, Correia M, Martins I, Henriques AG, Delgadillo I, da Cruz e Silva O, Nunes A. FTIR and Raman Spectroscopy Applied to Dementia Diagnosis Through Analysis of Biological Fluids. J Alzheimer’s Dis. 2016;52: 801-112. https://doi.org/10.3233/JAD-151163.
Seredin P, Goloshchapov D, Kashkarov V, Ippolitov Y, Bambery K. The investigations of changes in mineral-organic and carbon-phosphate ratios in the mixed saliva by synchrotron infrared spectroscopy. Results in Physics. 2016;6:315-321. https://doi.org/10.1016/j.rinp.2016.06.005.
Xiang XM, Liu KZ, Man A, Ghiabi E, Cholakis A, Scott DA. Periodontitis-specific molecular signatures in gingival crevicular fluid. J Periodontal Res. 2010;45:345-352. https://doi.org/10.1111/j.1600-0765.2009.01243.x.
Matthäus C, Bird B, Miljković M, Chernenko T, Romeo M, Diem M. Infrared and Raman Microscopy in Cell Biology. Methods Cell Biol. 2008; 89:275-308. https://doi.org/10.1016/S0091-679X(08)00610-9.
Mikkonen JJW, Raittila J, Rieppo L, Lappalainen R, Kullaa AM, Myllymaa S. Fourier Transform Infrared Spectroscopy and Photoacoustic Spectroscopy for Saliva Analysis. Appl Spectrosc. 2016;70:1502-1510. https://doi.org/10.1177/0003702816654149.
Fujii S, Sato S, Fukuda K, Okinaga T, Ariyoshi W, Usui M. Diagnosis of Periodontal Disease from Saliva Samples Using Fourier Transform Infrared Microscopy Coupled with Partial Least Squares Discriminant Analysis. Anal Sci. 2016;32:225-231. https://doi.org/10.2116/analsci.32.225.
Almhöjd US, Norén JG, Arvidsson A, Nilsson Å, Lingström P. Analysis of Carious Dentine using FTIR and ToF-SIMS. Oral Health Dent Manag. 2014;13:735-744. https://doi.org/10.4172/2247-2452.1000666.
Balducci L, Ramachandran A, Hao J, Narayanan K, Evans C, George A. Biological Markers for Evaluation of Root Resorption. Arch Oral Biol. 2007; 52:203-208. https://doi.org/10.1016/j.archoralbio.2006.08.018.